A5044841765- Growth Hormone and Insulin-like Growth Factors
- Pituitary Gland Disorders and Treatments
- Cancer, Hypoxia, and Metabolism
- Estrogen and related hormone effects
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Microtubule and mitosis dynamics
- Trypanosoma species research and implications
- Fibroblast Growth Factor Research
- Pancreatic function and diabetes
- Immune cells in cancer
- Hypothalamic control of reproductive hormones
- Angiogenesis and VEGF in Cancer
- Insect and Pesticide Research
- Protein Tyrosine Phosphatases
- Autophagy in Disease and Therapy
- Immune Response and Inflammation
- Healthcare and Venom Research
- TGF-β signaling in diseases
- Alkaloids: synthesis and pharmacology
- Bone and Dental Protein Studies
- Telomeres, Telomerase, and Senescence
- Chronic Kidney Disease and Diabetes
- Kruppel-like factors research
- Genetic Syndromes and Imprinting
Universidad Nacional de Córdoba
2015-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2013-2025
Instituto de Investigaciones en Ciencias de la Salud
2013-2025
University of Puerto Rico-Mayaguez
2008-2015
Hospital Quirón Torrevieja
2012
Universidad Nacional de Asunción
2011
Universidad Columbia del Paraguay
2011
In somatotroph tumors, over 50% of patients do not respond satisfactorily to the octreotide (OCT) treatment. Stimulation SSTR2 with OCT triggers anti-proliferative signaling pathways mediated by phosphatase SHP2. This can exercise its functions through STAT3, SHP2/STAT3 subcellular localization being crucial for understanding mechanisms action. We investigated expression SHP2 in somatotrophs role on cell proliferation, effects STAT3 phosphorylation, and localization, using vitro a...
Considering that estradiol is a major modulator of prolactin (PRL) secretion, the aim present study was to analyze role membrane receptor-α (mERα) in regulatory effect this hormone on PRL secretion induced by thyrotropin-releasing (TRH) focusing phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway activation. Anterior pituitary cell cultures from female rats were treated with 17β-estradiol (E(2), 10 nM) and its membrane-impermeable conjugated (E(2)-BSA, alone or coincubated...
Plant lectins have been studied owing to their structural properties and biological effects that include agglutinating activity, antidepressant-like effect antitumor property. The results from this work showed the of lectin extracted Dioclea violacea plant (DVL) on C6 rat glioma cell line. DVL treatment was able induce caspase-3 activation, apoptotic death cellular membrane damage. Furthermore, decreased mitochondrial potential increased number acidic vesicles cleavage LC3, indicating...
Abstract Centrin is a calcium binding protein (CaBP) belonging to the EF‐hand superfamily. As with other proteins within this family, centrin sensor multiple biological target proteins. We chose study Chlamydomonas reinhardtii ( Cr cen) and its interaction melittin (MLT) as model for CaBP complexes due amphipathic properties. Our goal was determine molecular interactions that lead centrin–MLT complex formation, their relative stability, conformational changes associated interaction, when...
Abstract BACKGROUND Prostate smooth muscle cells (SMCs) are strongly involved in the development and progression of benign prostatic hyperplasia prostate cancer. However, their participation prostatitis has not been completely elucidated. Thus, we aimed to characterize response normal SMC bacterial lipopolysaccharide (LPS). METHODS Primary SMCs from rats were stimulated with LPS (0.1, 1, or 10 µg/ml) for 24 48 hr. The phenotype was evaluated by electron microscopy, immunofluorescence,...
Although pituitary adenomas represent 25% of intracranial tumors, they are usually benign, with the mechanisms by which these tumors avoid an invasive profile and metastatic growth development still remaining unclear. In this context, cellular senescence might constitute a plausible explanation for benign nature adenomas. study, we investigated emergence as control mechanism during progression estrogen-induced tumors. The quantification Ki67-immunopositive cells in pituitaries estrogenized...
Pituitary tumor cells have a poor response to the growth inhibitory effect of TGFβ1, possibly resulting from cross talk TGFβ/Smads signal with other signaling pathways, an undescribed mechanism in these tumoral cells. To address this hypothesis, we investigated whether mitogen-activated extracellular signal-regulated kinase (MEK)/ERK1/2 and phosphoinositide-3 kinase/protein B (PI3K/Akt) pathways were able regulate antimitogenic TGFβ1 on GH3B6 treatment decreased cell proliferation induced...
