A5101660729- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Insect-Plant Interactions and Control
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Insect Pest Control Strategies
- Plant Parasitism and Resistance
- Virus-based gene therapy research
- Plant and animal studies
- Invertebrate Immune Response Mechanisms
- Coleoptera Taxonomy and Distribution
- Studies on Chitinases and Chitosanases
- Insect symbiosis and bacterial influences
- Animal Genetics and Reproduction
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- Hepatitis B Virus Studies
- Insect Utilization and Effects
- RNA Interference and Gene Delivery
- Transgenic Plants and Applications
- Advanced biosensing and bioanalysis techniques
- Corneal Surgery and Treatments
- Marine Sponges and Natural Products
- Dengue and Mosquito Control Research
- Allelopathy and phytotoxic interactions
Qingdao Agricultural University
2015-2025
Wenzhou Medical University
2022
Zhejiang Taizhou Hospital
2022
Qingdao University
2016
Qingdao Municipal Hospital
2016
Shandong First Medical University
2011-2012
Inner Mongolia Agricultural University
2012
Shandong Eye Hospital
2011-2012
Atomic Energy (Canada)
2011
Shandong Academy of Agricultural Sciences
2011
The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), is native to the Americas. It has rapidly invaded 47 African countries and 18 Asian since first detection of invasion into Nigeria Ghana in 2016. regarded as a super pest based on its host range (at least 353 plants), inherent ability survive wide habitats, strong migration ability, high fecundity, rapid development resistance insecticides/viruses gluttonous characteristics. inherently superior biological characteristics FAW...
The application of RNA interference (RNAi) technology for pest control is environmentally friendly and accurate. However, the efficiency RNAi often inconsistent unreliable, finding a suitable carrier element considered critical to success in overcoming biotic abiotic barriers reach target site. fall armyworm, Spodoptera frugiperda (FAW), which one most important global agricultural pests, has recently spread rapidly other parts world. In this study, method improve stability dsRNA complex was...
Bemisia tabaci is an important agricultural sucking pest, and it develops serious resistance to various insecticides. Although cytochrome P450 was involved in the cyantraniliprole, limited studies have been conducted on B. tabaci. In present study, piperonyl butoxide significantly increased toxicity of cyantraniliprole. activities two resistant populations were 1.97- 2.17-fold higher than that susceptible population. Among 79 genes, CYP6CX3 expressions 3.08- 3.67-fold When knocked down,...
Previous studies showed that several members of the S100A family are involved in neovascularization and tumor development. This study checked whether low concentrations S100A8 or S100A9 has any effect on behaviour vascular endothelial cells. A human umbilical cell (HUVEC) line was used to measure bioactivity related angiogenesis, such as proliferation, migration, vessel formation. In concentration range up 10 μ g/mL, either each alone combination, proteins promoted proliferation HUVEC cells...
The beet armyworm Spodoptera exigua is a polyphagous caterpillar that causes serious damage to many species of crops and vegetables. To gain insight into how this insect differs from less harmful oligophagous species, we generated chromosome-level assembly compared it closely related with the same or different feeding habits. Based on Illumina Pacific Biosciences data Hi-C technology, 425.6 Mb genome sequences were anchored oriented 31 linkage groups, an N50 length 14.8 Mb. A total 24,649...
Abstract Targeted deletion of the mef2c gene results in a small left ventricle and complete loss right (Lin et al. [1997] Science 276:1404–1407). Absence is from defective differentiation cells secondary heart field. Our studies dysmorphogenesis uncovered morphological transcriptional abnormalities at transition cardiac crescent to linear‐tube stage heart. Use cgata6LacZ transgene demonstrated that lacZ ‐positive cells, which normally mark precursors atrioventricular canal adjacent regions...
Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cosmopolitan insect pest causing serious damage to crop production. Cytochromes P450 (CYPs) of B. are widely known be involved in the metabolic resistance variety insecticides, continuously increasing difficulty controlling this pest. In study, four genes (CYP6CM1, CYP6CX1, CYP6CX3, and CYP402C1) exhibited correlations with imidacloprid. We have focused on trying understand function metabolism capacity CYP402C1. The expression profiles...
Objective: To investigate the impact of a Croton tiglium extract on cellular proliferation and apoptosis in nonsmall cell lung cancer line (A549) vitro. Methods: A seed methanol was prepare assessed for effects A549 cells regarding proliferation, apoptotic rates, expression related genes proteins using real-time PCR immunofluorescence. Results: The tested inhibited dose- time-dependent manner, with significant elevation indexes at various concentrations after 24 h. In addition, rates both...
Cyantraniliprole is the first diamide insecticide to have cross-spectrum activity against a broad range of insect orders. The insecticide, like other diamides, selectively acts on ryanodine receptor, destroys Ca2+ homeostasis, and ultimately causes death. Although expression regulations genes associated with calcium signaling pathways are known be involved in response little regarding function calmodulin (CaM), typical sensor central regulating stress insects insecticide. In this study, we...
Abstract A suspended cell line from Trichoplusia ni embryos was established, and its susceptibility to Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) infection investigated. This had characteristics distinct the BTI‐Tn5Bl‐4 (Tn5Bl‐4) T. in growth, showed approximately same responses AcMNPV infection, production of occlusion bodies, levels recombinant protein expression. No clumps were observed at maximum density late‐log phase shake‐flask or T‐flask cultures, thus cells...
Ca2+-binding proteins (CaBPs) are widely distributed as Ca2+ sensor relay that regulate various cellular processes, including homeostasis. Diamide insecticides such cyantraniliprole kill insects by disrupting the homeostasis in muscle cells. However, less attention has been paid to roles of CaBPs response insecticides. In this study, two CaBP genes (BtCaBP1 and BtCaBP2) were identified whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), their functions investigated. After...
Helicoverpa armigera is a globally-important crop pest with WZ (female)/ZZ (male) sex chromosome system. The absence of discernible sexual dimorphism in its egg and larval stages makes it impossible to address any sex-related theoretical applied questions before pupation unless W-specific sequence marker available for diagnosis. To this end, we used one pair morphologically pre-sexed pupae PCR-screen 17 non-transposon transcripts selected from 4855 W-linked candidate reads identified by...
Peptidoglycan recognition proteins (PGRPs) are a class of molecules that play critical role in insect immunity. Understanding the function PGRPs is important to improve efficiency microbial insecticides. In this study, we investigated PGRP-LB (a long type PGRP) immunity against viruses using Spodoptera exigua and multiple nucleopolyhedrovirus (SeMNPV) as an insect-virus model. We cloned identified gene from S. exigua; consisted 7 exons encoded polypeptide 234 amino acids with signal peptide...
Serum-free cultures of insect cells play an important role in the fields protein engineering, medicine, and biology. In this paper, suspension cell line QB-Tn9-4s Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae) was successfully adapted to serum-free Sf-900 III medium passaged for 52 generations. The grew faster. Their population doubling time shortened from 27.4 hr serum-containing 24.1 hr, their maximal density increased by 1.83-fold, reaching 3.50 × 106 cells/mL culture T-flasks. readily...