A5101690939- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Plant biochemistry and biosynthesis
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Fermentation and Sensory Analysis
- Plant Gene Expression Analysis
- Cassava research and cyanide
- Drug-Induced Hepatotoxicity and Protection
- RNA and protein synthesis mechanisms
- Chronic Kidney Disease and Diabetes
- Bacteriophages and microbial interactions
- Traditional Chinese Medicine Analysis
- Alcohol Consumption and Health Effects
- CRISPR and Genetic Engineering
- Antioxidant Activity and Oxidative Stress
- Liver Disease Diagnosis and Treatment
- Bioactive Natural Diterpenoids Research
- Microbial Metabolism and Applications
- Phytochemical Studies and Bioactivities
- Bacterial Genetics and Biotechnology
- Neurological Disease Mechanisms and Treatments
- Biochemical and biochemical processes
- Phytochemistry and Bioactivity Studies
- Occupational exposure and asthma
Chalmers University of Technology
2017-2023
First Affiliated Hospital of Bengbu Medical College
2023
Baotou Medical College
2017-2022
Inner Mongolia University of Science and Technology
2021
Tsinghua University
2015-2020
Nankai University
2010-2019
Beijing University of Chemical Technology
2015
Ministry of Education of the People's Republic of China
2010
Luohe Medical College
2008
Abstract The production of bioactive plant compounds using microbial hosts is considered a safe, cost-competitive and scalable approach to their production. However, some like aromatic amino acid (AAA)-derived chemicals, remains an outstanding metabolic engineering challenge. Here we present the construction Saccharomyces cerevisiae platform strain able produce high levels p -coumaric acid, AAA-derived precursor for many commercially valuable chemicals. This achieved through AAA biosynthesis...
Abstract Isoflavonoids comprise a class of plant natural products with great nutraceutical, pharmaceutical and agricultural significance. Their low abundance in nature structural complexity however hampers access to these phytochemicals through traditional crop-based manufacturing or chemical synthesis. Microbial bioproduction therefore represents an attractive alternative. Here, we engineer the metabolism Saccharomyces cerevisiae become platform for efficient production daidzein, core...
Abstract Production of chemicals and biofuels through microbial fermentation is an economical sustainable alternative for traditional chemical synthesis. Here we present the construction a Saccharomyces cerevisiae platform strain high-level production very-long-chain fatty acid (VLCFA)-derived chemicals. Through rewiring native elongation system implementing heterologous Mycobacteria FAS I system, establish increased biosynthesis VLCFAs in S. cerevisiae. can be selectively modified towards...
(2S)-Naringenin is a key precursor for biosynthesis of various high-value flavonoids and possesses variety nutritional pharmaceutical properties on human health. Systematic optimization approaches have been employed to improve (2S)-naringenin production in different microbial hosts. However, very few studies focused the spatiotemporal distribution related pathway intermediate p-coumaric acid, which an important factor efficient production. Here, we first optimized biosynthetic by alleviating...
The cellular machinery that supports protein synthesis and secretion lies at the foundation of cell factory-centered production. Due to complexity such machinery, challenge in generating a superior factory is fully exploit production potential by finding beneficial targets for optimized strains, which ideally could be used improved other proteins. We focused on an approach yeast Saccharomyces cerevisiae allows attenuation gene expression, using RNAi combined with high-throughput microfluidic...
Cell proliferation requires the integration of catabolic processes to provide energy, redox power and biosynthetic precursors. Here we show how combination rational design, metabolic rewiring recombinant expression enables establishment a decarboxylation cycle in yeast cytoplasm. This can support growth by supplying energy increased provision NADPH or NADH cytosol, which production highly reduced chemicals such as glycerol, succinate free fatty acids. With this approach, acid yield reached...
Caffeic acid is a plant phenolic compound possessing extensive pharmacological activities. Here, we identified that p-coumaric 3-hydroxylase from Arabidopsis thaliana was capable of hydroxylating to form caffeic in Saccharomyces cerevisiae. Then, introduced combined biosynthetic pathway into S. cerevisiae and obtained 0.183 mg L–1 glucose. Next improved the tyrosine biosynthesis by blocking flux aromatic alcohols eliminating tyrosine-induced feedback inhibition resulting production 2.780...
