A5080304368- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Cell Image Analysis Techniques
- Biosensors and Analytical Detection
- Psychotherapy Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Peptidase Inhibition and Analysis
- Psychoanalysis and Psychopathology Research
- Computational Drug Discovery Methods
- Microfluidic and Capillary Electrophoresis Applications
- COVID-19 Clinical Research Studies
- Internet Traffic Analysis and Secure E-voting
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Network Security and Intrusion Detection
- Influenza Virus Research Studies
- Transactional Analysis in Psychotherapy
- Historical and Scientific Studies
- Clinical Laboratory Practices and Quality Control
- Neural dynamics and brain function
- Transgenic Plants and Applications
- Cystic Fibrosis Research Advances
- Supramolecular Self-Assembly in Materials
- Innovation Policy and R&D
- Microbial Natural Products and Biosynthesis
Scripps (United States)
2019-2023
The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology
2023
Scripps Research Institute
2007-2022
Scripps Institution of Oceanography
2019
Institute of Molecular Medicine
2017
Université du Québec à Trois-Rivières
2005-2012
Ecolab (United States)
2011
Florida Atlantic University
2005
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in late 2019 has triggered an ongoing global pandemic whereby infection may result a lethal pneumonia-like disease designated as (COVID-19). To date, millions confirmed cases and hundreds thousands deaths have been reported worldwide, there are currently no medical countermeasures available to prevent or treat the disease. purported development vaccine could require at least 1–4 years, while typical timeline from...
The severe acute respiratory syndrome coronavirus 2 responsible for COVID-19 remains a persistent threat to mankind, especially the immunocompromised and elderly which vaccine may have limited effectiveness. Entry of SARS-CoV-2 requires high affinity interaction viral spike protein with cellular receptor angiotensin-converting enzyme 2. Novel mutations on correlate transmissibility new variants SARS-CoV-2, highlighting need small molecule inhibitors virus entry into target cells. We report...
Recent technological advances have enabled 3D tissue culture models for fast and affordable HTS. We are no longer bound to 2D anti-cancer agent discovery, it is clear that tumor provide more predictive data translation of preclinical studies. In a previous study, we validated microplate spheroid-based technology its compatibility with HTS automation. Small-scale screens using approved drugs demonstrated drug responses tend differ between cancer cell proliferation models. Here, applied this...
Affordable and physiologically relevant three-dimensional (3D) cell-based assays used in high-throughput screening (HTS) are on the rise early drug discovery. These technologies have been aided by recent adaptation of novel microplate treatments spheroid culturing techniques. One such technology involves use nanoparticle (NanoShuttle-PL) labeled cells custom magnetic drives to assist cell aggregation ensure rapid 3D structure formation after dispensed into microtiter plates. Transitioning...
The SARS coronavirus 2 (SARS-CoV-2) pandemic remains a major problem in many parts of the world and infection rates remain at extremely high levels. This prevalence drives continued emergence new variants, possibly ones that are more vaccine-resistant can drive infections even highly vaccinated populations. rate variant evolution makes clear need for therapeutics be clinically applied to minimize or eliminate effects COVID-19. With hurdle 10 years, on average, first class small molecule...
Kinases are important drug discovery targets for a wide variety of therapeutic indications; consequently, the measurement kinase activity remains common high-throughput screening (HTS) application.Recently, enzyme-coupled luciferase-kinase (LK) format assays have been introduced.This measures luminescence resulting from metabolism adenosine triphosphate (ATP) via luciferin/luciferase-coupled reaction.In research presented here, 1536-well time-resolved fluorescence resonance energy transfer...
The Scripps Research Molecular Screening Center (SRMSC) was founded in 2004 and comprises more than $22 million of specialized automation. As part the Translational Institute (TRI), it early drug discovery labs medicinal chemistry. Together with at La Jolla, California, campus, this represents one most competitive academic industrial screening centers worldwide. SRMSC uses automated platforms, a cell other cherry-picking platform. Matched technologies are available throughout to allow...
Critical to maintaining quality control in high-throughput screening is the need for constant monitoring of liquid-dispensing fidelity. Traditional methods involve operator intervention with gravimetric analysis monitor gross accuracy full plate dispenses, visual verification contents, or dedicated weigh stations on platforms that introduce potential bottlenecks and increase plate-processing cycle time. We present a unique solution using open-source hardware, software, 3D printing automate...
Non-small cell lung cancer (NSCLC) is the most common type of and accounts for ∼84% all cases. NSCLC remains one leading causes cancer-associated death, with a 5-year survival rate less than 25%. This begins healthy cells that change start growing out control, to formation lesions or tumors. Understanding dynamics how tumor microenvironment promotes initiation progression leads metastasis crucial help identify new molecular therapies. 3D primary models have received renewed recognition due...
There is a pressing need to improve approaches for drug discovery related neuropsychiatric disorders (NSDs). Therapeutic in would benefit from screening assays that can measure changes complex phenotypes linked disease mechanisms. However, traditional track neuronal phenotypes, such as connectivity, exhibit poor scalability and are not compatible with high-throughput (HTS) procedures. Therefore, we created phenotypic assay platform focused on improving the affordability of neuron-based...
Autism Spectrum Disorder (ASD) is a heterogeneous neurodevelopmental disorder. There are no drugs to treat the core symptoms. De novo mutations often play an important role in ASD and multiple high-risk loci have been identified last decade. These range from copy number variants small insertion/deletion single nucleotide variants. Large-scale exome sequencing has over 100 risk genes that associated with ASD. Both etiological heterogeneity unavailability of human neurons remain major hurdles...
This report presents the high-resolution image acquisition and processing instrument for compound management applications (HIAPI-CM). The HIAPI-CM combines imaging spectroscopy machine-vision analysis to perform rapid assessment of high-throughput screening (HTS) library quality. It has been customized detect classify typical artifacts found in HTS microtiter plates (MTPs). These include (1) insufficient volume liquid sample, (2) precipitation, (3) colored compounds that interfere with assay...
NR2F6 is considered an orphan nuclear receptor since its endogenous ligand has yet to be identified. Recently, emerged as a novel cancer therapeutic target. been demonstrated upregulated or overexpressed in several cancers. Importantly, Nr2f6−/− mice spontaneously reject tumors and develop host-protective immunological memory, consequence of acting immune checkpoint effector T cells. Collectively, these data suggest that modulation activity may have important clinical applications the fight...
Cystic fibrosis (CF), an inherited genetic disease, is caused by mutation of the Fibrosis Transmembrane Conductance Regulator (CFTR) gene, which encodes ion channel involved in hydration maintenance anion homeostasis. Ninety percent CF patients possess one or more copies F508del CFTR mutation. This disrupts trafficking protein to plasma membrane and diminishes function mature CFTR. Identifying small molecule modulators mutant activity biosynthesis may yield new tools for discovering novel...
Microplates are an essential tool used in laboratories for storing research materials and performing assays. Many types of laboratory automation exist that greatly reduce the effort needed to utilize microplates; however, there cases where use such is not feasible or practical. In these instances, researchers must work environment liquid handling operations performed manually with handheld pipetting devices. This type tedious error-prone as it relies on track a significant amount metadata,...
Open-source projects continue to grow in popularity alongside open-source educational resources, software, and hardware tools. The impact of this increased availability technologies is that end users are empowered have greater control over the tools they work with. This trend extends life science laboratory space, where new routinely being published allow build modify scientific equipment specifically tailored their needs, often at a reduced cost from equivalent commercial...