A5076111165- Identification and Quantification in Food
- Biosensors and Analytical Detection
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Alzheimer's disease research and treatments
- Bee Products Chemical Analysis
- Entomopathogenic Microorganisms in Pest Control
- Advanced biosensing and bioanalysis techniques
- Computational Drug Discovery Methods
- Drug Transport and Resistance Mechanisms
- Fungal Biology and Applications
- Fermentation and Sensory Analysis
- Listeria monocytogenes in Food Safety
- Microbial Metabolic Engineering and Bioproduction
- Blood properties and coagulation
- Enzyme Production and Characterization
- Signaling Pathways in Disease
- Biofuel production and bioconversion
- Phytochemicals and Antioxidant Activities
- Salmonella and Campylobacter epidemiology
- Gold and Silver Nanoparticles Synthesis and Applications
- Probiotics and Fermented Foods
- Cholinesterase and Neurodegenerative Diseases
- Bacterial Genetics and Biotechnology
- Wound Healing and Treatments
Hebei Agricultural University
2014-2025
Nanjing Maternity and Child Health Care Hospital
2024
Nanjing Medical University
2024
Guangdong Medical College
2020
Central South University
2019
Xiangya Hospital Central South University
2019
Fudan University
1991-2010
State Key Laboratory of Genetic Engineering
2010
University of Tennessee at Knoxville
2004-2009
Knoxville College
2007
Genetic studies of Tibetans, an ethnic group with a long-lasting presence on the Tibetan Plateau which is known as highest plateau in world, may offer unique opportunity to understand biological adaptations human beings high-altitude environments. We conducted genome-wide study 1,000,000 genetic variants 46 Tibetans (TBN) and 92 Han Chinese (HAN) for identifying signals (HAAs) genomes. discovered most differentiated between TBN HAN at chromosome 1q42.2 2p21. EGLN1 (or HIFPH2, MIM 606425)...
Gamma-secretase cleavage of beta-amyloid precursor protein (APP) is crucial in the pathogenesis Alzheimer disease, because it decisive step formation C terminus (Abeta). To better understand molecular events involved gamma-secretase APP, this study we report identification a new intracellular long Abeta species containing residues 1-46 (Abeta46), which led to novel zeta-cleavage site between known gamma- and epsilon-cleavage sites within transmembrane domain APP. Our data clearly demonstrate...
beta-Amyloid precursor protein apparently undergoes at least three major cleavages, gamma-, epsilon-, and the newly identified zeta-cleavage, within its transmembrane domain to produce secreted beta-amyloid (Abeta). However, roles of epsilon- zeta-cleavages in formation Abeta relationship among these namely zeta-, gamma-cleavages, remain elusive. We investigated issues by attempting determine turnover intermediate products generated presence or absence known gamma-secretase inhibitors. By...
Abstract It has been hypothesized that different C‐terminus of β‐amyloid peptide (Aβ) may be generated by γ‐secretase activities. Recently, we have identified a new ζ‐cleavage site at Aβ46, leading to an important finding the Aβ is produced series sequential cleavages. This prompted us examine effects known inhibitors on steps γ‐secretase‐mediated cleavages and specifically their formation turnover intermediate 46 . Our results demonstrate some inhibitors, such as L‐685,458 III‐31C well IV...
Abstract Based on the single factor experiment, Box-Behnken central composite design of response surface methodology was applied to optimize conditions ethanol fermentation sweet sorghum juice. Taking production as evaluation index, this method investigated main factors, such temperature, pH, and inoculum, influence fermentation. The experimental data indicated that application model equation for evaluating optimum values verified effectively. parameters juice converting were obtained as:...
A novel nucleic acid isothermal amplification method based on saltatory rolling circle (SRCA) for rapid and visual detection of Alicyclobacillus acidoterrestris in apple juice was established. Fourteen A. strains 44 non-A. were used to confirm the specificity. The sensitivity SRCA 4.5 × 101 CFU/mL by observing white precipitate with naked eye, while it 100 fluorescence visualization. limit artificially inoculated 7.1 via visualization fluorescence, respectively. Compared traditional PCR...
To search for novel Vip3A proteins controlling insect pests.A pair of universal primers was designed based on the conserved regions five vip3A genes. Amplified products were digested with HindIII and EcoR enzymes so as to confirm different restriction fragment length polymorphism (RFLP) patterns used identify vip3A-type The gene types 606 Bacillus thuringiensis strains screened three RFLP successfully identified. Two genes found one these, vip3Aa19, further characterized its product...
Abstract This article reviews the current status of fuel ethanol production and discusses fermentation mechanism technology being applied to process worldwide. In addition, this review addresses from lignocelluloses as raw material, such crop residuals, grass, wood waste paper, etc.
Health concerns and financial losses caused by mushroom poisoning have been reported worldwide.
Tripartite motif-containing protein 59 (TRIM59) is a biomarker for multiple tumors with crucial roles. However, the specific role of TRIM59 in germ cells remains largely unknown. Here, we investigated effects and underlying regulatory mechanisms on using mouse spermatogonial cell line GC-1. Our results demonstrated that promoted proliferation inhibited apoptosis GC-1 cells. Mechanistically, maintained behaviors through ubiquitination AXIN1 to activate β-catenin signaling. Furthermore,...
The observations that three major cleavages within the transmembrane domain of APP, namely, gamma-cleavage, -cleavage, and newly identified zeta-cleavage, are involved in generation secreted Abeta40 Abeta42 prompted us to determine how calpain inhibitor III MDL 28170 influences these Abeta formation. With use a cell culture system, our data demonstrate 1) at either high concentrations, or low range early time points, inhibits formation Abeta42. However, this effect is due inhibition...
Overwhelming evidence supports the amyloid hypothesis of Alzheimer's disease that stipulates relative level 42 amino acid beta-amyloid peptide (Abeta(42)) in relationship to Abeta(40) is critical pathogenesis disease. While it clear multi-subunit gamma secretase responsible for cleavage precursor protein (APP) into Abeta(42) and Abeta(40), exact molecular mechanisms regulating production various Abeta species remain elusive. To elucidate underlying mechanisms, we replaced individual residues...
Abstract The main purpose of this study was to investigate the effect raspberry extract on wound healing and compare it with that ellagic acid. elimination excess free radicals key preventing inflammation; cellular antioxidation activity evaluated using an oxidative stress damage cell model. Cell proliferation ability measured WST-1 assay, migration capacity determined scratch assay. A mouse model used verify healing. antioxidant ((50.31±3.17) μg/mL) slightly lower than acid ((44.59±2.38)...
It is urgently necessary to develop convenient, reliable, ultrasensitive and specific methods of ochratoxin A determination in food safety owing its high toxicity. In the present study, an labeled-free fluorescent aptamer sensor combining real-time fluorescence with strand displacement amplification (SDA) was fabricated for OTA. presence OTA, OTA-aptamer combines thus opening hairpins. Then, SDA primers specifically bind hairpin stem, which used subsequent as a template. initiated under...