A5055244525- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Childhood Cancer Survivors' Quality of Life
- Hematopoietic Stem Cell Transplantation
- Digestive system and related health
- Vitamin D Research Studies
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Clinical Nutrition and Gastroenterology
- Research on Leishmaniasis Studies
- Immunodeficiency and Autoimmune Disorders
- Biomedical Ethics and Regulation
- CAR-T cell therapy research
- Helicobacter pylori-related gastroenterology studies
- Parvovirus B19 Infection Studies
- Cancer Cells and Metastasis
- Liver physiology and pathology
- Cytomegalovirus and herpesvirus research
- Cytokine Signaling Pathways and Interactions
- Viral-associated cancers and disorders
Weill Cornell Medicine
2023-2024
Cornell University
2022-2024
Center for Global Health
2024
Memorial Sloan Kettering Cancer Center
2013-2019
Kettering University
2014-2015
T cell–derived interferon-γ can directly target intestinal stem cells to induce their apoptosis in a JAK/STAT-dependent manner (see the related Focus by Kretzschmar and Clevers ).
Abstract Background Reasons for the high prevalence of Kaposi sarcoma–associated herpesvirus (KSHV) in sub-Saharan Africa, and risk factors leading to viral reactivation shedding, remain largely undefined. Preliminary studies have suggested that schistosome infection, which has been associated with impaired control, is KSHV. In this study we sought determine relationship between active Schistosoma mansoni or haematobium infection KSHV shedding. Methods We quantified DNA saliva cervical swabs...
IL-22+ Group 3 innate lymphoid cells (ILC3s) are critical for maintaining intestinal epithelial integrity. However, GVHD leads to elimination of ILC3s, ILC deficiency is associated with increased clinical GVHD, and IL-22 experimental GVHD-related mortality, pathology, loss stem (ISCs). We thus evaluated the mechanisms ILC3s in regeneration protection from GVHD. Utilizing an ex vivo organoid culture system, we found that co-culture murine small intestine (SI) crypts significantly growth...
Abstract Innate lymphoid cells (ILC) are a newly described population of immune implicated in the maintenance and function tissues as diverse liver, gut, lung lymph nodes. We have recently key role for ILC, their production IL-22, endogenous thymic regeneration: crucial process that allows renewal competence following depletion. Unlike other cells, ILC were extremely radio-resistant with little depletion after even lethal doses total body irradiation. Consistent these findings, considerable...
Little is known about regulation of the intestinal stem cell (ISC) compartment in gut GVHD. We have found that Interleukin-22 (IL-22) important for ISC recovery after BMT. However, mechanism and specific epithelial targets IL-22 are poorly understood. Using clinically modeled LP into C57BL/6 (B6) minor antigen-mismatched BMT (H-2b H-2b), we daily treatment with recombinant murine (4ug IP starting D7 post-BMT) led to reduced GVHD pathology small intestine (SI) large (LI) three weeks...
Factors regulating damage and regeneration of the intestinal epithelium after allogeneic BMT are poorly understood. We have previously shown that IL-22 produced by recipient-derived innate lymphoid cells (ILCs) provides a critical signal for epithelial recovery following experimental BMT. However, levels reduced in GVHD due to elimination radioresistant host ILCs. therefore sought determine if administration post-BMT could negate effect ILC reduce pathology. utilized clinically modeled LP...
Innate lymphoid cells (ILCs) are associated with protective mucosal response after experimental allogeneic BMT. However, the specific in vivo roles of ILCs and their capacity for reconstitution post-transplant remain unclear. We therefore sought to characterize significance tissue distribution allo-BMT.To study role allo-BMT, we established a clinically relevant acute GVHD model based on H-Y antigen mismatch. Lethally irradiated Rag2−/− male mice (Thy1.2, B6) were transplanted T cell...
Abstract Little is known about regulation of the intestinal stem cell (ISC) compartment after injury. We hypothesized that IL-22 could promote regeneration by either acting on ISCs or Paneth cells (PCs) making up ISC niche. Utilizing an in vitro model, we found organoids derived from murine small intestine (SI) and large (LI) crypts demonstrated substantially increased size culture with IL-22. Innate lymphoid co-culture also organoid IL-22-dependent fashion. expanded Lgr5+ pool within SI...
Mechanisms leading to the loss of crypt base intestinal stem cells (ISCs) and their Paneth cell (PC) niche in GI GVHD are poorly understood. To assess relationship between ISC injury, we evaluated compartment epithelial regeneration after MHC- miHA-mismatched experimental allo-BMT. Lgr5+ ISCs were rapidly depleted pretransplant conditioning, but quickly recovered recipients T-cell-depleted (TCD) BMT by day 10 post-BMT. However, failed recover mice. preceded damage PC niche, suggesting as...