A5066179223- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Amino Acid Enzymes and Metabolism
- Cancer, Hypoxia, and Metabolism
- Microbial Community Ecology and Physiology
- Bacterial Genetics and Biotechnology
- Sulfur Compounds in Biology
- Marine and coastal ecosystems
- Chromium effects and bioremediation
- Enzyme Structure and Function
- Arsenic contamination and mitigation
- Advanced biosensing and bioanalysis techniques
- Advanced Thermodynamics and Statistical Mechanics
- Metal Extraction and Bioleaching
- biodegradable polymer synthesis and properties
- Forest, Soil, and Plant Ecology in China
- Thermochemical Biomass Conversion Processes
- Combustion and flame dynamics
- Mitochondrial Function and Pathology
- Advanced Nanomaterials in Catalysis
- Algal biology and biofuel production
- RNA and protein synthesis mechanisms
- Biochemical Acid Research Studies
- Polyamine Metabolism and Applications
Shandong University
1999-2024
State Key Laboratory of Microbial Technology
2017-2024
Qingdao Binhai University
2024
Second Hospital of Shandong University
2020
Shanghai Jiao Tong University
2020
Sulfide (H2S, HS- and S2-) oxidation to sulfite thiosulfate by heterotrophic bacteria, using sulfide:quinone oxidoreductase (SQR) persulfide dioxygenase (PDO), has recently been reported as a possible detoxification mechanism for sulfide at high levels. Bioinformatic analysis revealed that the sqr pdo genes were common in sequenced bacterial genomes, implying may have other physiological functions. SQRs previously classified into six types. Here we grouped PDOs three types showed some...
Summary Some heterotrophic bacteria are able to oxidize sulfide (H 2 S, HS − and S 2− ) sulfite thiosulfate via polysulfide. The genes coding for the oxidation enzymes in Cupriavidus pinatubonensis JMP134 have recently been identified; however, their regulation is unknown. A regulator gene adjacent operon of sulfide‐oxidizing genes, encoding a σ 54 ‐dependent transcription factor (FisR) with three domains: an R domain, AAA+ domain DNA‐binding domain. Here it reported that responds presence...
Pseudomonas aeruginosa PAO1, an opportunistic human pathogen, deploys several strategies to resist antibiotics. It uses multidrug efflux pumps, including the MexAB-OprM pump, for antibiotic resistance, and it also produces hydrogen sulfide (H2 S) that provides some defense against MexR functions as a transcriptional repressor of mexAB-oprM operon. responds oxidative stresses caused by exposure, displays growth phase-dependent derepression However, intrinsic inducer has not been identified....
Sustainable and environment-friendly microbial fermentation processes have been developed to produce numerous chemicals. However, the high energy input required for sterilization substantial fresh water consumption restrict economic feasibility of traditional processes. To address these problems, Vibrio natriegens, a promising chassis with low nutritional requirements, salt tolerance rapid growth rate can be selected as host chemical production. In this study, V. natriegens was metabolic...
We establish a new pathway of sulfide oxidation with thiosulfate as key intermediate in Cupriavidus pinatubonensis JMP134. The bacterium mainly oxidizes by using sulfide:quinone oxidoreductase, persulfide dioxygenase, and the Sox system intermediate. Although purified reconstituted sulfide, its rate C. JMP134 is too low to be physiologically relevant. findings reveal how these sulfur-oxidizing enzymes participate single bacterium.
ABSTRACT Production of sulfide (H 2 S, HS − , and S 2− ) by heterotrophic bacteria during aerobic growth is a common phenomenon. Some with sulfide:quinone oxidoreductase (SQR) persulfide dioxygenase (PDO) can oxidize self-produced to sulfite thiosulfate, but other without these enzymes release into the medium, from which H volatilize gas phase. Here, we report that Cupriavidus necator H16, fccA fccB genes encoding flavocytochrome c dehydrogenases (FCSDs), also oxidized S. A mutant in were...
Abstract Background Whey is a major pollutant generated by the dairy industry. To decrease environmental pollution caused industrial release of whey, new prospects for its utilization need to be urgently explored. Here, we investigated possibility using whey powder produce 2,3-butanediol (BDO), an important platform chemical. Results Klebsiella oxytoca strain PDL-0 was selected because ability efficiently BDO from lactose, fermentable sugar in whey. After deleting genes pox , pta frdA ldhD...
Acetoin is a potential platform compound for variety of chemicals. Bacillus licheniformis MW3, thermophilic and generally regarded as safe (GRAS) microorganism, can produce 2,3-butanediol with high concentration, yield, productivity. In this study, B. MW3 was metabolic engineered acetoin production. After deleting two dehydrogenases encoding genes budC gdh, an strain (ΔbudCΔgdh) constructed. Using fed-batch fermentation (ΔbudCΔgdh), 64.2 g/L produced at productivity 2.378 g/[L h] yield 0.412...
