The study investigated the antimicrobial activity of essential oil extract Ocimum gratissimum L. (EOOG) against multiresistant microorganisms in planktonic and biofilm form. Hydrodistillation was used to obtain EOOG, analysis chemical composition done by gas chromatography coupled with mass spectrometry (GC/MS) flame ionization detection (GC/FID). EOOG biological verified isolates Staphylococcus aureus Escherichia coli, using four strains for each species. antibacterial action determined...

Croton nepetaefolius is a native plant from northeastern Brazil that belongs to the Euphorbiaceae family. The biological action of this has been extensively explored, being secondary metabolites responsible for its properties alkaloids, diterpenes, and triterpenes. This study aimed evaluate ability casbane diterpene (CD), isolated ethanolic extract C. nepetaefolius, inhibit microbial growth biofilm formation several clinical relevant species (bacteria yeasts). It was found CD possessed...

Most tumors in Carney complex (CNC) are benign, including primary pigmented nodular adrenocortical disease (PPNAD), the main endocrine tumor CNC. Adrenocortical cancer (AC) has never been observed syndrome. Herein, we describe a large Azorean family with CNC caused by point mutation PRKAR1A gene coding for type 1-α (RIα) regulatory subunit of cAMP-dependent protein kinase A, which index patient presented AC.We studied genotype-phenotype correlation CNC.We reported on case series and vitro...

This study aimed to evaluate the abilities of plant and algae lectins inhibit planktonic growth biofilm formation in bacteria yeasts. Initially, ten were tested on Staphylococcus epidermidis, aureus, Klebsiella oxytoca, Pseudomonas aeruginosa, Candida albicans , C. tropicalis at concentrations 31.25 250 μ g/mL. The from Cratylia floribunda (CFL), Vatairea macrocarpa (VML), Bauhinia bauhinioides (BBL), Bryothamnion seaforthii (BSL), Hypnea musciformis (HML) showed activities against least one...

ABSTRACT Objectives To assess the effect of non‐ablative Er:YAG laser therapy on symptoms and microbiological conditions women with recurrent vulvovaginal candidiasis (RVVC). Materials Methods One hundred sixty‐seven (mean age 34.1 ± 8.57 years) participated in this cohort study. Women diagnosed RVVC received four vaginal therapies within 4 months. At baseline, 3 months, 9 months after last assessment, improvement five bothersome related to infection (burning, itching, dyspareunia, dysuria,...

O artigo aborda a importância da extensão universitária como elo essencial entre academia e sociedade, destacando sua evolução para além conexão teoria-prática. Enfatiza o papel ativo na superação de desigualdades, promovendo diálogo ações sociais. Destaca tríade ensino, pesquisa referência um ensino superior holístico. Além disso, discute participação dos estudantes, valorização do conhecimento popular busca por soluções inovadoras universitária. segundo trecho apresenta estudo caso sobre...

Background: The rise of multidrug-resistant (MDR) bacteria poses a significant challenge to global public health, contributing increased morbidity and mortality rates. In this context, the repurposing existing drugs has emerged as promising strategy. study, hydralazine (HDZ), vasodilator used an antihypertensive since 1952, demonstrated antibacterial antibiofilm activity against both Gram-positive Gram-negative bacteria. Methods: (HDZ) was evaluated strains through minimum inhibitory...

Alveolar soft-part sarcoma (ASPS) is a rare, clinically, morphologically, ultrastructurally, and cytogenetically distinctive malignant mesenchymal tumor that most classically occurs in the extremities of adolescents young adults, but has also been described number unusual sites. The molecular signature ASPS specific der(17)t(X;17)(p11.2;q25) translocation, which results fusion TFE3 transcription factor gene (from Xp11) with ASPL at 17q25. ASPL-TFE3 protein encoded by transcript can be...

Lectins are a structurally heterogeneous group of proteins that have specific binding sites for carbohydrates and glycoconjugates. Because their biotechnological potential, lectins widely used in biomedical research. The present study aimed to evaluate the healing potential lectin isolated from marine red alga Bryothamnion seaforthii (BSL). was purified using ion exchange chromatography with DEAE cellulose characterized tandem mass spectrometry. For tests, skin wounds were induced dorsal...

Twelve families that were multiply affected with diffuse idiopathic skeletal hyperostosis (DISH) and/or chondrocalcinosis, identified on the island of Terceira, The Azores, potentially supporting hypothesis 2 disorders share common etiopathogenic factors. present study was undertaken to investigate this hypothesis.One hundred three individuals from 12 unrelated assessed. Probands patients attending Rheumatic Diseases Clinic, Hospital de Santo Espírito, in Azores. Family members assessed by...

