A5025632281- Viral gastroenteritis research and epidemiology
- Infant Nutrition and Health
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Cancer Cells and Metastasis
- Digestive system and related health
- Cancer, Hypoxia, and Metabolism
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- EEG and Brain-Computer Interfaces
- Cancer Research and Treatments
- Genetic factors in colorectal cancer
- Congenital Anomalies and Fetal Surgery
- Cardiovascular Issues in Pregnancy
- Bioactive Compounds and Antitumor Agents
- Escherichia coli research studies
- Problem and Project Based Learning
- Genomics and Phylogenetic Studies
- T-cell and Retrovirus Studies
- Viral-associated cancers and disorders
- Microbial Natural Products and Biosynthesis
- Animal Virus Infections Studies
- Autophagy in Disease and Therapy
- Tea Polyphenols and Effects
- Pancreatic and Hepatic Oncology Research
The Wallace H. Coulter Department of Biomedical Engineering
2013-2025
Georgia Institute of Technology
2013-2025
Baylor College of Medicine
2018-2025
Emory University
2024
Texas A&M University
2019
Dr. D.Y. Patil Vidyapeeth, Pune
2015
Human intestinal enteroids (HIEs) are gaining recognition as physiologically relevant models of the epithelium. While HIEs from adults used extensively in biomedical research, few studies have infants. Considering dramatic developmental changes that occur during infancy, it is important to establish represent infant characteristics and physiological responses. We established jejunal surgical samples performed comparisons using RNA sequencing (RNA-Seq) morphologic analyses. then validated...
Spinal cord injury (SCI) can lead to permanent motor and sensory deficits. Following the initial traumatic insult, secondary mechanisms characterized by persistent heightened inflammation are initiated continued pervasive cell death tissue damage. Anti-inflammatory drugs such as methylprednisolone (MP) used clinically have ambiguous benefits with debilitating side effects. Typically, these administered systemically at high doses, resulting in toxicity paradoxically increased inflammation....
Abstract Rotaviruses (RVs) cause life-threatening diarrhea in infants and children worldwide. Recent biochemical epidemiological studies underscore the importance of histo-blood group antigens (HBGA) as both cell attachment susceptibility factors for globally dominant P[4], P[6], P[8] genotypes human RVs. How these interact with HBGA is not known. Here, our crystal structures P[4] a neonate-specific P[6] VP8*s alone complex H-type I reveal unique glycan binding site that conserved allows ABH...
Human noroviruses (HuNoVs) are the leading cause of acute gastroenteritis. In immunocompetent hosts, symptoms usually resolve within 3 days; however, in immunocompromised persons, HuNoV infection can become persistent, debilitating, and sometimes life-threatening. There no licensed therapeutics for due to a near half-century delay its cultivation. Treatment chronic immunosuppressed patients anecdotally includes nitazoxanide, broad-spectrum antimicrobial treatment parasite-induced Despite...
In vitro models, such as primary cells and continuous cell lines routinely used for evaluating drug candidates, have limitations in their translational relevance to human diseases. Organotypic cultures are increasingly being assess therapeutics various cancers infectious Monitoring cytotoxicity is crucial development, several commercially available kits assessment offer distinct advantages limitations. Given the complexity of organoid cultures, including donor-driven variability, we...
ABSTRACT Human noroviruses (HuNoVs) are a significant cause of epidemic and sporadic acute gastroenteritis worldwide. The lack reproducible culture system hindered the study HuNoV replication pathogenesis for almost half-century. This barrier was overcome with our successful cultivation multiple strains in human intestinal enteroids (HIEs), which has significantly advanced research. We optimized media conditions generated genetically modified HIE cultures to enhance HIEs. Building upon these...
ABSTRACT Human noroviruses (HuNoVs) are the leading cause of acute gastroenteritis worldwide. Currently, there no targeted antivirals for treatment HuNoV infection. Histo-blood group antigens (HBGAs) on intestinal epithelium cellular attachment factors HuNoVs; molecules that block binding HuNoVs to HBGAs thus have potential be developed as antivirals. milk oligosaccharides (HMOs) glycans in human with structures analogous HBGAs. HMOs been shown act decoy receptors prevent multiple enteric...
ABSTRACT Human norovirus (HuNoV) is a leading cause of gastroenteritis worldwide and associated with significant morbidity, mortality, economic impact. There are currently no licensed antiviral drugs for the treatment HuNoV-associated gastroenteritis. The HuNoV protease plays critical role in initiation virus replication by cleaving polyprotein. Thus, it an ideal target developing small-molecule inhibitors. While rupintrivir, potent inhibitor several picornavirus proteases, effectively...
Human intestinal epithelial organoids (enteroids and colonoids) are tissue cultures used for understanding the physiology of human epithelium. Here, we explored effect on transcriptome common variations in culture methods, including extracellular matrix substrate, format, segment, differentiation status, patient heterogeneity. RNA-sequencing datasets from 276 experiments performed 37 enteroid colonoid lines 29 patients were aggregated several groups Texas Medical Center. DESeq2 gene set...
