A5049586965- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Ion Channels and Receptors
- COVID-19 Clinical Research Studies
- Mitochondrial Function and Pathology
- SARS-CoV-2 and COVID-19 Research
- Nerve injury and regeneration
- Neuropeptides and Animal Physiology
- Lipid Membrane Structure and Behavior
- Adipose Tissue and Metabolism
- Neurotransmitter Receptor Influence on Behavior
- Cholinesterase and Neurodegenerative Diseases
- Neuroscience and Neural Engineering
- Reproductive Biology and Fertility
- Amyotrophic Lateral Sclerosis Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Calcium signaling and nucleotide metabolism
- Cellular transport and secretion
- Pharmacological Receptor Mechanisms and Effects
- Heart Rate Variability and Autonomic Control
- Sirtuins and Resveratrol in Medicine
- Pancreatic function and diabetes
- Treatment of Major Depression
- Computational Drug Discovery Methods
University of Castilla-La Mancha
2017-2024
Universidad Autónoma de Madrid
2012-2022
Hospital Universitario de La Princesa
2011-2018
GTx (United States)
2017
Universidade de Santiago de Compostela
2004-2009
SARS-CoV-2 virus requires host proteases to cleave its spike protein bind ACE2 target through a two-step furin-mediated entry mechanism. Aprotinin is broad-spectrum protease inhibitor that has been employed as antiviral drug for other human respiratory viruses. Also, it important anti-inflammatory properties inhibiting the innate immunity contact system.This was multicentre, double-blind, randomized trial performed in four Spanish hospitals comparing standard treatment versus + aprotinin...
Studies have reported the importance of mitochondria in sperm functionality. However, for some species, glycolytic pathway appears to be as important oxidative phosphorylation ATP synthesis and kinetics. These mechanisms not been fully elucidated bovine spermatozoa. Therefore, aim this study was evaluate role synthesis, movement patterns, homeostasis epididymal spermatozoa specimens. We observed that mitochondrial uncoupling with protonophores significantly reduced levels. these levels were...
The serotonin (5-hydroxytryptamine; 5-HT) 2A receptor is a cell surface class A G protein-coupled that regulates multitude of physiological functions the body and target for antipsychotic drugs. Here we found by means fluorescence resonance energy transfer immunoprecipitation studies 5-HT<sub>2A</sub>-receptor homodimerized in live cells, which linked with its antagonist-dependent fingerprint both binding signaling. Some antagonists, like atypical antipsychotics clozapine risperidone,...
During brain ischemia, oxygen and glucose deprivation induces calcium overload, extensive oxidative stress, neuroinflammation, and, finally, massive neuronal loss. In the search of a neuroprotective compound to mitigate this loss, we have designed synthesized new multitarget hybrid (ITH14001) directed at reduction overload by acting on two regulators homeostasis; mitochondrial Na+/Ca2+ exchanger (mNCX) L-type voltage dependent channels (VDCCs). This is CGP37157 (mNCX inhibitor) nimodipine...
A library of 4,6-diaryl-1,4-dihydropyridines was synthesized using a CAN-catalyzed, Hantzsch-related three component reaction starting from ammonium acetate, β-dicarbonyl compounds and variety α,β-unsaturated ketones including chalcones, their vinylogs heteroanalogues. These lack the structural features needed for vascular activity were found to prevent calcium overload behave as neuroprotective agents. One compounds, bearing 2-thienyl substituent at C-4, showed highest also moderate...
In recent years, the “non-autonomous motor neuron death” hypothesis has become more consolidated behind amyotrophic lateral sclerosis (ALS). It postulates that cells other than neurons participate in pathology. fact, involvement of autonomic nervous system is fundamental since patients die sudden death when they unable to compensate for cardiorespiratory arrest. Mitochondria are thought play a role physiopathology ALS, as compromised multiple ALS models different cell types, and it also...
Chondroitin sulfate (CS) proteoglycans (CSPGs) are the most abundant PGs of brain extracellular matrix (ECM). Free CS could be released during ECM degradation and exert physiological functions; thus, we aimed to investigate effects on voltage- current-clamped rat embryo hippocampal neurons in primary cultures. We found that elicited a whole-cell Na(+)-dependent inward current (ICS) produced drastic cell depolarization, cytosolic calcium transient ([Ca(2+)]c). Those were similar those by...
Altered synaptic transmission with excess glutamate release has been implicated in the loss of motoneurons occurring amyotrophic lateral sclerosis (ALS). Hyperexcitability or hypoexcitability from mice carrying ALS mutation SOD1(G93A) (mSOD1) also reported. Here we have investigated excitability, ion currents, and kinetics exocytotic fusion pore chromaffin cells postnatal day 90 to 130 mSOD1 mice, when motor deficits are already established. With respect wild-type (WT), had a decrease...
Chronic ethanol (EtOH) consumption has been associated with deleterious effects on the cardiovascular system by abnormal calcium (Ca2+) handling. Store-operated Ca2+ entry (SOCE) is related to remodeling which leads hypertension development, and coupling between STIM-1 (ER sensor) Orai-1 (channel pore) a key mechanism control SOCE through of store-operated channels (SOCCs). However, role STIM-1/Orai-1-mediated its cross-talk EtOH-triggered vascular remain poorly understood. We address this...
Adrenal chromaffin cells (CCs) have been used extensively in studies aimed at revealing the intricacies of Ca2+ -dependent early and late steps regulated exocytosis. They also served as invaluable models to study kinetics single-vesicle exocytotic events infer characteristics opening closing fusion pore. We here tested hypothesis that stimulation room temperature CCs from mice C57BL/6 with physiological acetylcholine (ACh) other secretagogues (dimethylphenylpiperazinium, high K+ , muscarine,...
SARS-CoV-19 infection provokes a variety of symptoms; most patients present mild/moderate symptoms, whereas small proportion progress to severe illness with multiorgan failure accompanied by metabolic disturbances requiring ICU-level care. Given the importance disease, researchers focused on identifying severity-associated biomarkers in infected as well markers associated suffering long-COVID. However, little is known about presence that remain few years after SARS-CoV-2 once fully recover...
From experiments performed at room temperature, we know that the buffering of Ca2+ by mitochondria contributes to shaping bulk cytosolic calcium transient ([Ca2+]c) and secretion transients chromaffin cells stimulated with depolarizing pulses. We also mitochondrial transporters release catecholamine are faster 37°C respect temperature. Therefore, planned this investigation gain further insight into contribution [Ca2+]c transients, using some novel experimental conditions have not been yet...
At early life, the adrenal chromaffin cells respond with a catecholamine surge under hypoxic conditions. This response depends on Ca(2+) entry through voltage-activated calcium channels (VACCs). We have investigated here three unresolved questions that concern this in rat embryo (ECCs): 1) relative contribution of L (α1D, Cav1.3), N (α1B, Cav2.2), and PQ (α1A, Cav2.1) to whole cell current (ICa); 2) N/PQ cytosolic elevations triggered by hypoxia (Δ[Ca(2+)]c); 3) role non-L high-VACCs...
Upon repeated application of short ACh pulses to C57BL6J mouse chromaffin cells, the amperometrically monitored secretory responses promptly decayed a steady-state level around 25% initial response. A subsequent K+ pulse, however, overcame such decay. These data suggest that cells have ready release-vesicle pool is selectively recruited by physiological neurotransmitter ACh. The ACh-sensitive vesicle refilled and maintained rate Ca2+ delivery from mitochondria cytosol, through mitochondrial...