A5090582013- Agricultural pest management studies
- Insect-Plant Interactions and Control
- Insect Pest Control Strategies
- Insect Resistance and Genetics
- Advanced Proteomics Techniques and Applications
- Agricultural Practices and Plant Genetics
- Protein Structure and Dynamics
- Influenza Virus Research Studies
- RNA Research and Splicing
- Antimicrobial Peptides and Activities
- Computational Drug Discovery Methods
- Virus-based gene therapy research
- Venomous Animal Envenomation and Studies
- Bacteriophages and microbial interactions
- Ion channel regulation and function
- Fossil Insects in Amber
- Virology and Viral Diseases
- Mass Spectrometry Techniques and Applications
- interferon and immune responses
- Research on scale insects
- RNA and protein synthesis mechanisms
- Plant and animal studies
- Insect behavior and control techniques
- Hymenoptera taxonomy and phylogeny
- Receptor Mechanisms and Signaling
European Molecular Biology Laboratory
2018-2023
European Molecular Biology Organization
2022-2023
Queen Mary University of London
2022
Tamil Nadu Agricultural University
2013-2021
GlaxoSmithKline (Germany)
2019-2020
Institute of Entomology
2017-2020
National University of Singapore
2013-2017
Visiting Nurse Service of New York
2012
Abstract Adenosine triphosphate (ATP) plays fundamental roles in cellular biochemistry and was recently discovered to function as a biological hydrotrope. Here, we use mass spectrometry interrogate ATP-mediated regulation of protein thermal stability solubility on proteome-wide scale. Thermal proteome profiling reveals high affinity interactions ATP substrate an allosteric modulator that has widespread influence complexes their stability. Further, develop strategy for profiling, discover...
Abstract Neurodegeneràve diseases such as amyotrophic lateral sclerosis (ALS) are oten associated with mutàons in proteins that stress granules. Stress granules condensates formed by liquid-liquid phase separàon which, when aberrant, can lead to altered condensàon behaviours and disease phenotypes. Here, we identified lipoamide, a small molecule which specifically prevents cytoplasmic of granule proteins. Thermal proteome profiling showed lipoamide preferentially stabilises intrinsically...
Abstract Reversible protein phosphorylation is an important mechanism for regulating (dis)assembly of biomolecular condensates. However, condensate-specific phosphosites remain largely unknown, thereby limiting our understanding the underlying mechanisms. Here, we combine solubility proteome profiling with phosphoproteomics to quantitatively map several hundred enriched in either soluble or condensate-bound subpopulations, including a subset modulating protein–RNA interactions. We show that...
Negative-stranded RNA viruses can establish long-term persistent infection in the form of large intracellular inclusions human host and cause chronic diseases. Here, we uncover how cellular stress disrupts metastable host-virus equilibrium induces viral replication a culture model mumps virus. Using combination cell biology, whole-cell proteomics, cryo-electron tomography, show that factories are dynamic condensates identify largely disordered phosphoprotein as driver their assembly. Upon...
In biological systems, liquid and solid-like biomolecular condensates may contain the same molecules but their behaviour, including movement, elasticity, viscosity, is different on account of distinct physicochemical properties. As such, it known that phase transitions affect function material properties can be tuned by several factors temperature, concentration, valency. It is, however, unclear if some are more efficient than others at regulating behaviour. Viral infections good systems to...
Detecting ligand-protein interactions in living cells is a fundamental challenge molecular biology and drug research. Proteome-wide profiling of thermal stability as function ligand concentration promises to tackle this challenge. However, current data analysis strategies use preset thresholds that can lead suboptimal sensitivity/specificity tradeoffs limited comparability across datasets. Here, we present method based on statistical hypothesis testing curves, which provides control the...
Protein aggregates have negative implications in disease. While reductionist experiments increased our understanding of aggregation processes, the systemic view biological context is still limited. To extend this understanding, we used mass spectrometry-based proteomics to characterize and disaggregation human cells after non-lethal heat shock. Aggregation-prone proteins were enriched nuclear proteins, high proportion intrinsically disordered regions, molecular mass, isoelectric point,...
Natriuretic peptides (NPs) are potent vasoactive hormones, which maintain pressure-volume homoeostasis. Snake venom NPs exhibit distinct biological activity compared with mammalian due to subtle changes in their sequences. We recently identified a new NP from krait (KNP), an unusual 38-residue long C-terminal tail, has propensity form α-helix. KNP mediates vasodilation via receptor (NPR) independent mechanisms on pre-contracted aortic strips contrast classical NPs. The infusion of...
