Mesenchymal stem cells (MSCs) with their multilineage developmental plasticity comprise a promising tool for regenerative cell-based therapy. Despite important biological properties, which the MSCs from different sources share, differences between them are poorly understood. Hence, it is required to assign molecular signature each of these MSC populations, based on cell related genes and early lineage or markers. Understanding propensity differentiate lineages fundamental development...

Multipotent mesenchymal stromal cells (MSCs) from Wharton's jelly (WJ) of umbilical cord bear higher proliferation rate and self-renewal capacity than adult tissue-derived MSCs are a primitive cell population.Stem niche or physiological microenvironment plays crucial role in maintenance stem properties oxygen concentration is an important component the niche.Low tension hypoxia prevalent embryonic many at early stages development.Again, vivo, known to home specifically hypoxic events...

Mesenchymal stromal/stem cells (MSCs) for clinical use have largely been isolated from the bone marrow, although isolation of these many different adult and fetal tissues has reported as well. One such source MSCs is Whartons Jelly (WJ) umbilical cord, it provides an inexhaustible stem potential therapeutic use. Isolation cord also presents little, if any, ethical concerns, process obtaining tissue relatively simple with appropriate consent donor. However, a great majority studies rely on...

We used cre/loxP-based genetic lineage tracing analysis to test a previously proposed hypothesis that in vitro cultured adult pancreatic beta-cells undergo epithelial-mesenchymal transition (EMT) generate highly proliferative, differentiation-competent population of mesenchymal islet "progenitor" cells. Our results the mouse are likely be directly relevant human system show do not EMT and cells arise cultures pancreas derived from beta-cells. argue these most originate expansion integral...

The plus-sense RNA genome of Japanese encephalitis virus (JEV) contains noncoding regions (NCRs) 95 and 585 bases at its 5' 3' ends, respectively. last 83 nucleotides the 3'-NCR are predicted to form stable stem-loop (SL) structures. shape this 3'-SL structure is highly conserved among divergent flaviviruses even though only small stretches nucleotide sequence contained within these structures conserved. These SL have been function as cis-acting signals for replication such may bind viral...

Strategies designed to produce functional cells from stem or mature hold great promise for treatment of different cell-degenerative diseases. Type 1 and type 2 diabetes are examples such Although in origin, both involve inadequate cell mass insulin-producing beta cells, the most abundant pancreatic islets Langerhans. Practical realization strategies is highly dependent on elucidation physiological mechanisms responsible generation new pancreas, which at this time poorly defined. The vitro...

We report the synthesis and dynamical behavior of a carbon dot (CD) with near 100% photoluminescence quantum yield in water for very large pH range (1-12). This CD exhibits rotational correlational time only ∼130 ps, signifying whole is not exhibiting photoluminescence. Unlike most carbon-based nanoparticles (which act as quencher fluorescence), this could donor, Förster model account experimental observables resonance energy transfer (RET) experiment quite well. Based on two measurements,...

Abstract: Mesenchymal stem cells (MSCs) have become an attractive tool for tissue engineering and targets in clinical transplantation due to their regeneration potential immuno-suppressive capacity. Although MSCs derived from bone marrow are the most widely used, harvest requires invasive procedure. The umbilical cord, which is discarded at birth, can provide inexhaustible source of therapy. Wharton's jelly-derived (WJ-MSCs), been shown faster proliferation rates greater expansion capability...

Abstract Background Due to their immunomodulatory and trophic support functions, mesenchymal stem cells (MSCs) are promising in the field of cell-based regenerative medicine. However, MSC survival post-transplantation is challenged by various microenvironment stress factors. Here, we investigated role vitronectin (VTN) strategy MSCs under serum deprivation condition. Methods Proliferation kinetics cell adhesion were determined from population doublings cell-matrix de-adhesion studies,...

