A5084530038- Lipid Membrane Structure and Behavior
- Nanoparticle-Based Drug Delivery
- Protein Structure and Dynamics
- Characterization and Applications of Magnetic Nanoparticles
- RNA Interference and Gene Delivery
- Supramolecular Self-Assembly in Materials
- Sphingolipid Metabolism and Signaling
- Spectroscopy and Quantum Chemical Studies
- Advanced biosensing and bioanalysis techniques
- Iron oxide chemistry and applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Ferrocene Chemistry and Applications
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Magnetic and transport properties of perovskites and related materials
- Protein Interaction Studies and Fluorescence Analysis
- Electronic and Structural Properties of Oxides
- Cellular transport and secretion
- Metabolomics and Mass Spectrometry Studies
- Conservation Techniques and Studies
- Advanced NMR Techniques and Applications
- Metal complexes synthesis and properties
- ZnO doping and properties
- Polymer Surface Interaction Studies
- Electrostatics and Colloid Interactions
University of Perugia
2023-2024
European Spallation Source
2022-2023
University of Copenhagen
2018-2022
Institut Laue-Langevin
2016-2021
Paul Scherrer Institute
2021
University of Naples Federico II
2014-2017
Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase
2015-2017
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2015
University of Florence
2015
Despite the growing technological interest of polydopamine (dopamine melanin)-based coatings for a broad variety applications, factors governing particle size, shape, and electronic properties this bioinspired multifunctional material have remained little understood. Herein, we report detailed characterization growth, morphology, paramagnetic as function dopamine concentration nature buffer (pH 8.5). Dynamic Light Scattering data revealed an increase in hydrodynamic radii (Rh) melanin...
Cationic nanovectors loaded with Ru-based nucleolipids exert a high growth-inhibitory activity against human cancer cells (MCF-7 (A), WiDr (B), and HeLa (C)).
Looking for new metal-based anticancer treatments, in recent years many ruthenium complexes have been proposed as effective and safe potential drugs. In this context we recently developed a novel approach the vivo delivery of Ru(III) complexes, preparing stable ruthenium-based nucleolipidic nanoaggregates endowed with significant antiproliferative activity. Herein describe cellular response to our ruthenium-containing formulations selected models human breast cancer. By vitro bioscreens...
Cholesterol is an essential component of mammalian membranes and known to induce a series physicochemical changes in the lipid bilayer. Such include formation liquid-ordered phases with increased thickness configurational order as compared liquid-disordered phases. For saturated membranes, cholesterol molecules localize close head group-tail interface. However, presence polyunsaturated lipids was recently shown promote relocation toward inner interface between two bilayer leaflets. Here,...
Abstract SARS-CoV-2 spike proteins are responsible for the membrane fusion event, which allows virus to enter host cell and cause infection. This process starts with binding of extramembrane domain angiotensin-converting enzyme 2 (ACE2), a receptor highly abundant in lungs. In this study, Spike (sSpike) was injected on model membranes formed by supported lipid bilayers presence absence soluble part ACE2 (sACE2), structural features were studied at sub-nanometer level neutron reflection. all...
Hydrophobins are fungal proteins whose functions mainly based on their capability to self-assemble into amphiphilic films at hydrophobic-hydrophilic interfaces (HHI). It is widely accepted that class I hydrophobins form amyloid-like structures, named rodlets, which hundreds of nanometers long, packed ordered lateral assemblies and do not exhibit an overall helical structure. We studied the self-assembly Class hydrophobin Vmh2 from Pleurotus ostreatus in aqueous solutions by dynamic light...
Superparamagnetic Iron Oxide Nanoparticles (SPIONs) are performing contrast agents for Magnetic Resonance Imaging (MRI). A functionalization strategy SPIONs based on hydrophobic interactions is a versatile approach easily extendable to several kinds of inorganic nanoparticles and suitable obtaining stable biocompatible systems. Here we report the original preparation functionalized with an 8 nm radius exploiting interaction between phosphocholine inner amphiphilic. With respect other...
SuperParamagnetic Iron Oxide Nanoparticles (SPIONs) represent a suitable system for several applications especially in nanomedicine.
Hybrid semiconductor-ferromagnetic insulator heterostructures are interesting due to their tunable electronic transport, self-sustained stray field, and local proximitized magnetic exchange. In this work, we present lattice-matched hybrid epitaxy of InAs/EuS analyze the atomic-scale structure characteristics. The Fermi level at interface is found be close InAs conduction band in gap EuS, thus preserving semiconducting properties. Both neutron X-ray reflectivity measurements show that overall...
The bacterial cytoplasmic membrane is the innermost and mainly composed of three different phospholipid species, i.e., phosphoethanolamine (PE), phosphoglycerol (PG), cardiolipin (CL). In particular, PG CL are responsible for negative charge often targets cationic antimicrobial agents. growing resistance bacteria toward available antibiotics requires development new more efficient antibacterial drugs. this context, studying physicochemical properties pivotal understanding drug–membrane...
Structural characterization and synthetic difficulties behind the obtainment of iron oxide-gold nanoparticles for biomedical applications.
Sterols regulate several physico-chemical properties of biological membranes that are considered to be linked function. Ergosterol is the main sterol molecule found in cell yeasts and other fungi. Like cholesterol mammalian cells, ergosterol has been proposed have an ordering condensing effect on saturated phospholipid membranes. The effects investigated extensively result increase membrane thickness lipid acyl chain order. Less information available Neutron Diffraction (ND) was used...
In vitro characterization of membrane proteins requires experimental approaches providing mimics the microenvironment that encounter in native membranes. this context, supported lipid bilayers provide a suitable platform to investigate by broad range surface-sensitive techniques such as neutron reflectometry (NR), quartz crystal microbalance with dissipation monitoring (QCM-D), surface plasmon resonance (SPR), atomic force microscopy (AFM), and fluorescence microscopy. Nevertheless,...
Abstract Dynamic interactions of proteins with lipid membranes are essential regulatory events in biology, but remain rudimentarily understood and particularly overlooked membrane proteins. The ubiquitously expressed protein Na + /H -exchanger 1 (NHE1) regulates intracellular pH (pH i ) dysregulation linked to e.g. cancer cardiovascular diseases. NHE1 has a long, cytosolic domain carrying membrane-proximal region described as lipid-interacting (LID), yet, the LID structure underlying...