Heart failure (HF) is associated with high morbidity and mortality. Dysfunction of blood pressure and/or volume homeostatic processes result in lower perfusion congestion. Treatment strategies exerting differential effects on mechanisms are critical handling patients HF. Atrial natriuretic peptides (ANPs) a key hormone maintaining circulation. It binds to NP receptor-A (NPR-A) vasculature, kidneys nervous system lowers volume. exerts concentration-dependent pharmacological activity, only...
Abstract Natriuretic peptides (NP) play important roles in human cardiac physiology through their guanylyl cyclase receptors NPR‐A and NPR‐B. Described herein is a bifunctional O‐glycosylated natriuretic peptide, TcNPa, from Tropidechis carinatus venom it unusually targets both Characterization using specific glycosidases ETD‐MS identified the glycan as galactosyl‐β(1‐3)‐ N ‐acetylgalactosamine (Gal‐GalNAc) was α‐linked to C‐terminal threonine residue. TcNPa contains characteristic NP...
Highly publicized recommendations favor the use of diuretics as a first-line or add-on agent in management hypertension, particularly among black patients and with resistant hypertension. Failure to follow such guidelines might contribute high rates uncontrolled This study assessed diuretic prescribing patterns sample hypertension who were identified from population home care recipients. The was conducted an urban health organization. Participants black, aged 21 80 years, had diagnosis...
The predatory potential of aphid lion, Chrysoperla zastrowi sillemi (Neuroptera: Chrysopidae) against Aphis punicae in pomegranate was studied and the observation showed that all larval stages predator were found to feed on A. punicae. Furthermore, third instar grub C. with developmental period 3.4 days had consumed a maximum 106.3 aphids followed by second first grubs which 68.1 30.7 during their development 3.2 2.7 days, respectively. Further, single consume total 205.1 its 9.3 days....
Summary Many viruses can establish long-term persistent infections in the human host and cause chronic diseases. Here we combine cell biology, whole-cell proteomics cryo-electron tomography to uncover how cellular stress disrupts host-virus equilibrium infection induces viral replication, using a model of negative-stranded RNA viruses, mumps virus. We show that phosphorylation largely disordered phosphoprotein coincides with increased partitioning polymerase into pre-formed liquid-like...
Natriuretic peptides (NPs) play a vital role in the regulation of pressure-volume homeostasis. They mediate vasodilation and increase water-electrolyte excretion through kidneys by activating membrane bound guanylyl cyclase receptors (NPR). Apart from three mammalian isoforms, snake venom NPs with distinct pharmacological functions have been characterized. This study describes molecular mechanism physiological action novel NP krait (Bungarus flaviceps) called KNP. peptide has conserved...
There is little work on the bioefficacy of mineral oil management whitefly in vegetable crops. The oil, a safer material than synthetic chemical pesticides, was determined against Bemisia tabaci (Gennadius) okra [Abelmoschus esculentus (L.) Moench]. Laboratory and pot culture studies were conducted to test alone or combination with other materials okra. A 2% concentration + neem Pongamia glabra seed produced 95% 93.33% mortality at 48 h after treatment under laboratory conditions. In...
Field observation at weekly intervals on the occurrence of mango hopper population under Ultra high density planting system was carried out R & D farm, Jain Irrigation Systems Limited, Udumalpet, Tamil Nadu during 2014. The incidence noted from 4th week January which reaches its peak (80 hoppers/20 panicles) fourth February corresponding with 8th standard week. declined second March disappeared May. Reoccurrence observed in first September 4.8 hoppers /20 panicles vegetative phase after...
Different colour sticky traps are commonly used for monitoring the population of many sucking pests such as thrips, whiteflies, leaf miner adults and aphids. They have also been to monitor natural enemies associated with pests. Presently, incidence in pomegranate using is wanting. Hence, study was conducted planting at orchard Tamil Nadu Agricultural University, Coimbatore during 2016, different laden like yellow, pale green, orange red. The were placed five locations every week, location...
Nucleotide triphosphates (NTPs) regulate numerous biochemical processes in cells as (co-)substrates, allosteric modulators, biosynthetic precursors, and signaling molecules. Apart from its roles energy source fueling cellular biochemistry, adenosine triphosphate (ATP), the most abundant NTP cells, has been reported to affect macromolecular assemblies, such protein complexes membrane-less organelles. Moreover, both ATP guanosine (GTP) have recently shown dissolve aggregates. However,...