In addition to essential nutrients, human milk contains several classes of bioactive factors such as enzymes, hormones, and growth factors, many which are implicated in infantile development. Secretory carbonic anhydrase isoenzyme VI (CA VI) has been identified earlier an component mammalian saliva, we demonstrate here by using biochemical immunohistochemical techniques that it is also elementary milk. The 42-kDa glycopolypeptide purified from CA inhibitor affinity chromatography shared 100%...

The molecular mechanism governing the regulated secretion of most exocrine tissues remains elusive, although VAMP8/endobrevin has recently been shown to be major vesicular SNARE (v-SNARE) zymogen granules pancreatic acinar cells. In this article, we have characterized role VAMP8 in entire system. Immunohistochemical studies showed that is expressed all examined such as salivary glands, lacrimal (tear) sweat sebaceous mammary and prostate. Severe anomalies were observed glands VAMP8-null...

Salivary carbonic anhydrase (CA VI) appears to protect teeth from caries via mechanisms other than direct regulation of salivary pH and buffering capacity. To elucidate whether CA VI acts in the local microenvironment tooth surface, we studied location activity enzyme human enamel pellicle. The study was performed using a specific rabbit antiserum conjunction with immunostaining immunoblot techniques. demonstrated histochemical staining method. extracted having vivo formed pellicle showed...

Klinisk relevans De oftest forekommende intraorale skader, der kan komme fra udførelse af mundhygiejne i hjemmet, er tandslid, erosioner, gingivale retraktioner og dental fluorose. Ætiologien bag ved de enkelte tilstande sygdomme kendt. tidlige stadier erosioner fluorose svære at erkende klinisk. Ved første erkendelse tandslid bør patienten blive informeret om anvende tandpasta med lav slibeeffekt samt en hensigtsmæssig tandbørsteteknik. reduktion indtag syrlige fødeemner/drikke nødvendigt...

ABSTRACT Objectives We investigated the impact of time pressure on dentists' arousal levels and immediate quality composite resin fillings using two filling techniques in a simulated clinical setting. Material Methods Forty‐two dentists dental students were randomized to fill an identical Class II cavity either with bulk‐fill base technique or conventional incremental technique. The task was performed first under timing then pressure. Arousal 100 mm visual analog scale (VAS) wireless...

Artikkelissa kuvataan suuhygieniakäytänteiden mahdollisia haittavaikutuksia ja niiden välttämistä. Haitat luokitellaan neljään eri kategoriaan: abraasio, eroosio, ikenien vetäytyminen fluoroosi. Abraasiovaurioiden synnyssä keskeistä on hammastahnan hankaavuus. Tyypillinen abraasiovaurio hampaankaula-alueen kulumisvaurio, jota kutsutaan usein harjausvaurioksi. Ruoasta mahalaukusta peräisin olevat hapot demineralisoivat hampaita, mikä edistää abraasiota, koska hapon pehmentämä hammas alttiimpi...

Abstract Objective The study aims to evaluate the effect of contouring instruments on porosity and immediate quality direct dental restorations. Materials methods Fifteen human molars with 30 Class II 10 V cavities were restored by five voluntary dentists using three (conventional steel, silicone-tipped diamond-like carbon coated-instruments) filling materials (Admira Fusion, Filtek Supreme XTE Fuji LC). restorations evaluated for quality, number pores stereomicroscope micro-computed...

Salivary carbonic anhydrase VI (CA VI) appears to contribute taste function by protecting receptor cells (TRCs) from apoptosis. The serous von Ebner's glands locating in the posterior tongue deliver their saliva into bottom of trenches surrounding TRC-rich circumvallate and foliate papillae. Because these directly immediate vicinity TRCs, we investigated whether CA is secreted glands, using immunochemical techniques. immunohistochemical results showed that present acinar cells, ductal...

The aims of this randomised controlled laboratory trial were to determine the procedure time and immediate quality (surface porosity marginal gaps) fillings placed using bulk-fill base technique conventional incremental in simulated clinical settings.Forty-two dentists dental students randomly allocated use either or fill an identical class II disto-occlusal cavity a maxillary left first molar typodont tooth. We recorded participants used evaluated surface gaps on approximal surfaces...

