Acidification of the extracellular milieu malignant tumors is reported to increase invasive behavior cancer cells. In normal tissues, production acid catalyzed by carbonic anhydrases (CAs), some which are known be overexpressed in certain cancers. To investigate functional role CA activity such cells, we analyzed effect acetazolamide, a potent inhibitor, on capacity four renal carcinoma cell lines (Caki-1, Caki-2, ACHN, and A-498). We found that 10 μM acetazolamide inhibited relative...

Human salivary carbonic anhydrase (HCA VI) was purified by inhibitor affinity chromatography and its location in the human parotid submandibular glands identified, using a polyclonal antiserum raised against enzyme rabbits conjunction with peroxidase-antiperoxidase complex method. The antibodies did not crossreact HCA II or I. However, they slightly recognized IgA; therefore absorbed IgA before immunohistochemical use. VI-specific staining detected cytoplasm particularly secretory granules...

The distribution of carbonic anhydrase isoenzymes I, II, and VI was studied in the human alimentary tract using specific antibodies to conjunction with immunoperoxidase technique elucidate physiological role possible functional interplay anhydrases (CAs) canal functions. From studied, CA II found be most widely distributed various epithelia throughout canal. In addition acinar cells parotid submandibular glands duodenal Brunner's glands, it present mucosal epithelium oesophagus, stomach,...

Carbonic anhydrase isozyme XII (CA XII) is a novel membrane-associated protein with potential role in von Hippel-Lindau carcinogenesis. Although Northern blotting has revealed positive signal for CA normal human kidney, this the first study to demonstrate its cellular and subcellular localization along nephron collecting duct. Immunohistochemistry polyclonal antibody (PAb) raised against truncated distinct staining basolateral plasma membrane of epithelial cells thick ascending limb Henle...

Autoradiographic studies showed a specific uptake of 125I-LH in the corpus luteum pregnancy and theca cell layer follicle rat murine ovary. Both autoradiographic quantitative indicated that luteal receptor(s) for LH can be blocked by unlabeled LH, HCG anti-LH serum, but not with FSH. The LH-receptor was found particle preparation, presumably plasma membrane fraction. Intact cells also binding LH. After sonication fraction complex could separated from free hormone gel filtration...

Although long suspected from histochemical evidence for carbonic anhydrase (CA) activity on neurons and observations that CA inhibitors enhance the extracellular alkaline shifts associated with synaptic transmission, an in brain had not been identified. A candidate this was suggested by recent discovery of membrane (CA XIV) whose mRNA is expressed mouse human several other tissues. For immunolocalization XIV brain, we developed two antibodies, one against a secretory form enzymatically...

In addition to essential nutrients, human milk contains several classes of bioactive factors such as enzymes, hormones, and growth factors, many which are implicated in infantile development. Secretory carbonic anhydrase isoenzyme VI (CA VI) has been identified earlier an component mammalian saliva, we demonstrate here by using biochemical immunohistochemical techniques that it is also elementary milk. The 42-kDa glycopolypeptide purified from CA inhibitor affinity chromatography shared 100%...

Carbonic anhydrase IV (CA IV) is a glycosylphosphatidylinositol-linked isozyme previously identified on the surface of renal tubular epithelium and certain populations vascular endothelium. This report identifies regional, cellular, subcellular localization CA in rat gut. Northern blot RT-PCR analyses demonstrated little expression stomach or proximal small intestine, but abundant distal large intestine. In contrast, II mRNA was stomach, decreased low I detected only The regional...

The molecular mechanism governing the regulated secretion of most exocrine tissues remains elusive, although VAMP8/endobrevin has recently been shown to be major vesicular SNARE (v-SNARE) zymogen granules pancreatic acinar cells. In this article, we have characterized role VAMP8 in entire system. Immunohistochemical studies showed that is expressed all examined such as salivary glands, lacrimal (tear) sweat sebaceous mammary and prostate. Severe anomalies were observed glands VAMP8-null...

LH(hCG) receptors were identified with [125I]hCG in 38 benign and 26 malignant ovarian tumors of the human ovary. Eighteen per cent all 27% receptor-positive. Four 12 serous 3 17 mucinous displayed definitive binding [125I]hCG. Two Brenner failed to bind Six 21 epithelial Only one out 4 granulosa cell bound [125I]hCG, while other sex cord stromal as well dysgerminoma receptor content receptor-positive was low compared normal tissue. The presence certain may be a sign gonadotropic control...

Carbonic anhydrases maintain pH homeostasis in various tissues of the human body by catalyzing reversible reaction CO<sub>2</sub> + H<sub>2</sub>O <=> HCO<sub>3</sub><sup>–</sup> H<sup>+</sup>. anhydrase isoenzyme VI (CA VI) is secreted into saliva serous acinar cells parotid and submandibular glands. Although it represents about 3% total protein stimulated saliva, its exact physiological significance has not been established....

Salivary carbonic anhydrase (CA VI) appears to protect teeth from caries via mechanisms other than direct regulation of salivary pH and buffering capacity. To elucidate whether CA VI acts in the local microenvironment tooth surface, we studied location activity enzyme human enamel pellicle. The study was performed using a specific rabbit antiserum conjunction with immunostaining immunoblot techniques. demonstrated histochemical staining method. extracted having vivo formed pellicle showed...

The luteinizing hormone receptor (LHR) is a G protein-coupled involved in regulation of ovarian and testicular functions. Here we show that the present also specific areas peripheral central nervous system may thus have broader functional role than has been anticipated. Full-length LHR mRNA two protein species Mr 90,000 73,000, representing mature precursor forms, respectively, were expressed adult developing rat tissue, starting at fetal day 14.5. was capable ligand binding because it...

Electron microscopic radioautography has been utilized for subcellular localization of 125IhCG in ovarian cells rats which pseudopregnancy had honnonally induced. Light showed the highest concentration silver grains over luteal cells, with a more limited number thecal cells. Granulosa were generally free grains. radioautographs revealed that majority (72%) and was located at plasma membranes these No qualitative difference terms observed between samples obtained 5 or 60 min after injection...

17 beta-Hydroxysteroid dehydrogenase (17HSD) catalyzes the reversible conversion of estrone into estradiol. The complementary DNA (cDNA) coding for rat 17HSD type 1 was cloned from a commercial ovarian cDNA library, using human as probe. nucleotide sequence extends 1160 basepairs (bp), including 1035 bp open reading frame, stop codon, and 125 3'-untranslated sequence. encodes protein 344 amino acids, with calculated molecular mass 36,967 daltons. overall acid identity similarity between...

Salivary carbonic anhydrase VI (CA VI) appears to contribute taste function by protecting receptor cells (TRCs) from apoptosis. The serous von Ebner's glands locating in the posterior tongue deliver their saliva into bottom of trenches surrounding TRC-rich circumvallate and foliate papillae. Because these directly immediate vicinity TRCs, we investigated whether CA is secreted glands, using immunochemical techniques. immunohistochemical results showed that present acinar cells, ductal...