A5100382105- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- Amino Acid Enzymes and Metabolism
- Process Optimization and Integration
- Tea Polyphenols and Effects
- Polyamine Metabolism and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Biochemical and Molecular Research
- Lipid metabolism and biosynthesis
- Histone Deacetylase Inhibitors Research
- Diet, Metabolism, and Disease
- Photosynthetic Processes and Mechanisms
- Chemical and Physical Properties in Aqueous Solutions
- Transgenic Plants and Applications
- Fermentation and Sensory Analysis
- Fungal and yeast genetics research
- Catalysis for Biomass Conversion
- Biotin and Related Studies
- Crystallization and Solubility Studies
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
Tianjin University of Science and Technology
2015-2024
Tianjin Medical University
2020-2023
Key Laboratory of Guangdong Province
2019
Ministry of Education of the People's Republic of China
2018
Tianjin Economic-Technological Development Area
2017
State Key Laboratory of Medicinal Chemical Biology
2016
Nankai University
2016
East China University of Science and Technology
2012
Corynebacterium glutamicum is an important industrial microorganism, but the availability of tools for its genetic modification has lagged compared to other model microorganisms such as Escherichia coli. Despite great progress in CRISPR-based technologies, most feasible genome editing method C. suicide plasmid-mediated, efficiency which low due high false-positive rates sacB counter selection, and requirement tedious two-round selection verification rare double-cross-over events. In this...
Corynebacterium glutamicum has industrial track records for producing a variety of valuable products such as amino acids. Although CRISPR-based genome editing technologies have undergone immense developments in recent years, the suicide-plasmid-based approaches are still predominant C. manipulation. It is crucial to develop simple and efficient CRISPR method glutamicum.In this study, we developed RecombinAtion Prior Induced Double-strand-break (RAPID) technology glutamicum, Cpf1 cleavage was...
In the present study, a novel breeding strategy of atmospheric and room temperature plasma (ARTP) mutagenesis was used to improve uridine production engineered Bacillus subtilis TD12np. A high-throughput screening method established using both resistant plates 96-well microplates select ideal mutants with diverse phenotypes. Mutant F126 accumulated 5.7 30.3 g/L after 30 h in shake-flask 48 fed-batch fermentation, respectively, which represented 4.4- 8.7-fold increase over parent strain....
l-Valine, an essential amino acid, serves as a valuable compound in various industries. However, engineering strains with both high yield and purity are yet to be delivered for microbial l-valine production. We engineered
Carbon competition between cell growth and product synthesis is the bottleneck in efficient N-acetyl glucosamine (GlcNAc) production microbial factories. In this study, a xylose-induced T7 RNA polymerase–PT7 promoter system was introduced Escherichia coli W3110 to control GlcNAc synthesis. Meanwhile, an arabinose-induced CRISPR interference (CRISPRi) applied adjust by attenuating transcription of key growth-related genes. By designing proper sgRNAs, followed elaborate adjustment addition...
Currently, microbial production is becoming a competitive method for N-acetyl-glucosamine production. As the biosynthesis of originating from fructose-6-P directly competes with central carbon metabolism precursor supply, consumption glucose cell growth and cellular severely limits yield N-acetyl-glucosamine. In this study, appropriate catabolic division labor in utilization mixed sources was achieved by deleting pfkA gene enhancing glycerol introducing glpK mutant. Glycerol thus mainly...
Alpha-ketobutyrate has been widely used in medicine and food additive industry. Because chemical enzymatic methods are associated with many deficiencies, the recent focus shifted to fermentation for production of α-ketobutyrate. In this study, a genetically engineered strain THRDΔrhtAΔilvIH/pWSK29-ilvA was constructed, starting from an L-threonine-producing strain, by overexpressing threonine dehydratase (TD), reducing α-ketobutyrate catabolism L-threonine export. The shake flask cultivation...
d-Amino acids (d-AAs) have wide applications in industries such as pharmaceutical, food, and cosmetics due to their unique properties. Currently, the production of d-AAs has relied on chemical synthesis or enzyme catalysts, it is challenging produce via direct fermentation from glucose. We observed that Corynebacterium glutamicum exhibits a remarkable tolerance high concentrations d-Ala, crucial characteristic for establishing successful process. By optimizing meso-diaminopilmelate...
L-theanine is the most abundant free amino acid in tea that offers various favorable physiological and pharmacological effects. Bacterial enzyme of γ-glutamylmethylamide synthetase (GMAS) can catalyze synthesis theanine from glutamate, ethylamine ATP, but manufacturing cost uncompetitive due to expensive substrates complex processes. In this study, we described pathway engineering wild-type Escherichia coli for one-step fermentative production sugars ethylamine. First, synthetic was...
Shikimate is a valuable chiral precursor for synthesizing oseltamivir (Tamiflu®) and other chemicals. High production of shikimate via microbial fermentation has attracted increasing attention to overcome the unstable expensive supply extracted from plant resources. The current cost engineered strains still unsatisfactory, thus more metabolic strategies need be investigated further increase efficiency. In this study, we first constructed E. coli producer through application...
The supplementation of betaine, an osmoprotective compatible solute, in the cultivation media has been widely used to protect bacterial cells. To explore effects betaine addition on industrial fermentation, Escherichia coli THRD, l-threonine producer, was examine production with and underlying mechanism through which functions investigated. Betaine medium E. THRD significantly improved fermentation parameters. transcription zwf corresponding enzyme activity glucose-6-phosphate dehydrogenase...