Abstract Mesenchymal stem cells (MSCs) are known to facilitate angiogenesis and promote neo-vascularization via secretion of trophic factors. Here, we explored the molecular mechanism adopted by ADAMTS13 in modulating expression some key angiogenic markers human umbilical cord-derived MSCs under serum-deprivation stress. Wharton’s jelly (WJ-MSCs) were isolated from perivascular region cords explant culture. was upregulated at both mRNA protein levels WJ-MSCs Correspondingly, also seen be...

Contrary to the belief of chemistry community, intense charge transfer is observed between meta-oriented donor–acceptor moieties in ultrasmall single-benzenic fluorophores. Red emission any solvent has so far been reported with molecular fluorophores having lowest weight (MW) 252.5 Da; however, we have achieved same a meta-fluorophore an MW only 203.1 Da. nonpolar fluorophore minimum 464.5 Da, but 255.3 Intense Stokes (260 nm) and solvatochromic (160 shifts, high magnitudes fluorescence...

The efficacy of mesenchymal stem cell (MSC) therapy is currently limited by low retention and poor survival transplanted cells as demonstrated clinical studies. This mainly due to the harsh microenvironment created oxygen nutrient deprivation inflammation at injured sites. choice MSC source could be critical in determining fate cellular function MSCs under stress. Our objective here was investigate influence ischemia-like stress on Wharton’s jelly (WJ-MSCs) from human umbilical cord assess...

The smallest fluorophore reported in literature to emit the red has a MW > 250 Da. Hereby, we report unique way obtain red, orange emission ultrasmall fluorophores (DEAMB, = 207 Da), (DEAB, 177 respectively. In this exceptional concept, explore charge transfer between meta-oriented donor–acceptor moieties single-benzenic (meta-fluors). Unlike existing red-emitting small-fluorophores, our meta-fluors exhibit an intense Stokes shift (225 nm), solvatochromic (175 and excited-state lifetime (21...

Application of pancreatic islet transplantation to treatment diabetes is severely hampered by the inadequate supply. This problem could in principle be overcome generating cells from adult pancreas vitro. Although it possible obtain replicating cultures pancreas, these cells, when significantly expanded vitro, progressively lose pancreatic-specific gene expression, including that a "master" homeobox transcription factor Pdx1. Here we show for first time long-term proliferating...

It is the promise of regeneration and therapeutic applications that has sparked an interest in mesenchymal stem cells (MSCs). Following infusion, MSCs migrate to sites injury or inflammation by virtue their homing property. To exert optimal clinical benefits, systemically delivered need efficiently adequate numbers pathological areas vivo. However, underlying molecular mechanisms responsible for MSC migration are still not well understood. The Wharton's jelly (WJ) umbilical cord attractive...

Abstract Mesenchymal stromal cells (MSCs) are clinically beneficial for regenerative treatment of chronic inflammation and autoimmune disorders. However, to attain maximum efficacy from the transplanted MSCs, evaluation its interaction with microenvironment, becomes critical. Fever being an important hallmark inflammation, we investigated effect febrile temperature stress on adhesion migration umbilical cord-derived MSCs. 40 °C-exposure altered cellular morphology significant cell...

Mesenchymal stem cells (MSCs) are successful for their therapeutic application in immune and inflammatory contexts due to anti-inflammatory, trophic, immunomodulatory roles. However, though MSCs have the potential provide regenerative treatment toward a wide range of devastating diseases, massive cell death transplanted remains an obstacle overcome. The relation between inflammation is multifactorial challenging comprehend. Fever critical component inflamed microenvironment. Also, choice MSC...

Mesenchymal stem cells (MSCs), also referred to as "medicinal signaling cells," have gained prominence candidates for cell-based therapy and in clinical trials owing their regenerative therapeutic properties. Here, we present a protocol isolating MSCs from the decidua basalis layer of human placenta using an explant culture approach. We describe steps collecting, disinfecting, plating placental tissue. then detail procedures characterizing isolated through flow cytometry vitro differentiation.