A5103261574- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Parkinson's Disease Mechanisms and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Fungal and yeast genetics research
- Neurological disorders and treatments
- Botulinum Toxin and Related Neurological Disorders
- Voice and Speech Disorders
- Biofuel production and bioconversion
- Plant biochemistry and biosynthesis
- Nuclear Receptors and Signaling
- Asthma and respiratory diseases
- Plant Gene Expression Analysis
- Transgenic Plants and Applications
- Fermentation and Sensory Analysis
- Dermatology and Skin Diseases
- Glioma Diagnosis and Treatment
- Alzheimer's disease research and treatments
- Biochemical and biochemical processes
- Contact Dermatitis and Allergies
- Viral gastroenteritis research and epidemiology
- RNA regulation and disease
- Food Quality and Safety Studies
- Food Safety and Hygiene
- Aquaculture Nutrition and Growth
Yangzhou University
2024
Ministry of Agriculture and Rural Affairs
2024
Hangzhou Dianzi University
2024
Zhejiang Hospital
2018-2023
Zhejiang University
2010-2023
Nankai University
2018-2022
Beijing Technology and Business University
2019-2022
Zhejiang University of Technology
2022
Hainan Medical University
2021
State Key Laboratory of Medicinal Chemical Biology
2021
Food allergy affects approximately 6-8% of children, and increasing in prevalence. Some children naturally outgrow their food without intervention, but the mechanisms by which this occurs remain poorly understood. We sought to investigate role regulatory T cells development acquired tolerance.Fifty-eight (1-18 years) with either egg or peanut allergy, recent acquisition natural tolerance peanut, no were studied. Peripheral blood mononuclear (PBMC) from these groups stimulated relevant...
As a traditional ethanol-producing microorganism, Saccharomyces cerevisiae is an ideal host for consolidated bioprocessing. However, expression of heterologous cellulase increases the metabolic burden in yeast, which results low activity and poor cellulose degradation efficiency. In this study, cellulase-expressing yeast strains that could efficiently degrade different cellulosic substrates were created by optimizing ratios through POT1-mediated δ-integration strategy. Metabolic engineering...
Caffeic acid is a plant phenolic compound possessing extensive pharmacological activities. Here, we identified that p-coumaric 3-hydroxylase from Arabidopsis thaliana was capable of hydroxylating to form caffeic in Saccharomyces cerevisiae. Then, introduced combined biosynthetic pathway into S. cerevisiae and obtained 0.183 mg L–1 glucose. Next improved the tyrosine biosynthesis by blocking flux aromatic alcohols eliminating tyrosine-induced feedback inhibition resulting production 2.780...
Mutations in the parkin gene have been significantly linked to autosomal recessive juvenile parkinsonism (ARJP). In addition its association with ARJP, loss of heterozygosity has found several types malignant tumors, including ovarian, breast, and hepatocellular tumors. Abnormal methylation promoter was observed patients acute lymphoblastic leukemia chronic myelogenous lymphoid blast crisis. We hypothesized that hypermethylation might reduce expression parkin, which would contribute...
In recent years, many studies have been conducted on the expression of multiple aromatic compounds by Saccharomyces cerevisiae. The concentration l-tyrosine, as a precursor such valuable compounds, is crucial for biosynthesis metabolites. this study, novel function HTZ1 was found to be related tyrosine biosynthesis, which has not yet reported. Knockout gene could significantly improve ability yeast cells synthesize tyrosine, and its p-coumaric acid (p-CA) titer approximately 3.9-fold higher...
Aromatic compounds, such as p-coumaric acid (p-CA) and caffeic acid, are secondary metabolites of various plants, widely used in diet industry for their biological activities. In addition to expensive unsustainable methods plant extraction chemical synthesis, the strategy heterologous synthesis aromatic compounds microorganisms has received much attention. As most abundant renewable resource world, lignocellulose is an economical environmentally friendly alternative edible, high-cost carbon...
Caffeic acid, a plant-sourced phenolic compound, has variety of biological activities, such as antioxidant and antimicrobial properties. The caffeic acid biosynthetic pathway was initially constructed in S. cerevisiae, using codon-optimized TAL (coTAL, encoding tyrosine ammonia lyase) from Rhodobacter capsulatus, coC3H (encoding p-coumaric 3-hydroxylase) coCPR1 cytochrome P450 reductase 1) Arabidopsis thaliana 2 μ multi-copy plasmids to produce glucose. Then, integrated expression coTAL via...
The present study aimed to examine how the long non‑coding RNA (lncRNA) RP11‑543N12.1 interacted with microRNA (miR)‑324‑3p modify microglials (MIs)‑induced neuroblastoma cell apoptosis, which may pose benefits treatment of Alzhemier's disease (AD). model AD was established by treating SH‑SY5Y cells amyloid β (Aβ)25‑35, and MI were acquired using primary culture technology. lncRNAs that differentially expressed between control screened through a microarray assay confirmed via polymerase...
The chemokine monocyte chemoattractant protein-1 (MCP-1) is involved in the pathogenesis of Alzheimer's disease (AD). This study aimed to investigate whether urinary MCP-1 can distinguish patients with AD, amnestic mild cognitive impairment (aMCI) and cognitively normal (CN) subjects.A total 754 participants, including 97 50 aMCI 84 age- sex-matched CN controls as well a cohort 523 subjects different ages, were enrolled from five hospitals located areas China. Urinary levels determined using...
By using elevated level of phosphatase and reductase in lung cancer cells, we designed a tandem molecular self-assembling prodrug that could selectively improve the therapeutic efficacy HCPT against by two orders magnitude.
Deep-learning methods (especially convolutional neural networks) using magnetic resonance imaging (MRI) data have been successfully applied to computer-aided diagnosis of Parkinson's Disease (PD). Early detection and prior care may help patients improve their quality life, although this neurodegenerative disease has no known cure. In study, we propose a FResnet18 model classify MRI images PD Health Control (HC) by fusing image texture features with deep features. First, Local Binary Pattern...
To overcome the defective unstable production of p-coumaric acid (p-CA) using episomal plasmids and simultaneously achieve genetic stability high-copy integration in Saccharomyces cerevisiae.Two-micron were used to obtain high titres p-CA, but p-CA was decreased significantly a nonselective medium after 72 h. defect during fermentation, delta with triosephosphate isomerase gene from Schizosaccharomyces pombe (POT1) employed as selection marker integrate heterologous synthesis cassette,...
Background Uncontrollable body movements are typical symptoms of Parkinson's disease (PD), which results in inconsistent findings regarding resting‐state functional connectivity (rsFC) networks, especially for group difference clusters. Systematically identifying the motion‐associated data was highly demanded. Purpose To determine censoring criteria using a quantitative cross validation‐based (CVDC) method and to improve detection rsFC deficits PD. Study Type Prospective. Subjects Forty‐one...