A5055152049- Photochromic and Fluorescence Chemistry
- Photoreceptor and optogenetics research
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Receptor Mechanisms and Signaling
- Advanced Chemical Sensor Technologies
- Retinal Development and Disorders
- Analytical Chemistry and Sensors
- Mass Spectrometry Techniques and Applications
- Nicotinic Acetylcholine Receptors Study
Ludwig-Maximilians-Universität München
2014-2015
Center for Integrated Protein Science Munich
2015
The covalent attachment of synthetic photoswitches is a general approach to impart light sensitivity onto native receptors. It mimics the logic natural photoreceptors and significantly expands reach optogenetics. Here we describe novel photoswitch design—the photoswitchable orthogonal remotely tethered ligand (PORTL)—that combines genetically encoded SNAP-tag with photochromic ligands connected benzylguanine via long flexible linker. We use method convert G protein-coupled receptor mGluR2,...
Photochromic blockers of voltage gated ion channels are powerful tools for the control neuronal systems with high spatial and temporal precision. We now introduce fotocaine, a new type photochromic channel blocker based on long-lasting anesthetic fomocaine. Fotocaine is readily taken up by neurons in brain slices enables optical action potential firing switching between 350 450 nm light. It also provides an instructive example "azologization", that is, systematic conversion established drug...
Nicotinic acetylcholine receptors (nAChRs) are essential for cellular communication in higher organisms. Even though a vast pharmacological toolset to study cholinergic systems has been developed, control of endogenous neuronal nAChRs with high spatiotemporal precision lacking. To address this issue, we have generated photoswitchable nAChR agonists and re-evaluated the known photochromic ligand, BisQ. Using electrophysiology, found that one our new compounds, AzoCholine, is an excellent...
Nicotinic and muscarinic acetylcholine receptors (n/mAChRs) play a prominent role in shaping excitatory postsynaptic potentials intracellular signaling pathways. To study these systems, vast pharmacological toolset has been developed over the last decades. However, possibility to reversibly control receptor activation selectively with high spatiotemporal precision lacking. address this issue, two novel photochromic ligands (PCLs) have generated: AzoCholine target nicotinic AChRs AzoCarbachol...