The initial colonization of the tooth by streptococci involves their attachment to adsorbed components acquired pellicle. Avoiding this adhesion may be successful in preventing caries at early stages. Salivary mucins are glycoproteins that when absorbed onto hydroxyapatite provide binding sites for certain bacteria. Algal lectins especially interesting oral antiadhesion trials because great stability and high specificity mucins. This work aimed evaluate potential two algal inhibit adherence...

Aim: Initial colonization of the tooth surface by streptococci involves attachment these bacteria to adsorbed salivary components acquired pellicle. In dental biofilm this adhesion may also involve lectin‐like components, present on organisms, which bind complementary carbohydrates tooth. Therefore, work aimed evaluate potential six lectins, extracted from seeds Leguminosae family members, inhibit adherence five species pellicle in vitro. Methods and Results: The lectins used were Canavalia...

Lectins are a structurally heterogeneous group of highly specific carbohydrate-binding proteins. Due to their great biotechnological potential, lectins widely used in biomedical research. The purpose the present study was evaluate healing potential lectin Bauhinia variegata (nBVL) and its recombinant isoform (rBVL-1). Following surgical creation dorsal skin wounds, seven groups mice were submitted topical treatment for 12 days with lectin, D-galactose, BSA saline. animals anesthetized...

Surface colonization is an essential step in biofilm development. The ability of oral pathogens to adhere tooth surfaces directly linked with the presence specific molecules at bacterial surface that can interact enamel acquired pellicle ligands. In light this, aim this study was verify inhibitory and antibiofilm action lectins from Diocleinaesubtribe against Streptococcus mutans oralis. planctonic cells assessed using Canavaliaensi formis (ConA), Canavalia brasiliensis (ConBr), maritima...

The aim of the present work was to study in vitro effect native and recombinant Bauhinia variegata var. lectins inhibiting early adhesion Streptococcus mutans, sanguis sobrinus experimentally acquired pellicle.Native lectin from B. (BVL) purified by affinity chromatography extract seeds. (rBVL-I) expressed E. coli strain BL21 (DE3) a genomic clone encoding mature gene using vector pAE-bvlI. Recombinant protein deposited inclusion bodies solubilized subsequently chromatography. rBVL-I...

This study evaluated the antimicrobial effect of 3 β ,6 ,16 -trihydroxylup-20(29)-ene (CLF1), a triterpene isolated from Combretum leprosum Mart., in inhibiting planktonic growth and biofilms Gram positive bacteria Streptococcus mutans S. mitis . The activity was assessed by determining minimum inhibitory concentration (MIC) bactericidal (MBC). antibiofilm potential determined quantifying total biomass enumerating biofilm-entrapped viable bacteria. In addition, acute toxicity CLF1 on Artemia...

The carbohydrate-binding activity of the algal lectins from closely related red marine species Bryothamnion triquetrum (BTL) and seaforthii (BSL) was used to differentiate human colon carcinoma cell variants with respect their membrane glyco-receptors. These interacted cells tested in a dose-dependent manner. Moreover, fluorescence spectra both clearly differentiated as shown by FACS profiles. Furthermore, observed confocal microscopy, BTL BSL bound surface glycoproteins underwent intense...

Biofilms are assemblages of microorganisms and their associated extracellular products at an interface typically with abiotic or biotic surface. The study the morphology biofilms is important because they processes biofouling, corrosion, catalysis, pollutant transformation, dental caries, drug resistance, so forth. In literature, have been examined by atomic force microscopy (AFM), which has proven to be a potent tool different aspects biofilm development on solid surfaces. this work, we...

CDH3/P-cadherin is a classical cadherin. Overexpression of which has been associated with proliferative lesions high histological grade, decreased cell polarity and poor survival patients breast cancer. In vitro studies showed that it can be up-regulated by ICI 182,780, suggesting the lack ERα signalling responsible for aberrant P-cadherin overexpression its role in inducing cancer invasion migration. However, mechanism ER-signalling inhibition leads to expression still unknown. The aim this...

To study the interactions between a Rhizobium tropici strain and lectins isolated from seeds of Canavalia ensiformis (ConA) brasiliensis (ConBr), lectin fluorescence assay was performed. In addition, an experiment designed to evaluate effect two on bacterial growth. Both were found bind R. cells, but inhibited by D-mannose. Interestingly, only ConBr stimulated growth in proportion concentrations used (15.6-500 μg/mL), stimulation D-mannose as well. Structure/Function analyses bioinformatics...

Bacteria form biofilms as an adaptive mechanism in response to environmental changes. Streptococcus mutans is the biofilm-forming bacterium that primarily associated with dental caries. The expression of important genes by bacteria different from planktonic cells. Lectins are proteins bind specifically carbohydrates and may have biological activities on bacterial cells, acting antibacterial anti-biofilm agents. ConM (Canavalia maritima lectin) a protein able inhibit growth biofilm formation...