A5034392602- Cholinesterase and Neurodegenerative Diseases
- Computational Drug Discovery Methods
- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Bone health and treatments
- Cancer-related gene regulation
- RNA modifications and cancer
- Nicotinic Acetylcholine Receptors Study
- Bone Metabolism and Diseases
- Bone health and osteoporosis research
- Phosphodiesterase function and regulation
- Alzheimer's disease research and treatments
- Molecular Sensors and Ion Detection
- Celiac Disease Research and Management
- Analytical Chemistry and Chromatography
- Chemical synthesis and alkaloids
- Supramolecular Chemistry and Complexes
- Epilepsy research and treatment
- Microscopic Colitis
- Congenital heart defects research
- Crystallography and molecular interactions
- Diet, Metabolism, and Disease
- Pesticide Exposure and Toxicity
- Lymphoma Diagnosis and Treatment
- Cleft Lip and Palate Research
University Hospital Hradec Králové
2015-2024
Charles University
2015-2022
Abstract Background: Aberrant hypermethylation of tumour suppressor genes (TSGs) occurring in hepatocellular carcinoma (HCC) could provide a mean molecular characterisation this cancer. The aim study was to investigate promoter methylation and gene expression selected TSGs HCC identify candidate for further validation as potential biomarkers. Methods: Methylation-specific multiplex ligation-dependent probe amplification method used measure the status 25 49 samples 36 corresponding...
Antidotes against organophosphates often possess physicochemical properties that mitigate their passage across the blood–brain barrier. Cucurbit[7]urils may be successfully used as a drug delivery system for bisquaternary oximes and improve central nervous targeting. The main aim of these studies was to elucidate relationship between cucurbit[7]uril, oxime K027, atropine, paraoxon define potential risks or advantages this in complex vivo system. For reason, silico (molecular docking combined...
Ovarian cancer is the leading cause of death from gynaecologic tumours, but molecular and especially epigenetic events underlying transformation are poorly understood. Various methylation changes have been identified show promise as potential biomarkers. The aim this study was to investigate promoter selected tumour suppressor genes in ovarian by comparison with normal tissue. To search for we used methylation-specific multiplex ligation-dependent probe amplification compare status 44 tissue...
The objective of this study was to elucidate the pharmacokinetics and metabolite formation newly developed non-selective AChE/BChE 7-MEOTA-donepezil-like inhibitors for potential therapeutic use in Alzheimer's disease (AD) patients. chemical structures metabolites were defined during incubation with human liver microsomes, subsequently, metabolization verified vivo study. In vitro metabolic profiling revealed nine major case PC-37 eight PC-48. Hydroxylation enzymatic hydrolysis bonds close...
Epigenetic changes are considered to be a frequent event during tumour development. Hypermethylation of promoter CpG islands represents an alternative mechanism for inactivation suppressor genes, DNA repair cell cycle regulators and transcription factors. The aim this study was investigate methylation specific genes in samples sinonasal carcinoma by comparison with normal tissue. To search epigenetic events we used methylation-specific multiplex ligation-dependent probe amplification...
Our study aimed to investigate the effect of mirtazapine on bone metabolism in orchidectomized rat model.Rats were divided into three groups. A sham-operated control group (SHAM group) and a after orchidectomy (ORX received standard laboratory diet (SLD). An experimental MIRTA SLD enriched with for 12 weeks. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry. marker concentrations osteoprotegerin (OPG), amino-terminal propeptide procollagen type I, alkaline...
Abstract Alzheimer's disease (AD) is a neurodegenerative disorder characterized by memory loss and behavioral psychological symptoms of dementia (BPSD). Given that cholinergic neurons are predominantly affected in AD, current treatments primarily aim to enhance neurotransmission. However, imbalances other neurotransmitters, such as γ‐aminobutyric acid (GABA), also contribute AD symptomatology. In the presented research, using combination crystallography computational methods we developed...
Abstract Alzheimer's disease (AD) is a neurodegenerative disorder characterized by memory loss and behavioral psychological symptoms of dementia (BPSD). Given that cholinergic neurons are predominantly affected in AD, current treatments primarily aim to enhance neurotransmission. However, imbalances other neurotransmitters, such as γ‐aminobutyric acid (GABA), also contribute AD symptomatology. In the presented research, using combination crystallography computational methods we developed...
To date, there is not generally accepted and universal indicator of activity, functional integrity the small intestine in patients with coeliac disease. The aim our study was to investigate whether serum concentrations non-essential amino acids citrulline ornithine might have this function.We examined a subgroup proven disease healthy controls (blood donors).A total 94 (29 men, mean age 53 ± 18 years; 65 women, 44 14 years) 35 donors) whom serologically excluded (10 51 25 46 12 were included...