A5084889294- Olfactory and Sensory Function Studies
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Advanced Chemical Sensor Technologies
- Neuroscience and Neural Engineering
- Insect Pheromone Research and Control
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Electrochemical Analysis and Applications
- Memory and Neural Mechanisms
- Analytical Chemistry and Sensors
- Photochromic and Fluorescence Chemistry
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Circadian rhythm and melatonin
- Retinal Development and Disorders
- Insect Utilization and Effects
- Sparse and Compressive Sensing Techniques
- Crustacean biology and ecology
- Blind Source Separation Techniques
- Ion channel regulation and function
- Advanced Fluorescence Microscopy Techniques
- Cellular transport and secretion
- Neuroinflammation and Neurodegeneration Mechanisms
University of Utah
2013-2025
Duke University
1989-2021
Lehigh University
2021
Pennsylvania State University
2021
Duke Medical Center
2021
Institute of Neurobiology
2018
Marine Biological Laboratory
1998-2015
Boston University
2004-2014
Institute of Social Work
2011
Yale University
1999-2004
Odorants are first represented in the brain by distributed patterns of activity olfactory bulb (OB). Although neurons downstream sensory inputs respond to odorants with temporally structured activity, glomeruli typically described as static maps. Here, we imaged temporal dynamics receptor neuron input OB a calcium-sensitive dye nerve terminals anesthetized mice. We found that diverse, glomerulus- and odorant-dependent present even at this initial stage. Instantaneous spatial changed both...
Sniffing, a rhythmic inhalation and exhalation of air through the nose, is behavior thought to play critical role in shaping how odor information represented processed by nervous system. Although mouse has become prominent model for studying olfaction, little known about sniffing mice. Here, we characterized measuring intranasal pressure transients behaving Sniffing was monitored during unstructured exploratory performance 3 commonly used olfactory paradigms: habituation/dishabituation task,...
To gain insight into which parameters of neural activity are important in shaping the perception odors, we combined a behavioral measure odor with optical imaging representations at level receptor neuron input to rat olfactory bulb. Instead typical test an animal's ability discriminate two familiar odorants by exhibiting operant response, used spontaneously expressed response novel odorant—exploratory sniffing—as perception. This assay allowed us speed rats perform spontaneous...
The dynamics of sensory input to the nervous system play a critical role in shaping higher-level processing. In olfactory system, from receptor neurons (ORNs) are poorly characterized and depend on multiple factors, including respiration-driven airflow through nasal cavity, odorant sorption kinetics, receptor-ligand interactions between receptor, electrophysiological properties ORNs. Here, we provide detailed characterization temporal organization ORN mammalian bulb (OB) during natural...
Understanding central processing requires precise monitoring of neural activity across populations identified neurons in the intact brain. In present study, we used recently optimized variants genetically encoded calcium sensor GCaMP (GCaMP3 and GCaMPG5G) to image among anatomically defined neuronal olfactory bulb (OB), including two types GABAergic interneurons (periglomerular [PG] short axon [SA] cells) OB output (mitral/tufted [MT] projecting piriform cortex. We first established that...
Neural activity underlying odor representations in the mammalian olfactory system is strongly patterned by respiratory behavior. These dynamics are central to many models of information processing. We have found previously that sensory inputs bulb change both their magnitude and temporal structure as a function sniff frequency. Here, we ask how frequency affects responses mitral/tufted (MT) cells—the principal output neurons. recorded from MT cells anesthetized rats while reproducing sniffs...
Cholinergic modulation of central circuits is associated with active sensation, attention, and learning, yet the neural temporal dynamics underlying cholinergic effects on sensory processing remain unclear. Understanding particular complicated by widespread projections neurons to telencephalic structures that themselves are highly interconnected. Here we examined how from basal forebrain olfactory bulb (OB) modulate output first stage in mouse system. By optogenetically activating their...
Tools enabling the manipulation of well defined neuronal subpopulations are critical for probing complex networks. Cre recombinase (Cre) mouse driver lines in combination with Cre-dependent expression proteins using viral vectors--in particular, recombinant adeno-associated vectors (rAAVs)--have emerged as a widely used platform achieving transgene specified neural populations. However, ability rAAVs to further specify subsets on basis their anatomical connectivity has been reported limited...
We measured the spatiotemporal aspects of odor-induced population response in turtle olfactory bulb using a voltage-sensitive dye, RH414, and 464-element photodiode array. In contrast with previous studies activity local field potential recordings, we distinguished four signals response. The one called DC covered almost entire area bulb; addition, three oscillations, named rostral, middle, caudal according to their locations, occurred over broad regions bulb. typical response, signal...
Presynaptic regulation of transmission at the first olfactory synapse was investigated by selectively imaging axon terminals receptor neurons in lobster lobe and turtle bulb. In both species, action potential propagation into after nerve stimulation measured using voltage-sensitive dyes. addition, turtle, calcium influx labeling with dextran-conjugated indicator lobster, application inhibitory transmitters GABA or histamine suppressed potentials terminals. The suppression blocked picrotoxin...
We investigated the cellular mechanism underlying presynaptic regulation of olfactory receptor neuron (ORN) input to mouse bulb using optical-imaging techniques that selectively report activity in ORN terminal. First, we loaded ORNs with calcium-sensitive dye and imaged stimulus-evoked calcium influx a slice preparation. Single nerve shocks evoked rapid fluorescence increases were largely blocked by N-type channel blocker omega-conotoxin GVIA. Paired revealed long-lasting suppression...
Many mammals display brief bouts of high-frequency (4-10 Hz) sniffing when sampling odors. Given this, is thought to play an important role in odor information processing. Here, we asked what rapid behavior plays coding and discrimination by monitoring during performance tasks under different paradigms across levels difficulty imaging olfactory receptor neuron (ORN) input the bulb (OB) behavior. To eliminate confounds locomotion object approach, all experiments were performed head-fixed...
Processing of sensory information is substantially shaped by centrifugal, or feedback, projections from higher cortical areas, yet the functional properties these are poorly characterized. Here, we used genetically-encoded calcium sensors (GCaMPs) to functionally image activation centrifugal targeting olfactory bulb (OB). The OB receives massive input areas but there has been as no characterization their activity in vivo. We focused on anterior nucleus (AON), a major source feedback OB....
Serotonergic neurons in the brainstem raphe nuclei densely innervate olfactory bulb (OB), where they can modulate initial representation and processing of information. modulation sensory responses among defined OB cell types is poorly characterized vivo Here, we used cell-type-specific expression optical reporters to visualize how stimulation alters two classes GABAergic mouse glomerular layer, periglomerular (PG) short axon (SA) cells, as well mitral/tufted (MT) cells carrying output...
In olfactory systems, convergence of sensory neurons onto glomeruli generates a map odorant receptor identity. How glomerular maps relate to space remains unclear. We sought better characterize this relationship in the mouse system by defining terms odorants which they are most sensitive. Using high-throughput delivery and ultrasensitive imaging inputs, we imaged responses 185 presented at concentrations determined activate only one or few across dorsal bulb. The resulting datasets defined...
Glomeruli in the olfactory bulb are anatomically discrete modules receiving input from idiotypic sensory neurons. To examine functional organization of inputs to individual glomeruli, we loaded neurons with a Ca 2+ indicator and measured odorant-evoked presynaptic signals within single glomeruli by using two-photon microscopy anaesthetized mice. Odorants evoked patterns throughout neuropil glomerulus. Across occurred equal probability all glomerular regions. Within pattern intraglomerular...