A5011221591- Pain Mechanisms and Treatments
- Angiogenesis and VEGF in Cancer
- Axon Guidance and Neuronal Signaling
- Neuropeptides and Animal Physiology
- Plant tissue culture and regeneration
- Chemical Synthesis and Analysis
- Atmospheric chemistry and aerosols
- Chemical Reaction Mechanisms
- Analytical Chemistry and Chromatography
- Cholinesterase and Neurodegenerative Diseases
- Pharmacological Effects of Natural Compounds
- Synthesis and Reactions of Organic Compounds
- Estrogen and related hormone effects
- Advanced Chemical Sensor Technologies
- Organic and Inorganic Chemical Reactions
- Cancer, Hypoxia, and Metabolism
- Free Radicals and Antioxidants
- Medicinal Plants and Neuroprotection
- Dye analysis and toxicity
- Nicotinic Acetylcholine Receptors Study
- Air Quality and Health Impacts
- Advanced Proteomics Techniques and Applications
- melanin and skin pigmentation
- Ginkgo biloba and Cashew Applications
- Seed and Plant Biochemistry
University of Warsaw
2014-2023
Institute of Organic Chemistry
2011-2015
Polish Academy of Sciences
2011-2015
The present study concerns the identification of nine thus-far unknown derivatives carminic acid extracted from pre-Columbian Peruvian textiles dyed with American cochineal-these are not found in commercially available preparations dye. These compounds probably represent a unique fingerprint this region, as they have never been reported to occur other fabrics historical value. They were separated by reversed-phase high-performance liquid chromatography (phenyl column) and detected using...
Abstract. Aliphatic alcohols (AAs), including terpenoic (TAs), are ubiquitous in the atmosphere due to their widespread emissions from natural and anthropogenic sources. Hydroxyl radical (OH) is most important atmospheric oxidant both aqueous gas phases. Consequently, oxidation of TAs by OH inside clouds fogs a potential source secondary organic aerosols (aqSOAs). However, kinetic data, necessary for estimating timescales such reactions, remain limited. Here, bimolecular rate coefficients...
To cope with environmental harmful conditions, plant cells developed adaptive strategy that involves production of a wide variety complex secondary metabolites. The spectrum and quantity biosynthesized compounds in specific species is determined by its genotype, tissue, developmental physiological stage factors. This phenomenon was used to exploit the potential anatomical hairy root cultures Rindera graeca produce bioactive compounds. Cultivated vitro roots were subjected abiotic stresses...
AMA Czapski G, Szypuła W, Kudlik M, et al. Original articleAssessment of antioxidative activity alkaloids from Huperzia selago and Diphasiastrum complanatum using in vitro systems. Folia Neuropathologica. 2014;52(4):394-406. doi:10.5114/fn.2014.47840. APA Czapski, G., Szypuła, W., Kudlik, M., Wileńska, B., Kania, & Danikiewicz, W. (2014). Neuropathologica, 52(4), 394-406. https://doi.org/10.5114/fn.2014.47840 Chicago Grzegorz A., Wojciech Magdalena Beata Witold Agata Adamczyk. 2014....
Based on the mechanism of neuropathic pain induction, a new type bifunctional hybrid peptidomimetics was obtained for potential use in this pain. Hybrids consist two types pharmacophores that are connected by different linkers. The first pharmacophore is an opioid agonist, and second antagonist pronociceptive system, i.e., melanocortin-4 receptor. results tests acute models compounds have shown linker used to connect had effect antinociceptive activity. Peptidomimetics containing longer...
Abstract The purpose of our work was to determine the role nonopioid peptides derived from opioid prohormones in sensory hypersensitivity characteristics neuropathic pain and propose a pharmacological approach restore balance these endogenous systems. Nonopioid may have pronociceptive effect therefore contribute less effective analgesia pain. In study, we used unilateral chronic constriction injury (CCI) sciatic nerve as model rats. We demonstrated effects proopiomelanocortin-...
The in vitro cultures of Rindera graeca, a rare endemic plant, were developed as sustainable source phenolic acids. Various shoot and root established scaled up sprinkle bioreactor. A multiplication rate 7.2 shoots per explant was achieved. HPLC–PDA–ESI–HRMS analysis revealed the presence rosmarinic acid (RA) lithospermic B (LAB) main secondary metabolites both cultures. maximum RA (30.0 ± 3.2 mg/g DW) LAB (49.3 ±15.5 yields determined root-regenerated shoots. strongest free radical...
