A5101592614- CRISPR and Genetic Engineering
- Medical Imaging Techniques and Applications
- Plant Virus Research Studies
- Radiomics and Machine Learning in Medical Imaging
- Virus-based gene therapy research
- Advanced MRI Techniques and Applications
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Medical Image Segmentation Techniques
- Chromosomal and Genetic Variations
- Infrastructure Maintenance and Monitoring
- Geological Modeling and Analysis
- Image and Signal Denoising Methods
- Mosquito-borne diseases and control
- Lung Cancer Diagnosis and Treatment
- Ultrasound Imaging and Elastography
- Hematopoietic Stem Cell Transplantation
- Asphalt Pavement Performance Evaluation
- Advanced biosensing and bioanalysis techniques
- Ultrasound and Hyperthermia Applications
- Robotic Path Planning Algorithms
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Multiple Myeloma Research and Treatments
- Atomic and Subatomic Physics Research
The First Affiliated Hospital, Sun Yat-sen University
2021-2025
Sun Yat-sen University
2021-2025
Shenzhen Institutes of Advanced Technology
2023-2024
Zhongshan Hospital
2024
Fudan University
2024
Mean texture depth (MTD) is pivotal in assessing the skid resistance of asphalt pavements and ensuring road safety. This study focuses on developing an automated system for extracting features evaluating MTD based pavement images. The contributions this work are threefold: firstly, it proposes economical method to acquire three-dimensional (3D) data; secondly, enhances 3D image processing techniques formulates that represent various aspects texture; thirdly, establishes multivariate...
Abstract Genome editing technologies hold tremendous potential in biomedical research and drug development. Therefore, it is imperative to discover gene tools with superior cutting efficiency, good fidelity, fewer genomic restrictions. Here, we report a CRISPR/Cas9 from Faecalibaculum rodentium , which characterized by simple PAM (5′-NNTA-3′) guide RNA length of 21–22 bp. We find that FrCas9 could achieve comparable efficiency specificity SpCas9. Interestingly, the presents palindromic...
Abstract Human papillomavirus (HPV) integration is a critical step in cervical cancer development; however, the oncogenic mechanism at genome-wide transcriptional level still poorly understood. In this study, we employed integrative analysis on multi-omics data of six HPV-positive and three HPV-negative cell lines. Through HPV detection, super-enhancer (SE) identification, SE-associated gene expression extrachromosomal DNA (ecDNA) investigation, aimed to explore influence integration. We...
Prime editing (PE) holds tremendous potential in the treatment of genetic diseases because it can install any desired base substitution or local insertion/deletion. However, full-length PE effector size (6.3-kb) is beyond packaging capacity adeno-associated virus (AAV), hindering its clinical translation. Various splitting strategies have been used to improve delivery, but always accompanied by compromised efficiency. Here, we developed a modular and efficient SunTag-PE system that splits...
Zinc-finger nucleases (ZFNs), transcription activator-like endonucleases (TALENs), and CRISPR-associated Cas9 are three major generations of genome editing tools. However, no parallel comparison about the efficiencies off-target activity has been reported, which is critical for final clinical decision. We first time developed genome-wide unbiased identification double-stranded breaks enabled by sequencing (GUIDE-seq) method in ZFNs TALENs with novel bioinformatics algorithms to evaluate...
A genome editing tool targeting the high-risk human papillomavirus (HPV) oncogene is a promising therapeutic strategy to treat HPV-related cervical cancer. To improve gene knockout efficiency, we developed chain reaction (GKCR) method for continually generating mutagenic disruptions and used this disrupt HPV18 E6 E7 genes. We verified that GKCR Cas9/guide RNA (gRNA) cassettes could integrated into targeted loci via homology-independent insertion (HITI). The qPCR results revealed enabled...
CRISPR-associated (Cas) nucleases are multifunctional tools for gene editing. Cas12a possesses several advantages, including the requirement of a single guide RNA and high fidelity Here, we tested three orthologs from human gut samples identified LtCas12a that utilizes TTNA protospacer adjacent motif (PAM) distinct canonical TTTV PAM but with equivalent cleavage ability specificity. These features significantly broadened targeting scope family. Furthermore, developed sensitive, accurate,...
Integration of oncogenic DNA viruses into the human genome is a key step in most virus-induced carcinogenesis. Here, we constructed virus integration site (VIS) Atlas database, an extensive collection breakpoints for three prevalent oncoviruses, papillomavirus, hepatitis B virus, and Epstein-Barr based on next-generation sequencing (NGS) data, literature, experimental data. There are 63,179 47,411 junctional sequences with full annotations deposited VIS comprising 47 genotypes 17 disease...
Abstract Background Hypertrophic cardiomyopathy (HCM) is a prevalent genetic heart disease caused by variants in sarcomere genes like MYH7, which accounts for 40% of cases with 75% being heterozygous. HCM shows incomplete penetrance, where mutant allele expression correlates severity. Thus, allele-specific suppression potential therapy. While RNAi or CRISPR/Cas9 showed moderate efficacy, low level MYH7 associated the risk failure and dilated cardiomyopathy. We hypothesized CRISPR-based...
Abstract Prime editing (PE) holds tremendous potential in the treatment of genetic diseases because it can install any desired base substitution or local insertion/deletion. However, full-length PE effector size (6.3-kb) was beyond packaging capacity adeno-associated virus (AAV), hindering its clinical transformation. Various splitting strategies have been used to improve delivery, but always accompanied by compromised efficiency. Here, we developed a modular and efficient SunTag-PE system...
Here, we present a protocol for generating LtCas12a protein recognizing distinct TTNA (N represented A, T, C, G) protospacer adjacent motif sequence. We describe steps transforming and harvesting bacterial cells purification including nickel affinity chromatography dialysis. then detail procedures verification of with cis- trans-cleavage activities. For complete details on the use execution this protocol, please refer to Chen et al. (2023).1
PET imaging is effective in aiding doctors cancer diagnosis and treatment by reflecting the metabolic rate of glucose human body. However, segmentation remains a challenging task due to its typically low resolution. In this paper, we propose geodesic prior adapted for overcome disadvantages resolution blurred boundaries images.Additionally, design dual-branch network framework that constructs an attention mechanism using prior, effectively fusing features images. Furthermore, introduce...
The PET/MR system provides both anatomical and functional information, which offers much sensitivity accuracy for clinical imaging research. This may lead to certain health risks of radiation exposure the cost radiopharmaceuticals patients, results in low-dose PET reducing dose recovering noisy images. Considering profitable information MR images, we introduce spatial brain alignment images solve denoising tasks imaging. In this paper, a novel 3D network model equipped with transform is...
The axial field of view (FOV) a positron emission tomography (PET) scanner greatly affects the quality PET images. total-body (uEXPLORER) is highermore sensitive but more expensive and difficult to widely use. This study used deep learning utilize high-quality (HQ) images generated by uEXPLORER optimize image short-axis scanners while controlling costs. HQ brain, lungs, abdomen were quantitatively qualitatively compatible with reference In particular, overall mean SSIM between was greater...
Epidermal growth factor receptor (EGFR) is one of the most common driver genes in non-small cell lung cancer (NSCLC), including EGFR-resistance mutations and EGFR-sensitizing mutations. Meanwhile, only NSCLC patients with exhibit a positive response to tyrosine kinase inhibitors (TKIs). Radiomics-based deep learning methods provide noninvasive technologies for predicting patients. In our work, we fused multimodal radiomics data from 18F-FDG PET/CT images algorithm predict Specifically, 202...