A5076676591- Tissue Engineering and Regenerative Medicine
- Clinical Nutrition and Gastroenterology
- Cancer Cells and Metastasis
- Digestive system and related health
- 3D Printing in Biomedical Research
- Infant Nutrition and Health
- Electrospun Nanofibers in Biomedical Applications
- Cancer Research and Treatments
- Vascular Malformations and Hemangiomas
- Helicobacter pylori-related gastroenterology studies
- Pediatric Hepatobiliary Diseases and Treatments
- Esophageal and GI Pathology
- Viral gastroenteritis research and epidemiology
- Tracheal and airway disorders
- Congenital gastrointestinal and neural anomalies
- Vascular Malformations Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Tumors and Oncological Cases
- Disaster Response and Management
- Congenital Diaphragmatic Hernia Studies
- Enhanced Recovery After Surgery
- Neonatal Respiratory Health Research
- Barrier Structure and Function Studies
- Child Nutrition and Feeding Issues
- Cancer, Hypoxia, and Metabolism
The University of Texas Health Science Center at Houston
2013-2025
Memorial Hermann
2018-2024
Texas Medical Center
2024
Short and Associates (United States)
2022
Baylor College of Medicine
2019-2020
Children's National
2015-2019
University of Southern California
2008-2016
LAC+USC Medical Center
2010-2016
Children's Hospital of Los Angeles
2010-2015
State Street (United States)
2010
Short bowel syndrome (SBS) is a devastating condition in which insufficient small intestinal surface area results malnutrition and dependence on intravenous parenteral nutrition. There an increasing incidence of SBS, particularly premature babies newborns with congenital anomalies. Tissue-engineered intestine (TESI) offers therapeutic alternative to the current standard treatment, transplantation, has potential solve its biggest challenges, namely donor shortage life-long immunosuppression....
Human intestinal enteroids (HIEs) are gaining recognition as physiologically relevant models of the epithelium. While HIEs from adults used extensively in biomedical research, few studies have infants. Considering dramatic developmental changes that occur during infancy, it is important to establish represent infant characteristics and physiological responses. We established jejunal surgical samples performed comparisons using RNA sequencing (RNA-Seq) morphologic analyses. then validated...
Tissue-engineered small intestine (TESI) has successfully been used to rescue Lewis rats after massive bowel resection. In this study, we transitioned the technique a mouse model, allowing investigation of processes involved during TESI formation through transgenic tools available in species. This is necessary step toward applying human therapy. Multicellular organoid units were derived from intestines mice and transplanted within abdomen on biodegradable polymers. Immunofluorescence...
Background Regeneration of lost periodontium is the ultimate goal periodontal therapy. Advances in tissue engineering have demonstrated multilineage potential and plasticity adult stem cells located apparatus. However, it remains unclear how epigenetic mechanisms controlling signals determine specification cell lineage decisions. To date, no data are available on micro‐ RNA (mi ) activity behind human‐derived dental (DSCs). Material Methods In this study, we isolated ligament cells, pulp...
ABSTRACT Human noroviruses (HuNoVs) are a significant cause of epidemic and sporadic acute gastroenteritis worldwide. The lack reproducible culture system hindered the study HuNoV replication pathogenesis for almost half-century. This barrier was overcome with our successful cultivation multiple strains in human intestinal enteroids (HIEs), which has significantly advanced research. We optimized media conditions generated genetically modified HIE cultures to enhance HIEs. Building upon these...
The complexity of intestinal cytoarchitecture and function poses significant challenges for the creation bioengineered small intestine. Techniques generating human organoids (HIOs) resembling intestine have been previously reported. HIOs contain epithelium mesenchyme but lack other critical components functional such as enteric nervous system (ENS), immune cells, vasculature, microbiome. Two independent research groups published distinct methods to innervate with an ENS. Here we discuss a...
Tissue-engineered esophagus (TEE) may serve as a therapeutic replacement for absent foregut. Most prior studies have favored microdesigned biomaterials and yielded epithelial growth alone. None generated human TEE with mesenchymal components. We hypothesized that sufficient progenitor cells might only require basic support successful generation of murine TEE.Esophageal organoid units (EOUs) were isolated from or esophagi implanted on polyglycolic acid/poly-l-lactic acid collagen-coated...
Human intestinal enteroids (HIEs) are gaining recognition as physiologically relevant models of the epithelium. While HIEs from adults used extensively in biomedical research, few studies have infants. Considering dramatic developmental changes that occur during infancy, it is important to establish represent infant characteristics and physiological responses. We established jejunal surgical samples performed comparisons using RNA sequencing (RNA-Seq) morphologic analyses. validated...
Tissue-engineered small intestine (TESI) has successfully been used to rescue Lewis rats after massive bowel resection, resulting in return preoperative weights within 40 days.1 In humans, resection can result short syndrome, a functional malabsorptive state that confers significant morbidity, mortality, and healthcare costs including parenteral nutrition dependence, liver failure cirrhosis, the need for multivisceral organ transplantation.2 this paper, we describe document our protocol...
Human noroviruses (HuNoVs) are a significant cause of epidemic and sporadic acute gastroenteritis worldwide. The lack reproducible culture system hindered the study HuNoV replication pathogenesis for almost half-century. This barrier was overcome with our successful cultivation multiple strains in human intestinal enteroids (HIEs), which has significantly advanced research. We optimized media conditions generated genetically-modified HIE cultures to enhance HIEs. Building upon these...
Aim: Loss of colon reservoir function after colectomy can adversely affect patient outcomes. In previous work, human fetal intestinal cells developed epithelium without mesenchyme following implantation in mice. However, for humans, postnatal tissue would be the preferred donor source. We generated tissue-engineered (TEC) from organoid units. Materials & methods: Organoid units were prepared waste specimens, loaded onto biodegradable scaffolds and implanted into immunocompromised After 4...
Traumatic coagulopathy (TC) occurs in 24% to 38% of adults and is associated with up a six-fold increase mortality. This study's purpose was determine the incidence pediatric TC its impact on mortality.A retrospective review (2004-2009) all trauma patients from our Level I center performed. Coagulopathy defined as an international normalized ratio 1.5 or higher activated partial thromboplastin time more than 36 seconds platelets less 100,000/mm. Clinical outcomes were compared between...
Short bowel syndrome (SBS) is a morbid and mortal condition characterized in most patients by insufficient intestinal surface area. Current management strategies are inadequate, but tissue-engineered small intestine (TESI) offers potential therapy. A barrier to translation of TESI the generation scalable mucosal area significantly increase nutritional absorption. Fibroblast growth factor 10 (Fgf10) critical essential for development gastrointestinal tract. We hypothesized that overexpression...
To determine the effect of VEGF overexpression on tissue-engineered small intestine (TESI) formation.Organoid units were isolated from intestines 2-week-old transgenic mouse pups capable inducible, ubiquitous (CMV-Cre/rtTA/tet(0)-VEGF) and implanted into nonobese diabetic/severe combined immunodeficiency mice. Resulting TESI explanted at 2 4 weeks, studied by histology, tissue ELISA immunofluorescence.At weeks postimplantation, mucosa group formed rudimentary villi more crypts compared with...
Summary The obesity epidemic continues to affect millions of children and adolescents. Non‐surgical options do not result in significant or sustained weight loss; thus bariatric surgery has become increasingly utilized. Limited data exist regarding safety for paediatric surgery, especially outside National Institutes Health ( NIH )‐funded centres. We hypothesized that the perioperative outcomes patients undergoing laparoscopic sleeve gastrectomy LSG ) at our free‐standing children's hospital...