Neuropilin‐1 (NRP‐1) has been found to be overexpressed in several kinds of malignant tumors, and it is postulated that pathological angiogenesis, its interaction with the vascular endothelial growth factor 165 (VEGF ) leads progression tumor vascularization growth. Herein, we present synthesis vitro evaluation tetrapeptides general sequence K xx R, where represents two canonical amino acid residues or a single possessing hydrocarbon backbone. All synthesized compounds were inhibitors VEGF...
Inhibiting the interaction of neuropilin-1 (NRP-1) with vascular endothelial growth factor (VEGF) has become an interesting mechanism for potential anticancer therapies. In our previous works, we have obtained several submicromolar inhibitors this interaction, including branched pentapeptides general structure Lys(Har)-Xxx-Xxx-Arg. With intent to improve proteolytic stability inhibitors, turned attention 1,4-disubstituted 1,2,3-triazoles as peptide bond isosteres. present contribution,...
This is the first report of an efficient and effective procedure to optimize biosynthesis huperzine A (HupA) B (HupB) in vitro from Huperzia selago gametophytes. Axenic tissue cultures were established using spores collected sporophytes growing wild. The prothalia obtained after 7–18 months. Approximately 90 up 100% gametophytes viable grew rapidly each transfer on a fresh medium every 3 best biomass growth index for prothallus calculated (FW) dry weight (DW) basis, at 24 weeks culture, was...
Hairy root cultures are considered as a valuable source of bioactive phytoconstituents with expanding applicability for their production. In the present study, hairy Polyscias filicifolia (Araliaceae), traditional Southeast Asian medicinal plant, were established. The transformation Agrobacterium rhizogenes ATCC 15834 allowed to obtain 15 lines. K-1 line, demonstrating highest growth capabilities, was subjected further investigations. To enhance biosynthetic potential roots, methyl jasmonate...
Gas‐phase reactions of isomeric nitrophenide ions and p ‐halonitrophenide with acrylonitrile, methyl acrylate, ethyl acrylate have been studied using mass spectrometry computational methods. Depending on the structure α,β‐unsaturated compound, formation adducts to carbonyl group (for acrylate) β‐adducts (adducts compounds in β position) was observed. Further transformations these lead products elimination an alcohol molecule anionic intramolecular substitution a halogen atom, respectively....
Mass spectrometry imaging (MSI) can provide accurate data containing the spatial distribution of endogenous peptides in tissue sections without previous treatment. One key issues analyzing small samples is establishing a proper technique for mounting and manipulating collected order to avoid contamination sample with optimal cutting temperature (OCT) resin.We present method embedding rat pituitary frozen egg yolk block, which enables its further experiments on matrix-assisted laser...
Gas phase reactions of the substituted phenide ions with methyl formate have been studied. It was found that results these depend mainly on basicity ion, which is related to presence electron‐accepting or electron‐donating substituents in benzene ring. shown electron‐withdrawing groups react gas a two‐step reaction. The first step proceeds according typical addition–elimination mechanism formation anion respective benzaldehyde derivative negative charge located either aldehyde group (acyl...
The Pictet–Spengler (PS) cyclizations of β3-hTrp derivatives as arylethylamine substrates were performed with L-α-amino and D-α-amino aldehydes carbonyl components. During the PS reaction, a new stereogenic center was created, mixture cis/trans 1,3-disubstituted 1,2,3,4-tetrahydro-β-carbolines obtained. ratio diastereomers depends on centre used amino aldehyde size substituents. It confirmed by 1H 2D NMR (ROESY) spectra. conformations cyclic products studied ROESY Products condensation after...
Abstract. Aliphatic alcohols (AAs), including terpenoic (TAs), are ubiquitous in the atmosphere due to their widespread emissions from natural and man-made sources. Hydroxyl radical (OH) is most important atmospheric oxidant both aqueous gas phases. Consequently, oxidation of TAs by OH inside clouds fogs a potential source secondary organic aerosols (aqSOAs). However, kinetic data, necessary estimate time scales such reactions limited. Here, bimolecular rate coefficients (kOHaq) for...
The invention pertains to novel hybrid peptidomimetics, pharmaceutical composition comprising thereof and use in the treatment of neuropathic pain. peptidomimetics according are comprised two components: an opioid receptor agonist (OP) a MC4 antagonist, connected by linker. compounds such structure will allow activate receptors with simultaneous blocking melanocortin type 4 (MC4), which results enhancing efficiency therapy