Genome editing tools have rapidly been adopted by plant scientists for crop improvement. using a multiplex sgRNA-CRISPR/Cas9 genome system is useful technique improvement in monocot species. In this study, we utilized precise gene techniques to generate wheat 3'(2'), 5'-bisphosphate nucleotidase (

Lead pollution of the environment poses a major global threat to ecosystem. Bacterial bioremediation offers promising alternative traditional methods for removing these pollutants, that are often hindered by various limitations. Our research focused on isolating lead-resistant bacteria from industrial wastewater generated heavily lead-containing industries. Eight strains were successfully isolated, and subsequently identified through molecular analysis. Among these, Enterobacter kobei FACU6...

Glyphosate is a commonly used organophosphate herbicide that has an adverse impact on humans, mammals and soil microbial ecosystems. The redundant utilize of glyphosate to control weed growth cause the pollution environment by this chemical. discharge in agricultural drainage can also serious environmental damage water problems. Therefore, it important develop methods for enhancing degradation through bioremediation. In study, thirty bacterial isolates were selected from agro-industrial zone...

Abstract Isolation of heavy metals-resistant bacteria from their original habitat is a crucial step in bioremediation. Six lead (Pb) resistant bacterial strains were isolated and identified utilizing 16S rRNA to be Enterobacter ludwigii FACU 4, Shigella flexneri FACU, Microbacterium paraoxydans Klebsiella pneumoniae subsp. pneumonia Raoultella planticola 3 Staphylococcus xylosus FACU. It was determined that all these had Minimum inhibitory concentration (MIC) 2500 ppm except R. has higher...

In order to improve the culturability and biomass production of rhizobacteria, we previously introduced plant-only-based culture media. We herein attempted widen scope plant materials suitable for preparation chemically analyzed refuse turfgrass, cactus, clover. They were sufficiently rich support good in vitro growth by rhizobacteria isolates representing Proteobacteria Firmicutes. also adequate efficient produce a cell liquid batch cultures. These media as sufficient artificial cultivation...

The sugarcane (Saccharum X officinarum) is one of the most important crops used to produce sugar and raw material for biofuel in world. One main causes sucrose content yield losses attack by insect. In this investigation, cry1Ac gene was introduced into variety GT54-9(C9) using Agrobacterium tumefaciens transformation method transgenic production presenting insect-resistance. A. strain GV1303 including pARTcry1Ac vector transformed sugarcane. Bacillus thuringiensis cry were successfully...

The plant growth regulator gibberellic acid (GA3) is widely used in agriculture many countries. However, little known about its danger to human health or physiologic and biochemical pathways. Our study examined the effect of GA3 on liver kidney function quercetin hepatorenal toxicity induced by four groups male albino rats. For 4 weeks, control group (CNT) received saline, (QR) daily intraperitoneal injections (50 mg/kg/BW) dissolved (55 via oral gavage, protective was injected with gavaged...

Strawberry (Fragaria × ananassa) is an economically important soft fruit crop with polyploid genome which makes the breeding of new cultivars difficult. Simple and efficient method for transformation regeneration required improvement in strawberry. In present study, adventitious shoot has been investigated three cultivated strawberry plants, i.e., Festival, Sweet Charly Florida via direct organogenesis using vitro juvenile leaves as explants. Explants were collected after sub-culturing on a...

The phenylurea herbicides are persistent in soil and water, necessitating the creation of methods for removing them from environment. This study aimed to examine microbial diversity, searching local bacterial isolates able efficiently degrade herbicide isoproturon, 3-(4-isopropylphenyl)-1, 1-dimethylurea (IPU). best effectively IPU were selected, characterized, identified as Pseudomonas putida Acinetobacter johnsonii. catechol 1, 2-dioxygenase enzyme's catA gene was amplified, cloned,...

Plant regeneration protocols for sugarcane GT54-9(C9) cultivar were developed direct organogenesis and indirect somatic embryogenesis, using young leaf segments as explants by studying the influence of different concentrations types cytokinin auxin hormones. For callus formation from leaves, a medium containing 4mg/l 2,4-D was found very effective. embryo formation, MS supplemented with 1mg/l Kin 0.5 mg/l used. While in case protocol, BAP 2mg/l NAA best shoot formation. Data showed that...

Isoprene represents a key building block for the production of valuable materials such as latex, synthetic rubber or pharmaceutical precursors and serves basis advanced biofuel production. To enhance volatile natural hydrocarbon isoprene, released by plants, animals bacteria, Kudzu isoprene synthase (kIspS) gene has been heterologously expressed in Bacillus subtilis DSM 402 licheniformis 13 using pHT01 vector. As control, heterologous expression KIspS E. coli BL21 (DE3) with pET28b vector...