Prostate cancer is a major cause of cancer-related mortality in men. Even though current therapeutic management has contributed to reducing mortality, additional intervention strategies are warranted further improve the outcomes. To this end, we have investigated efficacy dicaffeoylquinic acids, ingredients Yerba Mate ( Ilex paraguariensis ), an evergreen cultivated South America, leaves which used prepare tea/coffee-like drink. Of various analogs tested, 4,5-dicaffeoylquinic acid...
Treatment of aggressive glioblastoma brain tumors is challenging, largely due to diffusion barriers preventing efficient drug dosing tumors. To overcome these barriers, bacterial carriers that are actively motile and programmed migrate localize tumor zones were designed. These can induce apoptosis via hypoxia-controlled expression a suppressor protein p53 pro-apoptotic drug, Azurin. In xenograft model human in rats, carrier therapy conferred significant survival benefit with 19% overall...
Worldwide prevalence of esophageal adenocarcinomas with high rates mortality coupled increased mutations in cells warrants investigation to understand deregulation cell signaling pathways leading cancer. To this end, the current study was undertaken unravel death signatures using model human adenocarcinoma line-OE33. The strategy involved targeting key epigenetic modulator SIRT1, a histone deacetylase by small molecule inhibitor - sirtinol. Sirtinol induced dose-dependent inhibition...
Human intestinal enteroids (HIEs) are gaining recognition as physiologically relevant models of the epithelium. While HIEs from adults used extensively in biomedical research, few studies have infants. Considering dramatic developmental changes that occur during infancy, it is important to establish represent infant characteristics and physiological responses. We established jejunal surgical samples performed comparisons using RNA sequencing (RNA-Seq) morphologic analyses. validated...
Human noroviruses (HuNoVs) are a significant cause of epidemic and sporadic acute gastroenteritis worldwide. The lack reproducible culture system hindered the study HuNoV replication pathogenesis for almost half-century. This barrier was overcome with our successful cultivation multiple strains in human intestinal enteroids (HIEs), which has significantly advanced research. We optimized media conditions generated genetically-modified HIE cultures to enhance HIEs. Building upon these...
Background: Colorectal cancer is the third largest cause of cancer-related mortality worldwide. Although current treatments with chemotherapeutics have allowed for management colorectal cancer, additional novel are essential. Intervening metabolic reprogramming observed in cancers called “Warburg Effect,” one strategies considered to combat cancers. In pathway, pyruvate dehydrogenase kinase (PDK1) plays a pivotal role. Identification and characterization PDK1 inhibitor paramount importance....
Human noroviruses (HuNoVs) are the leading cause of acute gastroenteritis worldwide. Currently, there no targeted antivirals for treatment HuNoV infection. Histo-blood group antigens (HBGAs) on intestinal epithelium cellular attachment factors HuNoVs; molecules that block binding HuNoVs to HBGAs thus have potential be developed as antivirals. milk oligosaccharides (HMOs) glycans in human with structures analogous HBGAs. HMOs been shown act decoy receptors prevent multiple enteric pathogens...
Among the most common mucosal viral infections in infants are rotavirus, one of main causes severe gastroenteritis and children up to 5 years, respiratory syncytial virus (RSV), leading lower tract infections. Both human milk bovine derived factors may provide protection against More recently, a similar activity proteins was suggested for SARS-CoV-2. The goal current study test antiviral milkfat globule membrane (MFGM) RSV SARS-CoV-2 further characterize MFGM-enriched whey identify which...
ABSTRACT Human norovirus (HuNoV) infection is a global health and economic burden. Currently, there are no licensed HuNoV vaccines or antiviral drugs available. The protease encoded by the genome plays critical role in virus replication cleaving polyprotein is, therefore, an excellent target for developing small molecule inhibitors. While rupintrivir, potent small-molecule inhibitor of several picornavirus proteases, effectively inhibits GI.1 protease, it order magnitude less effective...
Deregulation of signaling pathways due to mutations sets the cell on a path neoplasia. Therefore, recent reports increased observed in esophageal tissue reflects enhanced risk tumor formation. In fact, adenocarcinoma esophagus has been rise lately. Increase mortality paucity efficacious drugs for this cancer prompted us discover molecular signatures combat malady. To end, we chose resveratrol-a polyphenol with anticancer property-and studied its impact three lines (OE33, OE19 and FLO-1) by...
The prevalence of colorectal cancer has increased world-wide with high rates mortality and morbidity. In the absence efficacious drugs to treat this neoplasia, there is an imminent need discover molecules multifaceted effects. To end, we opted study effect steroidal saponins such as Polyphyllins. We performed anticancer activity studies three analogs Polyphyllins: Polyphyllin D (PD), II (PII) G (PG). Here show potent PD, PII (IC50 0.5-1 µM) PG 3 in inhibiting viability adenocarcinoma cells...
Pancreatic cancer is one of the most aggressive forms and seventh leading cause deaths worldwide. ductal adenocarcinoma (PDAC) accounts for over 90% pancreatic cancers. Most cancers are recalcitrant to radiation, chemotherapy, immunotherapy, highlighting urgent need novel treatment options this deadly disease. To end, we screened a library kinase inhibitors in PDAC cell lines PANC-1 BxPC-3 identified two highly potent molecules: Aurora inhibitor AT 9283 (AT) EGFR WZ 3146 (WZ). Both exhibited...