A5023176309- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- Biofuel production and bioconversion
- Plant Gene Expression Analysis
- Microbial Natural Products and Biosynthesis
- Monoclonal and Polyclonal Antibodies Research
- Transgenic Plants and Applications
- Bacterial Genetics and Biotechnology
- Fermentation and Sensory Analysis
- Biochemical and biochemical processes
- Gene Regulatory Network Analysis
- Enzyme Catalysis and Immobilization
- Viral Infectious Diseases and Gene Expression in Insects
- Bacteriophages and microbial interactions
Chalmers University of Technology
2021-2023
University of Technology
2021
(2S)-Naringenin is a key precursor for biosynthesis of various high-value flavonoids and possesses variety nutritional pharmaceutical properties on human health. Systematic optimization approaches have been employed to improve (2S)-naringenin production in different microbial hosts. However, very few studies focused the spatiotemporal distribution related pathway intermediate p-coumaric acid, which an important factor efficient production. Here, we first optimized biosynthetic by alleviating...
Abstract Background Two important flavonoids, kaempferol and quercetin possess remarkably potent biological impacts on human health. However, their structural complexity low abundance in nature make both bulk chemical synthesis extraction from native plants difficult. Therefore microbial production via heterologous expression of plant enzymes can be a safe sustainable route for production. Despite several attempts reported hosts, the levels still stay far behind compared to many other...
The development of a cost-competitive bioprocess requires that the cell factory converts feedstock into product interest at high rates and yields. However, microbial factories are exposed to variety different stresses during fermentation process. These can be derived from feedstocks, metabolism, or industrial production processes, limiting capacity diminishing competitiveness. Improving stress tolerance robustness allows for more efficient ultimately makes process economically viable. This...
The flavonoid pinocembrin and its derivatives have gained increasing interest for their benefits on human health. While can be produced in engineered Saccharomyces cerevisiae, yields remain low. Here, we describe novel strategies improved de novo biosynthesis of from glucose based overcoming existing limitations S. cerevisiae. First, identified cinnamic acid as an inhibitor synthesis. Second, by screening more efficient enzymes optimizing the expression downstream genes, reduced...
Abstract Background Affibody molecules are synthetic peptides with a variety of therapeutic and diagnostic applications. To date, have mainly been produced by the bacterial production host Escherichia coli. There is an interest in exploring alternative hosts to identify potential improvements terms yield, ease purification advantages. In this study, we evaluated feasibility Saccharomyces cerevisiae as chassis for group proteins. Results We examined three different S. found that these were...
Yarrowia lipolytica has been explored as a potential production host for flavonoid synthesis due to its high tolerance aromatic acids and ability supply malonyl-CoA. However, little is known about consume the precursors cinnamic p-coumaric acid. In this study, we demonstrate that Y. can these through multiple pathways are partially dependent on cultivation medium. By monitoring acid concentrations over time, found converted We identified proteins with trans-cinnamate 4-monooxygenase activity...
Abstract The transition towards a bioeconomy requires the microbial production of various products from renewable resources such as lignocellulosic hydrolysate. Yarrowia lipolytica has been explored potential host for flavonoid synthesis due to its high tolerance aromatic acids and ability supply malonyl-CoA. However, little is known about consume precursors cinnamic p -coumaric acid. In this study, we demonstrate that Y. can these through multiple pathways are partially dependent on...
Abstract (2 S )-Naringenin is a key precursor for biosynthesis of various high-value flavonoids and possesses variety nutritional pharmaceutical properties on human health. Systematic optimization approaches have been employed to improve )-naringenin production in different microbial hosts. However, very few studies focused the spatiotemporal distribution related pathway intermediate p -coumaric acid, which an important factor efficient production. Here, we show that fine-turning acid...
Abstract Background Affibody molecules are synthetic peptides with a variety of therapeutic and diagnostic applications. To date, have mainly been produced by the bacterial production host Escherichia coli. There is an interest in exploring alternative hosts to address if improvements terms yield, ease purification advantages can be identified. In this study, we evaluated feasibility Saccharomyces cerevisiae as chassis for group proteins. Results We examined three different S. found that...