Eutrophication and deoxygenation possibly occur in coastal waters due to excessive nutrients from agricultural aquacultural activities, leading sulfide accumulation. Cyanobacteria, as photosynthetic prokaryotes, play significant roles carbon fixation the ocean. Although some cyanobacteria can use electron donor for photosynthesis under anaerobic conditions, little is known on how they interact with aerobic conditions. In this study, we report that
Abstract Corn cob is a major waste mass-produced in corn agriculture. hydrolysate containing xylose, arabinose, and glucose the hydrolysis product of cob. Herein, recombinant Escherichia coli strain BT-10 was constructed to transform into 1,2,4-butanetriol, platform substance with diversified applications. To eliminate catabolite repression enhance NADPH supply for alcohol dehydrogenase YqhD catalyzed 1,2,4-butanetriol generation, ptsG encoding transporter EIICB Glc pgi phosphoglucose...
Selective detection of l-lactate levels in foods, clinical, and bacterial fermentation samples has drawn intensive attention. Many fluorescent biosensors based on non-stereoselective recognition elements have been developed for lactate detection. Herein, the allosteric transcription factor STLldR from
Co-combustion characteristics of two coals and biomass are investigated through thermogravimetric method in O2/CO2 atmosphere. Characteristic parameters coal combustion deduced from TG-DTG curves show that, at the O2 concentration 20%, substitution CO2 for N2 brings about delay ignition, exaltation burnout, drop integration parameter. To improve performances atmosphere, is added to form co-combustion. The difficulty atmosphere can be effectively reduced a co-combustion ratio 20%. Activation...
Pyruvate is an important platform material widely used in food, pharmaceutical, and chemical industries. Pyruvate-tolerant Klebsiella oxytoca PDL-0 was chosen as a chassis for pyruvate production via metabolic engineering. Genes related to by-product generation were knocked out decrease the of 2,3-butantediol, acetate, ethanol, succinate. The NADH oxidase encoding gene nox inserted into locus lactate dehydrogenase ldhD genome K. simultaneously block regenerate NAD+. importers CstA YjiY...
l-2-Hydroxyglutarate (l-2-HG) is a functionally compartmentalized metabolite involved in various physiological processes. However, its subcellular distribution and mitochondrial transport remain unclear owing to technical limitations. In the present study, an ultrasensitive l-2-HG biosensor, sfLHGFR
Acetate is an important chemical feedstock widely applied in the food, and textile industries. It now mainly produced from petrochemical materials through processes. Conversion of lignocellulose biomass to acetate by biotechnological pathways both environmentally beneficial cost-effective. However, production carbohydrate hydrolysate via glycolytic involving pyruvate decarboxylation often suffers carbon loss results low yield.
3,4-Dihydroxybutyric acid (3,4-DHBA) is an important platform chemical with versatile applications. Traditional approaches for 3,4-DHBA production involving hazardous materials and harsh reaction conditions are environmentally unfriendly. The reported biotechnological routes rely on microbial monocultures low productivity yield. In this study, a coculture system to synthesize from xylose was established using Gluconobacter oxydans Escherichia coli. First, it confirmed that G. 621H can...
Glycerol is a readily available and inexpensive substance that mostly generated during biofuel production processes. In order to ensure the viability of industry, it essential develop complementing technologies for resource utilization glycerol. Ethylene glycol two-carbon organic chemical with multiple applications huge market. this study, an artificial enzymatic cascade comprised alditol oxidase, catalase, glyoxylate/hydroxypyruvate reductase, pyruvate decarboxylase lactaldehyde:propanediol...
l-Serine is an important platform chemical with wide applications. Fermentative synthesis of l-serine suffers from cytotoxicity and undesired degradation l-serine. Here, we designed in vitro enzymatic cascade for production glycerol, unavoidable byproduct the biofuel industry. NAD+-independent d-lactate dehydrogenase Escherichia coli MG1655 (EcDLD) was found by screening to have high oxidase activity toward d-glycerate. This enzyme thus implemented into accomplish generation hydroxypyruvate...
Production of d -3,4-dihydroxybutyric acid by using an NAD + and ATP free in vitro cascade with four enzymes from -xylose, the predominant sugar hemicellulosic biomass.
Recently, an in vitro enzymatic cascade was constructed to transform glycerol into the high-value platform chemical pyruvate. However, low activity of dihydroxy acid dehydratase from Sulfolobus solfataricus (SsDHAD) limited efficiency. In this study, reduction pyruvate catalyzed by d-lactate dehydrogenase Pseudomonas aeruginosa PAO1 used assay activities dehydratases. Dihydroxy Paralcaligenes ureilyticus (PuDHT) identified as most efficient candidate for glycerate dehydration. After...