A5003377727- Aquaculture disease management and microbiota
- Aquaculture Nutrition and Growth
- Invertebrate Immune Response Mechanisms
- Genomics and Phylogenetic Studies
- Crustacean biology and ecology
- Animal Nutrition and Physiology
- Meat and Animal Product Quality
- Enzyme Catalysis and Immobilization
- HIV/AIDS drug development and treatment
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Biochemical and Molecular Research
- Marine Bivalve and Aquaculture Studies
- Coastal wetland ecosystem dynamics
- Gut microbiota and health
- Oil Palm Production and Sustainability
- Entomopathogenic Microorganisms in Pest Control
- Pharmacological Receptor Mechanisms and Effects
- Effects of Environmental Stressors on Livestock
- Identification and Quantification in Food
- Livestock and Poultry Management
- Forest Ecology and Biodiversity Studies
- Lichen and fungal ecology
- Biotin and Related Studies
National Center for Genetic Engineering and Biotechnology
2012-2025
National Science and Technology Development Agency
2010-2025
Martin Luther University Halle-Wittenberg
2015
Helmholtz Centre for Environmental Research
2015
To salvage marine ecosystems from fishery overexploitation, sustainable and efficient aquaculture must be emphasized. The knowledge obtained available genome sequence of organisms has accelerated in many cases. black tiger shrimp (Penaeus monodon) is one the most prominent cultured penaeid shrimps (Crustacean) with an average annual global production half a million tons last decade. However, its currently assemblies lack contiguity completeness required for accurate annotation due to highly...
Development of white striping (WS) and wooden breast (WB) in broiler meat have been linked to hypoxia, but their etiologies are not fully understood. This study aimed at investigating absolute expression hypoxia-inducible factor-1 alpha subunit (HIF1A) genes involved stress responses muscle repair using a droplet digital polymerase chain reaction. Total RNA was isolated from pectoralis major collected male 6-week-old medium (carcass weight ≤ 2.5 kg) heavy > broilers. Samples were classified...
Abstract Microbial colonization is an essential process in the early life of animal hosts—a crucial phase that could help influence and determine their health status at later stages. The establishment bacterial community a host has been comprehensively studied many models; however, knowledge on associated with stages Penaeus monodon (the black tiger shrimp) still limited. Here, we examined structures four (nauplius, zoea, mysis postlarva) two shrimp families using 16S rRNA amplicon...
Microorganisms residing in the rumens of cattle represent a rich source lignocellulose-degrading enzymes, since their diet consists plant-based materials that are high cellulose and hemicellulose. In this study, metagenomic library was constructed from buffalo rumen contents using pCC1FOS fosmid vector. Ninety-three clones pooled approximately 10,000 showed degrading activity against AZCL-HE-Cellulose, whereas four other AZCL-Xylan. Contig analysis pyrosequencing data derived selected...
This study aimed at investigating white striping (WS) and wooden breast (WB) cases in meat collected from commercial broilers.A total of 183 samples were male Ross 308 broilers slaughtered the age 6 weeks (n = 100) 7 83). The breasts subjected to defect inspection, quality determination histology evaluation.Of 183, 4 6-week-old classified as non-defective while others exhibited WS lesion. Among birds, defective medium size birds (carcass weight ≤2.5 kg) showed mild moderate degree with no...
Wooden breast (WB) abnormality adversely impacts the quality of chicken meat and has been linked with oxidative stress. In this study, samples were taken from carcasses 7-week-old Ross 308 broilers 20-min 24-h postmortem. Five WB seven non-WB control assigned based on palpatory hardness (non-WB = no unusual characteristics focal or diffused hardness). exhibited lower contents protein amino acids, i.e., isoleucine, leucine valine, lighter surface color, shear force, greater drip loss altered...
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2019 has posed a serious threat to global health and the economy for over two years, prompting need development antiviral inhibitors. Due its vital role viral replication, RNA-dependent RNA polymerase (RdRp) is promising therapeutic target. Herein, we analyzed amino acid sequence conservation RdRp across coronaviruses. conserved acids at catalytic binding site served as ligand-contacting residues silico...