Background: In this report, we explored the role of PKCα and PKCε as mediators phorbol 12-myristate13-acetate (PMA)-induced proliferation in pituitary tumor GH3B6 cells, determined if ERK1/2 Akt pathways were activated. Methods: The cell was estimated by BrdU incorporation cycle progression flow cytometric analysis. We expression membrane cytosolic fractions western blotting. subcellular redistribution both PKC isozymes analyzed confocal microscopy. Results: Incubation with PMA for 15 min...
In this study, we focused on ERβ regulation in the adenohypophysis under different estrogenic milieu, by analyzing whether ER modulates phosphatase and tensin homolog deleted chromosome 10 (PTEN) expression its subcellular localization anterior pituitary glands from Wistar rats GH3 lactosomatotroph cells that over‐expressed ERβ. was regulated a cyclic manner, underwent dynamic changes throughout estrous cycle, with decreased ERβ+ estrus E2 treatment, but increased ovariectomized rats....
The molecular mechanisms underlying the capability of pituitary tumours to avoid unregulated cell proliferation are still not well understood. However, NF-κB transcription factor, which is able modulate only cellular senescence but also tumour progression, has emerged as a targeted candidate. This work was focused on role in during progression experimental tumours. Also, contribution signalling pathways senescence-associated activation and secretory phenotype (SASP) pro-survival-NF-κB target...
Trifluoroacetic acid (TFA) may be the cause of bottleneck in high resolution structure determination for protein-peptide complexes. Fragment based drug design often involves use synthetic peptides which contain TFA (excipient). Our goal was to explore effects this excipient on a model complex: centrin-melittin-TFA. We performed Fourier transform infrared, two-dimensional infrared correlation spectroscopies and spectral simulations analyze amide I'/I'* band components ternary complex....
The molecular mechanisms underlying the ERα nuclear/cytoplasmic pool that modulates pituitary cell proliferation have been widely described, but it is still not clear how targeted to plasma membrane. aim of this study was analyse palmitoylation and membrane (mERα) pool, their participation in E2-triggered membrane-initiated signalling normal tumour growth. Cell cultures were prepared from anterior pituitaries female Wistar rats GH3 cells, treated with 10 nM oestradiol (E2). basal expression...
Abstract Objectives: 17β‐oestradiol interacts with growth factors to modulate lactotroph cell population. However, contribution of isoforms the oestrogen receptor in these activities is not fully understood. In present study, we have established participation α and β receptors effects on proliferation induced by insulin shown involvement NO/sGC/cGMP pathway. Materials methods: Cell cultures were prepared from anterior pituitaries female rats evaluate using bromodeoxyuridine (BrdUrd)...
In the present work, we investigated effect of 17β-estradiol (E₂) and basic fibroblast growth factor 2 (FGF2) on lactotroph cell-proliferative response related membrane-initiated signaling pathway. Anterior pituitary mixed-cell cultures random, cycling 3-mo-old female rats were treated with 10 nM E₂, E₂ membrane-impermeable conjugated BSA (E₂-BSA), PPT (ERα agonist), DPN (ERβ agonist) alone or combined FGF2 (10 ng/ml) for 30 min 4 h. Although our results showed that uptake BrdU into nucleus...
In pituitary adenomas, early recurrences and resistance to conventional pharmacotherapies are common, but the mechanisms involved still not understood. The high expression of epidermal growth factor receptor 2 (HER2)/extracellular signal-regulated kinase (ERK1/2) signal observed in human together with low levels antimitogenic transforming beta (TBR2), encouraged us evaluate effect specific HER2 inhibition trastuzumab on experimental tumor cell its antiproliferative response TGFB1....
Extensive evidence has revealed variations in the number of hormone-producing cells pituitary gland, which occur under physiological conditions such as gestation and lactancy. It been proposed that new differentiate from stem cells. However, exactly how when this takes place is not clear. In work, we used immunoelectron microscopy to identify adult stem/progenitor (SC/P) localized marginal zone (MZ), additionally, detected GFRa2-, Sox2-, Sox9-positive adenoparenchyma (AP) by fluorescence...