A5008886759- Pancreatic function and diabetes
- Genetic and Kidney Cyst Diseases
- Bacteriophages and microbial interactions
- Renal cell carcinoma treatment
- Advanced Biosensing Techniques and Applications
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Photodynamic Therapy Research Studies
- Kruppel-like factors research
- Renal and related cancers
CS Diagnostics
2022-2024
Helmholtz Zentrum München
2019-2021
Technical University of Munich
2019
Abstract Diabetes mellitus affects one in eleven adults worldwide. Most suffer from Type 2 which features elevated blood glucose levels and an inability to adequately secrete or respond insulin. Insulin producing β-cells have primary cilia are implicated the regulation of metabolism, insulin signaling secretion. To better understand how β-cell affect handling, we ablate mature by deleting key component Ift88 . Here report that homeostasis secretion deteriorate over 12 weeks post-induction....
Islet vascularization is essential for intact islet function and glucose homeostasis. We have previously shown that primary cilia directly regulate insulin secretion. However, it remains unclear whether they are also implicated in vascularization. At eight weeks, murine Bbs4 -/- islets show significantly lower intra-islet capillary density with enlarged diameters. Transplanted exhibit delayed re-vascularization reduced vascular fenestration after engraftment, partially impairing permeability...
Abstract Tertiary lymphoid structures (TLS) have recently emerged as a predictive biomarker in cancer immunotherapy. Their consideration preclinical drug development is impeded by rare and transient appearance random distribution, making them difficult to analyze with conventional sampling methods. We applied whole-organ tissue clearing 3D staining of B T cell clusters comprehensively identify TLS KP GEMM NSCLC using Light Sheet Fluorescence Microscopy (3D LSFM). To further investigate...
Rationale Accumulating evidence point to a role for primary cilia in endothelial cell function. Islet vascularization is an important determinant of islet function and glucose homeostasis. We have previously shown that β-cell directly regulate insulin secretion. However, it unclear whether are also implicated thus contribute Objective To characterize the vascularization. Methods Results At four weeks, Bbs4 −/− islets show markedly lower intra-islet capillary density with enlarged diameters....
Abstract Summary: We present a novel light sheet fluorescence microscopy (LSFM)-based imaging platform and corresponding set of procedures for greatly improved preclinical investigation the biodistribution drug candidates by example an EpCAM-targeting antibody. Methods: An anti-EpCAM antibody (G.8.8R) was applied intravenously to mice. Whole mice or individual organs were optically cleared following protocols, which we termed Rapid Optical Clearing Kit Enhanced Tissue Scanning (ROCKETS). The...