A5077089086- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- Natural product bioactivities and synthesis
- Protein Structure and Dynamics
- Autophagy in Disease and Therapy
- African Botany and Ecology Studies
- Escherichia coli research studies
- Invertebrate Immune Response Mechanisms
- Enzyme Production and Characterization
- Plant biochemistry and biosynthesis
- Biochemical and Structural Characterization
- RNA and protein synthesis mechanisms
- Polysaccharides and Plant Cell Walls
- Proteins in Food Systems
- Peanut Plant Research Studies
- Biochemical and Molecular Research
- Atherosclerosis and Cardiovascular Diseases
- Receptor Mechanisms and Signaling
- Ferroptosis and cancer prognosis
- Microbial Metabolism and Applications
- Complement system in diseases
- Pharmacological Effects of Natural Compounds
Universidade Federal do Ceará
2016-2025
The mechanisms behind Concanavalin A (ConA) circular permutation have been under investigation since 1985. Although a vast amount of information is available about this lectin and its applications, the exact purpose processing remains unclear. To shed light on this, study employed computer simulations to compare unprocessed ProConA with mature ConA. This approach aimed reveal importance post-translational modifications, especially how they affect stability carbohydrate-binding properties....
The genus Centrolobium comprises species of Neotropical trees with seeds that possess medicinal and bioactive applications. Lectins from this exhibit anti-inflammatory immunomodulatory effects, influencing the activation immune system. This study focuses on characterizing structure carbohydrate-binding properties lectin microchaete (CML) evaluating its potential against gliomas. in complex methyl-mannose-α1,3-mannose (MDM) was resolved using X-ray crystallography at 1.3 Å resolution,...
Gliomas, ranging from low-grade pilocytic astrocytomas to highly malignant glioblastomas, are primary brain tumors that originate neural or glial stem cells. Classified by the WHO into grades 1 4, these exhibit varying prognoses, with oligodendrogliomas and having better intermediate outcomes, respectively, while glioblastomas associated a poor prognosis. Despite advancements in molecular genetic research have improved diagnosis development of targeted therapies, treating high-grade gliomas...
CaBo is a mannose/glucose-specific lectin purified from seeds of Canavalia bonariensis. In the present work, we report crystal structure determined to atomic resolution in presence X-man, specific ligand. Similar structural characteristics other legume lectins, presented jellyroll motif, metal binding site occupied by calcium and manganese ions close carbohydrate-recognition domain (CRD). vitro test cytotoxicity against glioma cells demonstrated its ability decrease cellular viability...
A lectin from Canavalia virosa, Diocleinae subtribe, was purified by affinity chromatography with Sephadex G-50 matrix and named ConV. The primary structure of ConV obtained mass spectrometry crystals were the vapor diffusion method at 293K belonged to orthorhombic space group P21221 two molecules in its asymmetric unit. presented Rfactor Rfree 18.91% 24.92% respectively, no residues nonallowed regions Ramachandran plot. crystal solved 2.53Å demonstrated be very similar other lectins same...
The three-dimensional structure of Dioclea reflexa seed lectin (DrfL) was studied in detail by a combination X-ray crystallography, molecular docking and dynamics. DrfL purified affinity chromatography using Sephadex G-50 matrix. Its primary obtained mass spectrometry, crystals belonging to orthorhombic space group P212121 were grown the vapor diffusion method at 293K. crystal solved 1.765Å very similar that other lectins from same subtribe. presented Rfactor Rfree 21.69% 24.89%,...
Lectins are a class of carbohydrate-binding proteins or glycoproteins with diverse specificities and functions. The determination characterization the three-dimensional structures these keys to understanding their biological effects. Recent studies have explored anticancer potential Diocleinae lectins (from Leguminoseae family), evaluating antiproliferative effect ability induce glioma cell death via apoptosis autophagy. In this work, structure Dioclea lasiophylla seed lectin (DlyL)...
Lectins are proteins that have as one of their main characteristics recognizing and reversibly binding to carbohydrates. In this work, it was possible purify characterize a lectin from Parkia panurensis (Leguminosae family; Mimosoideae subfamily) seeds by combination the techniques: protein precipitation, along with affinity then ion exchange chromatography using Sepharose-mannose diethylaminoethyl matrices, respectively. The pure lectin, called PpaL, has D-mannose, D-glucose derivatives....
Studies have revealed the dependence of glioma cells on iron, making them sensitive to ferroptosis. Ferroptosis can be triggered by inhibition xc- system, resulting in redox imbalance and membrane lipid peroxidation. The system is composed two coupled proteins, xCT CD98hc. control transporters, such as xCT, CD98hc glycoprotein suggests that molecules targeting glycans may an impact treatment glioma. This study evaluated effect Canavalia brasiliensis (ConBr) lectin C6 compared it with...
Lectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on Sephadex® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and inhibited monosaccharides (D-mannose α-methyl-D-mannoside) glycoproteins (ovalbumin fetuin). Similar to other Diocleinae lectins, has three chains, α, β γ, with mass 25,569 ± 2, 12,998 1 12,588 Da, respectively, no disulfide bonds. The hemagglutinating activity optimal pH 8.0, stable at temperature 70 °C...
The relation structure-activity of the Mimosoideae lectins Parkia platycephala (PPL) and biglobosa (PBL) was analyzed in this study. PBL solved by X-ray crystallography at a resolution 2.1 ÿ, crystal structure belonged to C2221 space group. Structural organization binding sites were also characterized. Specifically, monomer consists three β-prism domains tandemly arranged with each one presenting different carbohydrate recognition domain (CRD). PPL showed antinociceptive activity mouse model...
Vatairea guianensis lectin (VGL), Dalbergiae tribe, is a N-acetyl-galactosamine (GalNAc)/Galactose (Gal) previously purified and characterized. In this work, we report its structural features, obtained from bioinformatics tools, inflammatory effect, rat paw edema model. The VGL model was by homology with the of macrocarpa (VML) as template, used it to demonstrate common characteristics legume lectins, such jellyroll motif presence metal-binding site in vicinity carbohydrate-recognition...
Lectins are a class of proteins or glycoproteins capable recognizing and interacting with carbohydrates in specific reversible manner. Owing to this property, these can interact glycoconjugates present on the cell surface, making it possible decipher glycocode, as well elicit biological effects, such inflammation vasorelaxation. Here, we report structural study mannose/glucose‐specific lectin from Dioclea lasiophylla seeds, DlyL. The aimed evaluate detail interaction DlyL Xman high-mannose...
Lectins are multidomain proteins that specifically recognize various carbohydrates. The structural characterization of these molecules is crucial in understanding their function and activity systems organisms. Most cancer cells exhibit changes glycosylation patterns, lectins may be able to changes. In this work, Dioclea lasiocarpa seed lectin (DLL) was structurally characterized. presented a high degree similarity with other isolated from legumes, presenting jelly roll motif metal-binding...