A5081807421- Reproductive Biology and Fertility
- Assisted Reproductive Technology and Twin Pregnancy
- Ovarian function and disorders
- Sperm and Testicular Function
- Reproductive Health and Technologies
- Prenatal Screening and Diagnostics
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Protease and Inhibitor Mechanisms
- Immune Cell Function and Interaction
- Testicular diseases and treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ectopic Pregnancy Diagnosis and Management
- Pluripotent Stem Cells Research
- Blood Coagulation and Thrombosis Mechanisms
- Estrogen and related hormone effects
- Chromosomal and Genetic Variations
- Endometriosis Research and Treatment
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Renal and related cancers
- Genetic and phenotypic traits in livestock
- Cancer therapeutics and mechanisms
- CAR-T cell therapy research
Soroka Medical Center
2013-2024
Ben-Gurion University of the Negev
2014-2024
University Medical Center
1999-2024
Hadassah Medical Center
2023
Genetics and IVF Institute
2006
<h3>Background</h3> Azoospermia is diagnosed when sperm cells are completely absent in the ejaculate even after centrifugation. It identified approximately 1% of all men and 10%–20% infertile males. Non-obstructive azoospermia (NOA) characterised by absence due to either a Sertoli cell-only pattern, maturation arrest, hypospermatogenesis or mixed patterns. NOA severe form male infertility, with limited treatment options low fertility success rates. In majority patients, cause for not known...
Can a machine-learning-based model trained in clinical and biological variables support the prediction of presence or absence sperm testicular biopsy non-obstructive azoospermia (NOA) patients?Our machine-learning was able to accurately predict (AUC 0.8) spermatozoa patients with NOA.Patients NOA can conceive their own gametes using ICSI combination successful extraction (TESE). Testicular retrieval is up 50% men NOA. However, best our knowledge, there no existing that success TESE....
What is the clinical value of Day 7 blastocysts?Ending embryo culture at 144 hours post-insemination (h.p.i.; i.e. 6 days) would involve 7.3% and 4.4% relative reductions in number patients obtaining euploid blastocysts live birth(s) (LBs), respectively.Many studies showed that are clinically valuable, although less competent than faster-growing embryos. Nevertheless, a large variability exists in: (i) definition 'Day 7'; (ii) criteria to embryos 7; (iii) setting; (iv) local regulation;...
Abstract STUDY QUESTION What are the factors associated with human blastocyst spontaneous collapse and consequences of this event? SUMMARY ANSWER Approximately 50% blastocysts collapsed, especially when non-viable, morphologically poor and/or aneuploid. WHAT IS KNOWN ALREADY Time-lapse microscopy (TLM) is a powerful tool to observe preimplantation development dynamics. Lately, artificial intelligence (AI) has been harnessed automate standardize such observations. Here, we adopted AI...
In in vitro fertilization (IVF) treatments, early identification of embryos with high implantation potential is required for shortening time to pregnancy while avoiding clinical complications the newborn and mother caused by multiple pregnancies. Current classification tools are based on morphological morphokinetic parameters that manually annotated using time‐lapse video files. However, manual annotation introduces interobserver intraobserver variability provides a discrete representation...
Abstract In vitro fertilization is typically associated with high failure rates per transfer, leading to an acute need for the identification of embryos developmental potential. Current methods are tailored specific times after fertilization, often require expert inspection, and have low predictive power. Automatic challenged by ambiguous labels, clinical heterogeneity, inability utilize multiple points. this work, we propose a novel method that trains classifier conditioned on time since...
First trimester miscarriage is a major concern in IVF-ET treatments, accounting for one out of nine clinical pregnancies and up to three recognized pregnancies. To develop machine learning classifier predicting the risk cleavage-stage embryos undergo first based on time-lapse images preimplantation development.
Our objective was to design an automated deep learning model that extracts the morphokinetic events of embryos were recorded by time-lapse incubators. Using annotation, we set out characterize temporal heterogeneity preimplantation development across a large number embryos.
Abstract Blastocyst selection is primarily based on morphological scoring systems and morphokinetic data. These methods involve subjective grading time-consuming techniques. Artificial intelligence allows for objective quick blastocyst selection. In this study, 608 blastocysts were selected transfer using morphokinetics Gardner criteria. Retrospectively, morphometric parameters of size, inner cell mass (ICM) ICM-to-blastocyst size ratio, ICM shape automatically measured by a semantic...
Oxidative stress is induced by reactive oxygen substances (ROS) that are known to affect male fertility. The aims of this study were prospectively investigate and characterize total antioxidant specifically polyphenols concentrations their relations sperm quality fertility treatment success. During infertility treatment, sixty-seven males recruited study. After separation the from semen sample, fluid samples antioxidants determined. Antioxidant concentration was significantly associated with...
The condensed state of the human sperm's chromatin is essential for compact structure spermatozoon head, which important fertilization process. enzymes DNA topoisomerases (topo I and topo II) are responsible topological in somatic cells. activities characterization mature sperm cells have not been previously investigated.Sperm were purified from semen healthy donors by standard procedures assays measuring activities, protein size sensitivity to inhibitors performed.Topo II relaxation present...
Embryo implantation plays a major role in embryogenesis and the outcome of pregnancy. Plasminogen activators (PAs) have been implicated mammalian fertilization, early stages development embryo implantation. As in-vitro developing embryos resulted lower rate than those developed in-vivo we assume that reduced PAs activity may be involved. In present work studied effect EGF on activity, quantity implantation.Zygotes were flushed from rat oviducts day one pregnancy grown R1ECM supplemented with...
Embryo implantation plays a major role in embryogenesis and the outcome of pregnancy. Plasminogen activators (PAs) have been implicated mammalian fertilization, early stages development embryo implantation. The invasion trophoblast cells into endometrium during process can be blocked by inhibitors serine proteases, illustrating these enzymes process. As vitro developing embryos resulted lower rate than those developed vivo we assume that reduced PAs activity may lead to it. There is hardly...