A5103120978- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Toxin Mechanisms and Immunotoxins
- History and advancements in chemistry
- Galectins and Cancer Biology
- Transgenic Plants and Applications
- Monoclonal and Polyclonal Antibodies Research
- Polysaccharides and Plant Cell Walls
- African Botany and Ecology Studies
- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Biochemical and Structural Characterization
- Philosophy, Science, and History
- Plant biochemistry and biosynthesis
- Cultural Heritage Materials Analysis
- Cancer Research and Treatments
- Legume Nitrogen Fixing Symbiosis
- Pharmaceutical studies and practices
- Pancreatic function and diabetes
- German Literature and Culture Studies
- Dermatological and Skeletal Disorders
- Bladder and Urothelial Cancer Treatments
- Enzyme Structure and Function
- Liver physiology and pathology
- Photosynthetic Processes and Mechanisms
Université de Lille
1988-2016
Centre National de la Recherche Scientifique
1995-2014
École Nationale Supérieure de Chimie de Lille
1986-2014
Laboratoire de Chimie
1990-2009
Université des Sciences, de Technologie et de Médecine
2008
Unité de Glycobiologie Structurale et Fonctionnelle
2004-2006
Université de Reims Champagne-Ardenne
1999
Universidad Autónoma del Estado de Morelos
1997
Instituto Nacional de Enfermedades Respiratorias
1997
Université d'Artois
1988-1991
Glycopeptides and oligosaccharides of either the N ‐acetyllactosaminic or oligomannosidic type derived from glycoproteins containing ‐glycosylamine linkage were used to define specificity different lectins (concanavalin A, Lens culinaris agglutinin, Vicia faba Pisum sativum Ricinus communis agglutinins, soybean wheat germ Solanum tuberosum Datura stramonium Lotus tetragonolobus Ulex europeus agglutinin) by studying inhibition human red blood cell agglutination these structures. The results...
Circulating M antigen, specific for genus Schistosoma, was previously described in serum, urine, patients' milk, and serum urine of animals infected by S. mansoni. The antigen thermostable soluble trichloroacetic acid. It not hydrolyzed protease, ribonuclease, amylase, or neuraminidase but destroyed sodium metaperiodate. In the present study, we have purified ag using acid solubility, DEAE Sephadex, immunoadsorption. showed a neutral electric charge, m.w. heterogeneity, only stained periodic...
Periodate oxidation of soybean agglutinin, a glycoprotein lectin, resulted in destruction up to 5 out the 9 mannose residues present each its subunits (MW 30,000) without any loss hemagglutinating activity. The did, however, abolish interaction agglutinin with concanvalin A, as measured by quantitative precipitation. Reduction sodium [3-H]borohydride which 4 per subunit were oxidized, afforded radioactive product retained full activity and was indistinguishable from native lectin gel...
A lectin called Helianthus tuberosus agglutinin or Heltuba has been isolated from tubers of the Jerusalem artichoke, a typical representative Asteraceae family. is tetrameric protein composed four identical subunits 15.5 kDa and exhibits preferential specificity towards oligomannosides. Cloning corresponding cDNAs revealed that mature polypeptide comprises entire open reading frame cDNA suggesting primary translation product not processed cytosolic protein. Searches in databases sequence...
Legume lectins, despite high sequence homology, express diverse biological activities that vary in potency and efficacy. In studies reported here, the mannose-specific lectin from Cymbosema roseum (CRLI), which binds N-glycoproteins, shows both pro-inflammatory effects when administered by local injection anti-inflammatory systemic injection. Protein sequencing was obtained Tandem Mass Spectrometry crystal structure solved X-ray crystallography using a Synchrotron radiation source. Molecular...
Abstract HCA and HML represent lectins isolated from the red marine algae Hypnea cervicornis musciformis , respectively. Hemagglutination inhibition assays suggest that binds GalNAc/Gal substituted with a neutral sugar through 1–3, 1–4, or 1–2 linkages in O‐linked mucin‐type glycans, Fuc(α1–6)GlcNAc of N‐linked glycoproteins. The specificity includes epitopes recognized by HML, although glycoproteins inhibited distinctly HCA. agglutinating activity was GalNAc, highlighting different fine...
Parkia biglobosa (subfamily Mimosoideae), a typical tree from African savannas, possess seed lectin that was purified by combination of ammonium sulfate precipitation and affinity chromatography on Sephadex G‐100 column. The P . (PBL) strongly agglutinated rabbit erythrocytes, an effect inhibited d ‐mannose ‐glucose‐derived sugars, especially α‐methyl‐ ‐mannopyranoside N ‐acetyl‐ ‐glucosamine. hemagglutinating activity PBL maintained after incubation at wide range temperature pH also...
Glycoproteins and their derived glycopeptides have been used to define the specificity of Lathyrus ochrus lectin isolectins, by determining ability inhibit agglutination human erythrocytes induced or isolectins. This α‐D‐mannose/α‐D‐glucose‐specific possessess recognize a well‐defined saccharide sequence on bi‐antennary N ‐acetyllactosamine‐type glycan. For other Vicieae lectins, best inhibitor is glycopeptide from lactotransferrin with an α‐L‐fucose residue at C‐6 position...
Abstract Total cellular glycopeptides of 7 human uroepithelial cell lines that differ in the grade transformation (TGr) were analysed by gel filtration and affinity chromatography on immobilized lectins. The 4 are tumorigenic nude mice invasive vitro (TGr III) possess more highly branched, tri‐ tetraantennary N‐acetyllactosaminic glycans, with less biantennary glycans than 2 non‐tumorigenic, non‐invasive II) examined. only exception to this general pattern is third line, which classified as...
The carbohydrate-binding activity of the algal lectins from closely related red marine species Bryothamnion triquetrum (BTL) and seaforthii (BSL) was used to differentiate human colon carcinoma cell variants with respect their membrane glyco-receptors. These interacted cells tested in a dose-dependent manner. Moreover, fluorescence spectra both clearly differentiated as shown by FACS profiles. Furthermore, observed confocal microscopy, BTL BSL bound surface glycoproteins underwent intense...
The relation structure-activity of the Mimosoideae lectins Parkia platycephala (PPL) and biglobosa (PBL) was analyzed in this study. PBL solved by X-ray crystallography at a resolution 2.1 ÿ, crystal structure belonged to C2221 space group. Structural organization binding sites were also characterized. Specifically, monomer consists three β-prism domains tandemly arranged with each one presenting different carbohydrate recognition domain (CRD). PPL showed antinociceptive activity mouse model...
The asialoglycopeptides obtained from secretory immunoglobulins A human milk have been separated by gel filtration and affinity chromatography on Concanavalin A-Sepharose Lens culinaris agglutinin-Sepharose columns. Their structures determined sugar analysis, methylation studies including mass spectrometry 500-MHz 1H-NMR spectroscopy. glycans are of the biantennary N-acetyllactosamine type differing in their degree extension fucosyl-N-acetyllactosamine residues. overall glycopeptides as...
Parkia platycephala lectin 2 was purified from (Leguminosae, Mimosoideae) seeds by affinity chromatography and RP‐HPLC. Equilibrium sedimentation MS showed that is a nonglycosylated monomeric protein of molecular mass 29 407 ± 15 Da, which contains six cysteine residues engaged in the formation three intramolecular disulfide bonds. agglutinated rabbit erythrocytes, this activity specifically inhibited N ‐acetylglucosamine. In addition, hydrolyzed β(1–4) glycosidic bonds linking...