Abstract Apigenin is one of the most studied flavonoids and widely distributed in plant kingdom. exerts important antioxidant, antibacterial, antifungal, antitumor activities, anti-inflammatory effects neurological or cardiovascular disease. Chalcone isomerase A ( chiA ) an enzyme flavonoid biosynthesis pathway. In order to enhance apigenin production, petunia chi gene was transformed for Astragalus trigonus . Bialaphos survived plants were screened by PCR, dot blot hybridization RT-PCR...

Five bacterial strains were isolated from the hemocoel of greater wax moth larvae (Galleria mellonella) infected with entomopathogenic nematodes: Heterorhabditis bacteriophora HP88, indicus RM1 and sp (S1), Steinernema abbasi sp. (S II). Strains identified as Photorhabdus luminescens HRM1, P. HS1, Xenorhabdus indica X. nematophila ATTC19061 using 16S rDNA sequence analysis. To reveal genetic diversity among these strains, three molecular markers (RAPD, ISSR SRAP) employed. RAPD analysis...

Background and Objective: Bacteriocin produced by lactic acid bacteria has received more scientific attention, this is due to its ability inhibit wide range of spoilage pathogenic microorganisms their potential usefulness as natural substitute for chemical food preservatives.The aim the present study isolation, identification improvement Lactic Acid Bacteria (LAB) that produce bacteriocin from different traditional dairy products in Egypt.Methodology: Isolation LAB isolates based on...

<title>Abstract</title> Sesame (Sesamum indicum L.) is one of the oldest cultivated oil crops worldwide and struggles with low yield, which could be attributed to capsule dehiscence seed shattering just before during full maturation. The present study addresses seed-shattering in sesame via downregulating endo-polygalacturonase (endo-PG) gene activity “known as ripening enzyme”. Five lines plants using an RNAi transformation strategy a non-tissue culture-based technique. Individual...

To achieve the nutritional target of human food, boron (B) has been described as an essential mineral in determining seed and theoretical oil yield Sesamum indicum L. The research to increase its cultivation is garnering attention due high content, quality utilization for various purposes, which include nutrition well use food industry. For this, a two-year field experiment was performed at PAU, Punjab, India determine effect different concentrations foliar-applied B (20, 30 40 mg L-1)...

The root system is the important organ of a plant, helping to anchor plant and take up nutrients from soil. purpose this investigation was determine magnitude network (RNS) through phenotypic variability in broad range maize inbred lines. GiA Root software used identify attributes images. After germination, lines were grown hydroponically for 15 days high-lux growth room with low phosphorus (LP) normal (NP) treatments. Variance analysis revealed large present among lines, intermediate high...

Tomato (Solanum lycopersicum L.) yield is severely affected by Fusarium fungal disease. To improve the resistance of tomato against oxysporum f. sp. lycopersici (FOL), Escherichia coli katE gene was transformed into two cultivars, namely Castle Rock and Super strain B, via Agrobacterium tumefaciens; transformation efficiency 5.6% 3.5%, respectively. The integration T0, T1, T2 transgenic lines confirmed using PCR. In addition, DNA dot blot technique lines. RT-PCR analysis that could be...

Soil acidification and charland formation through alluvial sand deposition are emerging threats to food security in Bangladesh that they endanger crop production about 35% of its territory. The integrated plant nutrient system (IPNS) is a globally accepted management approach designed revive the damaged soils’ fertility level. Total organic carbon (TOC) soil composite index quality has consequences for agricultural productivity natural ecosystems. This study assesses impacts using biochar,...

Plant molecular farming (PMF) is an important growing prospective approach in plant biotechnology; it includes production of recombinant pharmaceutical and industrial proteins large quantities from engineered plants. Elastin a major protein component tissues that require elasticity, helps keep skin smooth as stretches to allow normal. used raw material for the cosmetic industry. In this work, we aimed use bioreactor expression full human tropoelastin protein. Agrobacterium- mediated...

Squash leaf curl virus (SqLCV) is a bipartite begomovirus affecting squash plants. It transmitted by whitefly Bemisia tabaci biotype B causing severe curling, vein banding, and molting ending stunting. In this study full-length genomic clone of SqLCV Egyptian isolated posttranscriptional gene silencing (PTGS) has been induced to develop resistance. The Noubaria more than 95% homology with Jordon, Israel, Lebanon, Palestine, Cairo isolates. Two genes fragment from introduced in sense...