This cross-sectional study aimed to examine oral health and health-related habits among prisoners at the Pelso Prison in Finland.Participants this comprises 100 inmates. A calibrated dentist recorded decayed, filled missed teeth as well periodontal status (bleeding-on-probing, pocket probing Community Periodontal Index) participants (n=100). Fifty inmates were also interviewed for marital status, education habits. The Ethical Committee of Northern Ostrobothnia Hospital District Criminal...

Abstract Dental caries, gingivitis, and excess weight are highly prevalent, interconnected chronic conditions. The association of oral health with the development adiposity among children is sparsely addressed. We examined to central obesity in early adolescence during a 2-year follow-up period. This prospective study was conducted 2702 aged 9–12 years at baseline from Finnish Health Teens study. Their followed up for 2 years. Body mass index age- sex-specific cut-offs waist–height ratio...

Carbonic anhydrase isoenzyme VI (CA VI), which is transported in high concentrations saliva and milk into the alimentary tract, an important element of mucosal protection upper tract. Like tract mucosa, respiratory mucosa also exposed to heavy microbial, physical, chemical stress. The protective renewal-promoting factors present surface mucus are mainly produced by seromucous tracheobronchial glands. Here we studied secretion CA these glands adult developing rats using immunohistochemical...

The aim of this retrospective data collection study was to determine the effect size dry mouth on survival restorations and teeth.The were collected from electronic patient files City Oulu Public Dental Services (Finland). Study population consisted 71 patients 142 control patients. 3208 analyzed using Kaplan-Meier curves multivariate Cox regression analysis with shared frailty for Separate analyses performed hazard ratio in 2.08 (95% CI: 1.65-2.63) compared For patients, fixed prosthetic...

Abstract We examined cross-sectional and longitudinal associations of dietary factors with caries experience in a population sample 487 children aged 6–9 years at baseline examinations the Physical Activity Nutrition Children (PANIC) Study. Altogether, 406 these attended 2-year follow-up examinations. Food consumption eating frequency were assessed using 4-day food records, diet quality Baltic Sea Diet Score (BSDS) behaviour Children’s Eating Behavior Questionnaire. Caries was clinically....

Erosive tooth wear is defined as irreversible loss of dental tissues due to intrinsic or extrinsic acids, exacerbated by mechanical forces. Recent studies have suggested a higher prevalence erosive in males, well genetic contribution susceptibility wear. Our aim was examine performing genome-wide association study (GWAS) sample the Northern Finland Birth Cohort 1966 (<i>n</i> = 1,962). assessed clinically using basic examination. A GWAS performed for whole separately males and...

Abstract Objectives This study aimed to compare the knowledge attained by third‐year dental students in physical ergonomics altering live lectures and videos teaching. The second aim was investigate implementation of theoretical on into practice. Material methods Forty‐five divided two groups attended a lecture (45 min) or viewed min). After first teaching session, changed parts. All answered questionnaire with 13 true false‐questions at baseline immediately after both sessions. Friedman's...

Excess sugar consumption—common in youth—is associated with poor health. Evidence on the relationship between consumption and oral microbiome, however, remains scarce inconclusive. We explored whether diversity, composition, functional capacities of saliva microbiota differ based select sugary foods drinks (“sweet treats”). Using 16S rRNA gene sequencing, we characterized from 11 to 13-year-old children who participated Finnish Health Teens (Fin-HIT) cohort study. The sample comprised lowest...

Abstract Background The concept of minimal intervention dentistry (MID) includes both delayed restorative treatment and conservative caries removal, is now recognised as an evidence-based approach for dental management. In order to determine if professionals in Russia are incorporating this into their clinical practice, we investigated the decisions Russian dentists students, factors associated with these decisions. Methods We included 171 general practitioners therapists (collectively...

<title>Abstract</title> Objective This study aimed to determine the survival of class II composite restorations with and without base bulk-fill in general dental practice. Materials Methods We collected data from electronic patient files Public Dental Services City Oulu, Finland. The consisted both composite. compared these using Kaplan-Meier curves, log-rank test, rates, Wilcoxon signed ranks test. Results observed 297 96 patients. five-year rates for were comparable premolars (77.5% 77.4%,...