A5052324644- Lipid Membrane Structure and Behavior
- Advanced Chemical Sensor Technologies
- Spectroscopy and Quantum Chemical Studies
- Ionic liquids properties and applications
- Metabolomics and Mass Spectrometry Studies
- Nanopore and Nanochannel Transport Studies
- Metabolism and Genetic Disorders
- Analytical Chemistry and Chromatography
- thermodynamics and calorimetric analyses
- Essential Oils and Antimicrobial Activity
- Sphingolipid Metabolism and Signaling
- Microbial Metabolic Engineering and Bioproduction
European Spallation Source
2015-2020
Institut Laue-Langevin
2015-2020
Lund University
2018-2020
We have characterized and compared the structures of ergosterol- cholesterol-containing 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes before after interaction with amphiphilic antifungal drug amphotericin B (AmB) using neutron reflection. AmB inserts into both pure POPC sterol-containing in lipid chain region does not significantly perturb structure membranes. By selective per-deuteration lipids/sterols, we show that extracts ergosterol but cholesterol from bilayers to a...
1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), an unsaturated acyl chain containing lipid, is often the predominant lipid in eukaryotic cell membranes which it crucial for fluidity of under physiological conditions. Commercially available, partially deuterated [D31 ]1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine ([D31 ]POPC) does not provide sufficient isotopic contrast detailed structural studies multicomponent through neutron techniques. Herein, a relatively straightforward and...
Sterols regulate several physico-chemical properties of biological membranes that are considered to be linked function. Ergosterol is the main sterol molecule found in cell yeasts and other fungi. Like cholesterol mammalian cells, ergosterol has been proposed have an ordering condensing effect on saturated phospholipid membranes. The effects investigated extensively result increase membrane thickness lipid acyl chain order. Less information available Neutron Diffraction (ND) was used...