A5044060740- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Autophagy in Disease and Therapy
- Carbohydrate Chemistry and Synthesis
- Toxin Mechanisms and Immunotoxins
- Maternal and Neonatal Healthcare
- Natural product bioactivities and synthesis
- Environmental Sustainability and Education
- Microbial Metabolism and Applications
- Ginseng Biological Effects and Applications
- Global Maternal and Child Health
- Ubiquitin and proteasome pathways
- Trypanosoma species research and implications
- Male Reproductive Health Studies
- Neuroscience and Neuropharmacology Research
- Health, Nursing, Elderly Care
- RNA Interference and Gene Delivery
- Transcranial Magnetic Stimulation Studies
- Mosquito-borne diseases and control
- Reproductive Health and Technologies
- Transgenic Plants and Applications
- Infant Development and Preterm Care
- Nerve injury and regeneration
- Alkaloids: synthesis and pharmacology
Universidade Federal de Santa Catarina
2012-2024
CaBo is a mannose/glucose-specific lectin purified from seeds of Canavalia bonariensis. In the present work, we report crystal structure determined to atomic resolution in presence X-man, specific ligand. Similar structural characteristics other legume lectins, presented jellyroll motif, metal binding site occupied by calcium and manganese ions close carbohydrate-recognition domain (CRD). vitro test cytotoxicity against glioma cells demonstrated its ability decrease cellular viability...
A lectin from Canavalia virosa, Diocleinae subtribe, was purified by affinity chromatography with Sephadex G-50 matrix and named ConV. The primary structure of ConV obtained mass spectrometry crystals were the vapor diffusion method at 293K belonged to orthorhombic space group P21221 two molecules in its asymmetric unit. presented Rfactor Rfree 18.91% 24.92% respectively, no residues nonallowed regions Ramachandran plot. crystal solved 2.53Å demonstrated be very similar other lectins same...
Lectins are a class of carbohydrate-binding proteins or glycoproteins with diverse specificities and functions. The determination characterization the three-dimensional structures these keys to understanding their biological effects. Recent studies have explored anticancer potential Diocleinae lectins (from Leguminoseae family), evaluating antiproliferative effect ability induce glioma cell death via apoptosis autophagy. In this work, structure Dioclea lasiophylla seed lectin (DlyL)...
Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect antitumor property. The results from this work showed the of lectin extracted Dioclea violacea plant (DVL) on C6 rat glioma cell line. DVL treatment was able induce caspase-3 activation, apoptotic death cellular membrane damage. Furthermore, decreased mitochondrial potential increased number acidic vesicles cleavage LC3, indicating...
The antitumor activity of DVL, a lectin purified from Dioclea violacea seeds, on the U87 human glioma cell line was evaluated and compared with Canavalia ensiformis (ConA). Treatment DVL (10–100 μg/mL; 24–96 h) induced alterations in morphology, decreased numbers clonogenic survival time- concentration-dependent manner. caused significant decreases viability impaired migration. Mechanistically, treatment (12 disrupted mitochondrial electrochemical gradient, without ROS accumulation or...
Lectins are multidomain proteins that specifically recognize various carbohydrates. The structural characterization of these molecules is crucial in understanding their function and activity systems organisms. Most cancer cells exhibit changes glycosylation patterns, lectins may be able to changes. In this work, Dioclea lasiocarpa seed lectin (DLL) was structurally characterized. presented a high degree similarity with other isolated from legumes, presenting jelly roll motif metal-binding...
A glioblastoma (GBM) is a highly malignant primary brain tumor with poor prognosis because of its invasiveness and high resistance to current therapies. In GBMs, abnormal glycosylation patterns are associated malignancy, which allows for the use lectins as tools recognition therapy. More specifically, can interact glycan structures found on cell surface. this context, present work aimed investigate antiglioma potential ConGF, lectin purified from Canavalia grandiflora seeds, against C6...
Lectins from Diocleinae subtribe species (family Leguminosae) are of special interest since they present a wide spectrum biological activities, despite their high structural similarity. During synthesis in plant cells, these proteins undergo post-translational processing resulting the formation three chains (α, β, γ), which constitute lectins' subunits. Furthermore, such wild-type presented as isolectins or with different combinations chains, undermine biotechnological potential. Thus, study...