A5079565931- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Calcium signaling and nucleotide metabolism
- Endoplasmic Reticulum Stress and Disease
- Erythrocyte Function and Pathophysiology
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Toxoplasma gondii Research Studies
- Cell Image Analysis Techniques
- Photoreceptor and optogenetics research
- Lysosomal Storage Disorders Research
- Photosynthetic Processes and Mechanisms
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)
2023
Center for Nanotechnology Innovation
2017-2022
Scuola Normale Superiore
2020-2022
Italian Institute of Technology
2017-2018
National Enterprise for NanoScience and NanoTechnology
2018
Istituto Nanoscienze
2018
Here we provide demonstration that image mean square displacement (iMSD) analysis is a fast and robust platform to address living matter dynamic organization at the level of sub-cellular nanostructures (e.g. endocytic vesicles, early/late endosomes, lysosomes), with no a-priori knowledge system, need extract single trajectories. From each iMSD, unique triplet average parameters (namely: diffusivity, anomalous coefficient, size) are extracted represented in 3D parametric space, where...
Abstract Time-lapse optical microscopy datasets from living cells can potentially afford an enormous amount of quantitative information on the relevant structural and dynamic properties sub-cellular organelles/structures, provided that both spatial temporal dimensions are properly sampled during experiment. Here we provide exemplary live-cell, time-lapse confocal imaging corresponding to three structures endo-lysosomal pathway, i.e. early endosomes, late endosomes lysosomes, along with...
We investigated lysosome dynamics during neuronal stem cell (NSC) differentiation by two quantitative and complementary biophysical methods based on fluorescence: imaging-derived mean square displacement (iMSD) single-particle tracking (SPT). The former extracts the average size of whole population moving lysosomes directly from imaging, with no need to calculate single trajectories; latter resolves finest heterogeneities dynamic features at single-lysosome level, which are lost in iMSD...
Here we provide demonstration that fast fluorescence fluctuation spectroscopy is a and robust approach to extract information on the dynamics of molecules enclosed within subcellular nanostructures (e.g., organelles or vesicles) which are also moving in complex cellular environment. In more detail, Raster Image Correlation Spectroscopy (RICS) performed at timescales (i.e., microseconds) reveals motion fluorescently labeled two exemplary dynamic biomedical interest, lysosome insulin secretory...
Abstract Exit from the endoplasmic reticulum is mediated by Sar1/COPII machinery and a number of accessory factors. How initial steps cargo recruitment upstream are remains unclear, but dihydropyridine FLI-06 inhibits into ER exit sites. Here, we used chemical genetics screening approaches in conjunction with treatment identified membrane proteins YIPF5 GOT1A/B as putative components early export processes. Surprisingly, two homologous GOT1A GOT1B, coded GOLT1A GOLT1B , respectively,...