A5032728765- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Adipose Tissue and Metabolism
- Nutritional Studies and Diet
- Autophagy in Disease and Therapy
- Fatty Acid Research and Health
- Tracheal and airway disorders
- Endoplasmic Reticulum Stress and Disease
- Genomics and Rare Diseases
- Protein Hydrolysis and Bioactive Peptides
- Pulmonary Hypertension Research and Treatments
- Redox biology and oxidative stress
- RNA Interference and Gene Delivery
- Inflammasome and immune disorders
- MicroRNA in disease regulation
- Signaling Pathways in Disease
- Epigenetics and DNA Methylation
- Cell death mechanisms and regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant responses to water stress
- Animal Genetics and Reproduction
- Caveolin-1 and cellular processes
- Sperm and Testicular Function
University of Belgrade
2012-2022
Ollscoil na Gaillimhe – University of Galway
2016-2020
Institute of Molecular Genetics and Genetic Engineering
2006-2019
Institute of Molecular Genetics
2010-2015
Alpha-1-antitrypsin (A1AT) is the most abundant liver-derived, highly polymorphic, glycoprotein in plasma. Hereditary deficiency of alpha-1-antitrypsin plasma (A1ATD) a consequence accumulation polymers A1AT mutants endoplasmic reticulum hepatocytes and other A1AT-producing cells. One clinical manifestations A1ATD liver disease childhood cirrhosis and/or hepatocellular carcinoma (HCC) adulthood. Epidemiology pathophysiology failure early caused by are well known, but association with not...
Abstract The inflammasome is a multiprotein complex assembled in response to Pathogen Associated Molecular Patterns (PAMPs) and Danger (DAMPs). Inflammasome activation occurs through two-step mechanism, with the first signal facilitating priming of components while second triggers assembly. Once assembled, recruits activates pro-caspase-1, which turn processes pro-interleukin (IL)-18 pro-IL-1β into their bio-active forms. Owing its key role regulation innate immune responses, has emerged as...
Sperm chromatin compaction in the sperm head is achieved when histones are replaced by protamines during spermatogenesis. Haploinsufficiency of protamine 1 (PRM1) or PRM2 gene causes infertility mice. However, published data remain inconclusive about a role PRM1/2 variants male and their association with semen parameters. By full sequence analysis, we assessed frequency variations PRM1 three groups Caucasian patients idiopathic teratozoospermia normal (n = 88) reduced concentration 83) men...
The aim of the study was to investigate association between GSTM1 and GSTT1 gene deletion MTHFR C677T mutation male infertility. has encompassed 52 infertile 56 fertile males. Genotype distributions deletions did not differ significantly among analyzed groups, however, a difference in distribution certain genotype combinations observed. obtained results indicate that carriers double GSTM1/GSTT1 677CC are at higher risk infertility than any other combination genotypes (OR 3.5, 95%CI 0.68-18.30). .
Drug resistance presents a major obstacle to the successful treatment of glioblastoma. Autophagy plays key role in drug resistance, particularly relation targeted therapy, which has prompted use autophagy inhibitors increase effectiveness therapeutics. The ability two Src tyrosine kinase inhibitors, Si306 and its prodrug pro-Si306, induce was evaluated human glioblastoma cell line U87 multidrug-resistant counterpart U87-TxR. markers were assessed by flow cytometry, microscopy, Western blot,...
We have developed a relational database of human SERPINA1 gene mutations, leading to 1antitrypsin (AAT) deficiency, called A 1 ATVar, which can be accessed over the World Wide Web at www.goldenhelix.org/A1ATVar.Extensive information has been extracted from literature and converted into searchable database, including genotype information, clinical phenotype, allelic frequencies for commonest AAT variant alleles, methods detection, references.Mutation summaries are automatically displayed...
Abstract Background Although still considered a safer alternative to classical cigarettes, growing body of work points harmful effects electronic cigarettes (e-cigarettes) affecting range cellular processes. The biological effect e-cigarettes needs be investigated in more detail considering their widespread use. Methods In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration e-cigarette liquids, and without nicotine. Mutagenicity was evaluated by HPRT assay,...
This study has investigated a potential role of common Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene variants in the etiology noncystic fibrosis bronchiectasis Serbian children. The encompassed 48 patients (19 male and 29 female, aged between 5 18 years, median age 10.6±3.3), diagnosed with idiopathic based on high-resolution computed tomography thorax pathologic examination lobectomy materials. CFTR analysis was performed genomic DNA extracted from peripheral blood samples...
Tspan14 is a transmembrane protein of the tetraspanin (Tspan) family. Different members Tspan family can promote or suppress tumor progression. The exact role in cells unknown. Earlier, mutational inactivation TSPAN14 gene has been proposed to coincide with low survival rate NSCLC patients. This study aimed investigate correlation lack function clinicopathological features patients, and elucidate might have expression was lower than non-tumor patients' samples. decreased correlated patients...
The alpha-1-antitrypsin (A1AT) gene is highly polymorphic, with more than 100 genetic variants identified of which some can affect A1AT protein concentration and/or function and lead to pulmonary liver disease. This study reports on the characterization a p.G320R variant found in two patients, one emphysema other lung cancer. results from single base-pair substitution exon 4 gene, has been characterized as P by isoelectric focusing. Functional evaluation was through comparing specific...
Introduction: Alpha-1-antitrypsin deficiency (AATD), genetic risk factor for premature chronic obstructive pulmonary disease (COPD), often remains undetected. The aim of our study was to analyse the effectiveness an integrative laboratory algorithm AATD detection in patients diagnosed with COPD by age 45 years, comparison screening approach based on AAT concentration measurement alone.
Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein encoded by SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated anti-apoptotic property against non-malignant cells, we aimed to investigate whether affects apoptosis colon cell line (HCT116). The presence of HCT116 culture antagonized cytotoxicity blockers MEK1/2,...
This paper reports a novel Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene variant, 1811+1G→T, detected in 5-year-old girl diagnosed with idiopathic disseminated bronchiectasis and negative sweat chloride test (17 mmol/L). The performed CFTR mutation analysis included detection of the F508del mutation, Tn polymorphism screening exons 3, 10 11. has shown altered band pattern exon DNA sequencing 11 revealed presence sequence variation 1811+1G→T heterozygous state. was not found...