Context: Terrestrosin D (TED), from Tribulus terrestris L. (Zygophyllaceae), exhibits anti-tumour and anti-inflammatory activities. However, its effects on bleomycin (BLM)-induced pulmonary inflammation the subsequent fibrotic changes remain unclear.Objective: To examine anti-fibrotic of TED against BLM in murine tissues.Materials methods: Male SPF mice received saline (control), (10 mg/kg), (2.5 or mg/kg) + group. was administered as a single intranasal inoculation, intraperitoneally once...
In biotechnological industry, increased expression cassette stability and copy number serve as important means of maintaining consistently high production levels heterologous proteins in Saccharomyces cerevisiae. this study, we combined δ sequences for site-specific integration with TPI1 gene from Schizosaccharomyces pombe (POT1) a selection marker to realize high-copy stable S. With the newly developed POT1 platform, 32-copy enhanced green fluorescent protein (EGFP) was obtained single...
The development of a high-throughput and automatic system by combining gene amplification with MCE for HPV16 E6/E7 mRNA screening is reported.
Failure of glioblastoma (GBM) therapy is often ascribed to different types stem-like cell (GSLC) niche; in particular, a hypoxic perivascular niche (HPVN) involved GBM progression. However, the cells responsible for HPVNs remain unclear.Immunostaining was performed determine HPVNs. A chamber and 3-dimensional (3D) microfluidic chips were designed simulate HPVN based on pathological features GBM. The phenotype GSLCs evaluated by fluorescence scanning real time proliferation apoptotic assays....
The seeds of Amygdalus mongolica contain various constituents including flavonoids and vitamin E, which are known to exert antioxidant effects. However, the safety oil extract this compound is not fully known. aim study was determine physicochemical properties A. oil, identify subsequently assess effectiveness utilizing seed in hyperlipidemia as an agent. In particular, toxicity were examined with emphasis on effects blood lipids level serum lipid peroxidation using a rat model. Treatment 20...
Inhalation of silicon dioxide (SD) results in pulmonary inflammatory responses and fibrosis. Isorhynchophylline (Isorhy) is the main alkaloid traditional Chinese herb Tripterygium wilfordii, which reported to have anti-inflammatory activities nervous system. However, effects Isorhy on SD-induced inflammation fibrosis mice are unknown. Male were exposed a single dose SD (2.5 mg/kg, intranasal inoculation) induce (PF). The woken up immediately treated with (20 intraperitoneal injection) for 14...
Total Panax notoginseng saponin (TPNS) is the main bioactivity compound derived from roots and rhizomes of (Burk.) F.H. Chen. The aim this study was to investigate effectiveness TPNS in treating vascular neointimal hyperplasia rats its mechanisms. Male Sprague-Dawley were randomly divided into five groups, sham (control), injury, low, medium, high dose (5, 10, 20 mg/kg). An vivo 2F Fogarty balloon-induced carotid artery injury model established rats. significantly dose-dependently reduced...
Purpose To explore fecal immune-related proteins that can be used for colorectal cancer (CRC) diagnosis. Patients and methods Three independent cohorts were in present study. In the discovery cohort, which included 14 CRC patients 6 healthy controls (HCs), label-free proteomics was applied to identify stool could Exploring potential links between gut microbes by 16S rRNA sequencing. The abundance of immune-associated verified ELISA two validation a biomarker panel constructed cohort I 192...
1. To investigate Genkwa Flos hepatotoxicity, a cell metabolomics strategy combined with serum pharmacology was performed on human HL-7702 liver cells in this study. 2. Firstly, viability and biochemical indicators were determined the morphology observed to confirm injury develop hepatotoxicity model. Then, help of based UPLC-MS, group samples completely separated from blank score plots seven upregulated as well two down-regulated putative biomarkers loading plot identified confirmed....