A5072608448- HIV/AIDS drug development and treatment
- Hepatitis B Virus Studies
- HIV Research and Treatment
- Hepatitis C virus research
- Biochemical and Molecular Research
- Nanoplatforms for cancer theranostics
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- Nerve injury and regeneration
- Cancer Research and Treatments
- Nanoparticle-Based Drug Delivery
- Tissue Engineering and Regenerative Medicine
- Protein Interaction Studies and Fluorescence Analysis
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Cytomegalovirus and herpesvirus research
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Advanced Drug Delivery Systems
- Neuroscience and Neural Engineering
- Glioma Diagnosis and Treatment
- Tea Polyphenols and Effects
- Quinazolinone synthesis and applications
The Wallace H. Coulter Department of Biomedical Engineering
2014-2025
Georgia Institute of Technology
2015-2025
Duke University
2018
Indiana University – Purdue University Indianapolis
2018
Purdue University West Lafayette
2018
Emory and Henry College
2016
Mercer University
2009
Mercer University Health Sciences Center
2008-2009
Kidwai Memorial Institute of Oncology
2006
Veterans Health Administration
2003-2005
A novel anti-hepatitis B virus (anti-HBV) agent, 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil (L-FMAU), was synthesized and found to be a potent anti-HBV anti-Epstein-Barr agent. Its in vitro potency evaluated 2.2.15 H1 cells for activities, respectively. In cytotoxicity MT2, CEM, 2.2.15, also assessed, the results indicated high antiviral selectivities of L-FMAU these cells.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and Biological Evaluation of 2',3'-Dideoxy-L-pyrimidine Nucleosides as Potential Antiviral Agents against Human Immunodeficiency Virus (HIV) Hepatitis B (HBV)Tai-Shun Lin, Mei-Zhen Luo, Mao-Chin Liu, S. Balakrishna Pai, Ginger E. Dutschman, Yung-Chi ChengCite this: J. Med. Chem. 1994, 37, 6, 798–803Publication Date (Print):March 1, 1994Publication History Published online1 May 2002Published inissue 1 March...
2'-Fluoro-5-methyl-beta-L-arabinofuranosyl uracil (L-FMAU) was discovered to have potent antiviral activity against hepatitis B virus (HBV). L-FMAU more than its D-enantiomer and produced dose-dependent inhibition of the viral DNA replication in 2.2.15 cells (human HepG2 with HBV genome), a 50% inhibitory concentration 0.1 microM. There no effect on transcription or protein synthesis. In cell system, did not show any toxicity up 200 microM, whereas toxic, 50 Repeated treatments at 1 microM...
Since 2'-fluoro-5-methyl-β-l-arabinofuranosyluracil (l-FMAU) has been shown to be a potent anti-HBV agent in vitro, it was of interest study the structure−activity relationships related nucleosides. Thus, series 1-(2-deoxy-2-fluoro-β-l-arabinofuranosyl)pyrimidine nucleosides have synthesized and evaluated for antiviral activity against HBV 2.2.15 cells. For this study, l-ribose initially used as starting material. Due commercial cost l-ribose, we developed an efficient procedure preparation...
Spinal cord injury (SCI) can lead to permanent motor and sensory deficits. Following the initial traumatic insult, secondary mechanisms characterized by persistent heightened inflammation are initiated continued pervasive cell death tissue damage. Anti-inflammatory drugs such as methylprednisolone (MP) used clinically have ambiguous benefits with debilitating side effects. Typically, these administered systemically at high doses, resulting in toxicity paradoxically increased inflammation....
See Moon and Bradbury (doi:10.1093/brain/awy067) for a scientific commentary on this article. Many hundreds of thousands people around the world are living with long-term consequences spinal cord injury they need effective new therapies. Laboratory research in experimental animals has identified large number potentially translatable interventions but transition to clinic is not straightforward. Further evidence efficacy more clinically-relevant lesions required gain sufficient confidence...
Prostate cancer is a major cause of cancer-related mortality in men. Even though current therapeutic management has contributed to reducing mortality, additional intervention strategies are warranted further improve the outcomes. To this end, we have investigated efficacy dicaffeoylquinic acids, ingredients Yerba Mate ( Ilex paraguariensis ), an evergreen cultivated South America, leaves which used prepare tea/coffee-like drink. Of various analogs tested, 4,5-dicaffeoylquinic acid...
Worldwide prevalence of esophageal adenocarcinomas with high rates mortality coupled increased mutations in cells warrants investigation to understand deregulation cell signaling pathways leading cancer. To this end, the current study was undertaken unravel death signatures using model human adenocarcinoma line-OE33. The strategy involved targeting key epigenetic modulator SIRT1, a histone deacetylase by small molecule inhibitor - sirtinol. Sirtinol induced dose-dependent inhibition...
Summary. The emergence of drug‐resistant virus in hepatitis B (HBV) patients treated with lamivudine is well documented. In this study, we determined the mutations occurring tyrosine–methionine–aspartate–aspartate (YMDD) amino acid motif HBV DNA polymerase gene, as upstream and downstream region, breakthrough during therapy. Thirty‐one Turkish (20 HBeAg positive, 11 negative anti‐HBe positive) chronic infection who completed at least 104 weeks treatment were investigated. All received...
β-l-2′,3′-Dideoxyadenosine, β-l-2′,3′-didehydro-2′,3′-dideoxyadenosine and related compounds were synthesized in a stereoselective manner. These tested vitro against HBV 2.2.15 cell line HIV-1 PBM CEM cells. It was found that β-l-2′,3′-dideohydro-2′,3′-dideoxyadenosine (7) exhibited significant anti-HIV (EC50 0.38 μM cells) anti-HBV activity 1.2 μM).
The proximity ligation assay (PLA) has previously been used for the sensitive and specific detection of single proteins. In order to adapt PLA methods cell surfaces, we have generated multivalent peptide-oligonucleotide-phycoerythrin conjugates ('burrs') that can bind adjacent one another on a surface be ligated together form unique amplicons. Real-time PCR burr events specifically identified as few 100 Bacillus anthracis, 10 subtilis 1 cereus spore. Burrs should prove generally useful...
Background: Colorectal cancer is the third largest cause of cancer-related mortality worldwide. Although current treatments with chemotherapeutics have allowed for management colorectal cancer, additional novel are essential. Intervening metabolic reprogramming observed in cancers called “Warburg Effect,” one strategies considered to combat cancers. In pathway, pyruvate dehydrogenase kinase (PDK1) plays a pivotal role. Identification and characterization PDK1 inhibitor paramount importance....
In this study we investigated nanoliposome as an approach to tailoring the pharmacology of cerivastatin a disease-modifying drug for pulmonary arterial hypertension (PAH). Cerivastatin encapsulated liposomes with average diameter 98 ± 27 nm were generated by thin film and freeze-thaw process. The nanoliposomes demonstrated sustained drug-release kinetics in vitro inhibited proliferation artery (PA) smooth muscle cells significantly less cellular cytotoxicity compared free cerivastatin. When...
Catalase in albumin microspheres were formulated for intravenous administration to antagonize the effects of over-production reactive oxygenated species (ROS) such as hydrogen peroxide (H(2)O(2)) septic shock. The aim was increase effective half-life catalase and take advantage phagocytic uptake encapsulated by vascular endothelium. prepared spray-drying. evaluated particle size, shape surface morphology scanning electron microscopy (SEM), drug encapsulation efficiency, chemical stability,...