Phosphate is the second most essential nutrient for plant growth and development.Although soil contains abundant phosphorus, only 0.1% used by roots due to significant amount of calcium carbonate (CaCO3) present in calcareous soil, which limits availability P reacting ions with phosphate ions.It leads alkaline reactions generating insoluble compounds such as tricalcium phosphate.Here we study boost solubilization PSMs an alternate strategy.Twenty-one bacterial isolates were selected from two...

The virus known as human immunodeficiency (HIV) targets immune system cells, increasing a person's susceptibility tovarious maladies and diseases. (MEHMOOD, et al. [1,2]) is member of the retroviral family Orthoretrovirinae belongs to genus Lentivirus. (Mozhi [3]). HIV divided into types 1 2 according genetic attributes variations in viral proteins (HIV-1, HIV-2). HIV-1 three classes: group M (main), O (outlier), N. (Dubey [4]) far more varied group, Group M, made up nine subtypes (A-D, F-H,...

MicroRNAs are small non-coding RNA molecules that produced in a cell endogenously. They made up of 18 to 26 nucleotides strength. Due their evolutionary conserved nature, most the miRNAs provide logical basis for prediction novel and clusters plants such as sunflowers related Asteraceae family. In addition, they participate different biological processes plants, including signaling metabolism, development, growth, tolerance (biotic abiotic) stresses. this study profiling, conservation...

A technique called phytoremediation makes use of a plant’s root system to draw contaminants, primarily heavy metals (zn, cr, and ni), from the surrounding rhizosphere. These are end result human actions such pesticides, waste byproducts, cosmetics, pharmaceuticals. This analysis focuses on soil contamination removal since prolonged exposure can cause respiratory illnesses, cardiovascular disorders, cancer liver, lungs, kidneys, among other problems. In order immobilise change their harmful...

Glioblastoma multiforme (GBM) is one of the most aggressive cancers with a low overall survival rate. The treatment GBM challenging due to presence blood–brain barrier (BBB), which hinders drug delivery. Invasive procedures alone are not effective at completely removing such tumors. Hence, identifying crucial pathways and biomarkers for prime importance. We conducted this study identify associated GBM. used Cancer Genome Atlas (TCGA) genomic dataset differentially expressed genes (DEGs)....

The corona name derived from their crown like spike proteins attach with cell receptors. It belongs to coronaviradae family and nideovirales order, envelop virus, size range 65-125 ​nm positive single standard RNA between 26.4 31.7 ​kb contain 7096 amino acid. There are four subtypes that have been detected these alpha, beta, gamma delta.The 267 covid-19 blood nasopharyngeal samples were collected Multan region. extraction run the PCR. use for clinical tests, Lactate dehydrogenase, serum...

SARS (COV-2) was recognized in 31/December/2019 Wuhan city the Hubei Provence of China. The omicron variant COVID-19 reported November 2021. Omicron can have many earlier documented mutations other concern (VOC) is including 32 spike like protein, it too compared 16 mutations. has been divided into four linages BA.1, BA.2, BA.3, B.1.1.529. BA.1 which could destroyed our immune system and vaccine are not highly effective against BA.2.

Cannabis sativa is a significant crop of the family (cannabaceae).Interest has been resurgent in usage cannabis plant for medical purposes, industrial fibre, seed oil, food, and recreation.Auxin cytokinins act antagonistically with each other which promote shoot branching inhibit root branching, while auxin inhibits promotes branching. Young leaves petioles were used inoculated MS medium various combinations. Murashige Skoog (MS) explant developed additions 25µl-450µl 2, 4 D, Kinetin,...

Research in Medical & Engineering Sciences Genomic Analysis of BRCA2, and a Comparative Phylogenetic BRCA2 Humans along Different Species: A Candidate for Breast Cancer Nimra Yaseen1, Muhammad Aetesam Nasir2, Hanzala Inam2, Izzah Mudassar2, Mamoona Arshad2, Ifrah Saroosh2, Rubace Fatima Mirza3, Syeda Zuha Naqvi4 Waqar Mazhar1* 1Department Bioinformatics Biotechnology, Government College University, Faisalabad, Pakistan 2Department medicine surgery, Hitec-institute medical sciences taxila...

Background: The human immunodeficiency virus is categorised in the genus Lentivirus, subfamily Orthoretrovirinae of Retroviridae family. Methodology: 40 specimens total were included research to conduct whole experimentation. viral RNA extraction was performed by using Qiagen kit. After RNA, cDNA synthesized. One step rt-PCR kit used for amplification pair primers forward (A) & reverse primer (D) 10uM/ug. Sequencing and capillary electrophoresis performed. phylogenetic tree HIV done...

SARS (COV-2) was recognized in 31/December/2019 Wuhan city the Hubei Provence of China. The omicron variant COVID-19 reported November 2021. Omicron can have many earlier documented mutations other concern (VOC) is including 32 spike like protein, it too compared 16 mutations. has been divided into four linages BA.1, BA.2, BA.3, B.1.1.529. BA.1 which could destroy our immune system and vaccine are not highly effective against BA.2.

Microorganisms are critical for the survival of life on Earth, but we know very little about vast majority ofmicrobes. We studied culturable soybean root, soil, and nodules microbiome here. Thirty bacterial sampleswere isolated from different localities crops, including nodules, to assess their abilityfor plant growth promotion. To evaluate richness diversity in root performed mostprobable number (MPN) test. Seven isolates were able produce cellulase, which JSW1 andJSW6 most efficient with...

Introduction: The HBV virus has its enveloped protein that surrounds nucleic acid for protection. Hepatitis B DNA belongs to the Hepadnoviridae family and is similar retroviruses. Globally, infected people approximately 2 billion in world, about 350 million were chronic carriers. One deaths are caused by every year. Each year, 400,000 new cases reported Latin America. Among all over World, Pakistan was considered as one of largest viral hepatitis infection countries. Methodology: 975 samples...

Wounds are common external or internal damages to the body, usually caused by a physical, chemical, biological agents. There exists natural repair wound healing in that includes immunological responses i.e., inflammation and necrosis. Naturally, body repairs wounds itself, but period of is relatively slow. This process may further be delayed pathogens attack weaker immune response. heal biochemically, time can reduced applying different herbs like plant-based treatments. Sub-continent rich...

In recent years, stem cell therapy has become a very promising and complex scientific research topic. the body, cells have capacity to differentiate into wide variety of types. They are capable regenerate body system. Adult embryonic two primary categories cells. Through cell-replacement therapies, cell-based therapies can give opportunity replace lost, damaged, or old in order repair tissues organs. Additionally, using genetically altered as delivery systems holds enormous potential for...

Abstract In this research, the comparative analysis of heavy metals has been studied. An experiment was conducted to determine bioaccumulation in wheat as is staple cereal crop consumed food globally. Abiotic stress disturbs metabolic pathway plants, and cause enzymatic degradation, reduction photosynthesis, increased level ROS species. Comparative Cd, Pb, Cr, Cu, Ni analyzed with help Inductively Coupled plasma optical emission spectrometry (ICP-OES). The results show that a maximum value...

Abstract Introduction:The gut microbiota plays a significant role in fish health and pathogenicity. L.rohita is well known farmed it also culture with other major carps.Methodology:The present study was conducted to determine the molecular morphological identification of collected from hatchery Faisalabad. Isolation bacteria carried out through techniques. The bacterial isolates determined by gram staining, biochemical tests TSI, MR-VP test. done 16S rRNA techniques.Results:The results...

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