The enzymatic resolution of chiral sofosbuvir precursors is a critical step in producing stereoisomerically pure ProTide drugs, essential for their therapeutic efficacy. In this study, mutated phosphotriesterase (W131M-PTE) was immobilized onto various polymeric macroporous beads, including commercial immobead 150P (IB), modified (IB-EDA and IB-MTD), synthetic polyacrylamide (PAM) beads functionalized with glutaraldehyde (PAM-GA) or 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide (PAM-EDC)....
β-glucans supplements are known for enhancing disease resistance and performance in shrimp aquaculture, but their effectiveness depends on source structure. This study investigated a novel β-glucan derived from the insect fungus Ophiocordyceps dipterigena BCC 2073 as potential feed additive Pacific white (Penaeus vannamei). To determine its feasibility, juvenile shrimps were fed diets supplemented with 0%, 0.1%, 0.4% oligosaccharide 30 days prior to evaluation. The groups supplementation...
A chiral pool synthesis was developed to obtain all four stereoisomeric 2-methyl-3-(4-phenylbutyl)tetrahydro-3-benzazepin-1-ols 21, 31, and 32 in a seven- eight-step sequence. The phenols reveal slightly higher GluN2B affinity than the methyl ethers 21. increases order (1R,2S) < (1S,2S) (1S,2R) (1R,2R). (R,R)-32 (S,R)-32 show highest cytoprotective activity. Both compounds represent selective allosteric NMDA receptor antagonists. Docking of 3-benzazepin-1-ols into ifenprodil binding site...
Understanding the correlation between shrimp growth and their intestinal bacteria would be necessary to optimize animal's performance. Here, we compared bacterial profiles along with shrimp's gene expression responses metabolites in intestines Top Bottom weight groups. Black tiger (Penaeus monodon) were collected from same population rearing environments. The two groups, Top-weight group an average of 36.82 ± 0.41 g Bottom-weight 17.80 11.81 g, selected. Intestines aseptically subjected...
Forage preservation for livestock feeding is usually done by drying the plant material and storing it as hay or ensiling into silage. During process, pH in system lowered activities of lactic acid-producing bacteria (LAB), inhibiting growth spoilage microorganisms maintaining quality ensiled product. To improve this inoculation LAB could be used starter cultures to shorten time control fermentation process. Here, we compared bacterial dynamics two materials, whole-plant corn (Zea mays L.)...
In this work, a metagenomic library was generated from peat-swamp forest soil obtained Narathiwat Province, Thailand. From fosmid of approximately 15,000 clones, six independent clones were found to possess lipolytic activity at acidic pH. Analysis pyrosequencing data revealed ORFs, which exhibited 34–71% protein similarity known lipases/esterases. A clone, designated LP8, demonstrated the highest level under conditions and extracellular activity, subsequently subcloned sequenced. The...
Marine organisms are important to global food security as they the largest source of animal proteins feeding mankind. Genomics-assisted aquaculture can increase yield while preserving environment ensure sufficient and sustainable production for security. However, only few high-quality genome sequences marine organisms, especially shellfish, available public partly because difficulty in sequence assembly due complex nature their genomes. A key step a successful sequencing is preparation high...
DATA REPORT article Front. Mar. Sci., 24 March 2020Sec. Marine Molecular Biology and Ecology https://doi.org/10.3389/fmars.2020.00172
With the advantages that long-read sequencing platforms such as Pacific Biosciences (Menlo Park, CA, USA) (PacBio) and Oxford Nanopore Technologies (Oxford, UK) (ONT) can offer, various research fields genomics transcriptomics exploit their benefits. Selecting an appropriate platform is undoubtedly crucial for success of outcome, thus there a need to compare these evaluate them specific questions. This study aims performance PacBio ONT transcriptomic analysis by utilizing transcriptome data...
Transcriptomes associated with wooden breast (WB) were characterized in broilers at two different market ages. Breasts (Pectoralis major) collected, 20-min postmortem, from male Ross 308 slaughtered 6 and 7 weeks of age. The breasts classified as "non-WB" or "WB" based on palpation hardness scoring (non-WB = no abnormal hardness, WB consistently hardened). Total RNA was isolated 16 samples (n 3 for week non-WB, n WB; 5 WB). Transcriptome profiled using a chicken gene expression microarray...
Specific stereoisomer is paramount as it vital for optimizing drug efficacy and safety. The quest the isolation of desired active pharmaceutical ingredients or key intermediates drives innovation in synthetic biocatalytic methods. Chiral phosphoramidate an important building block synthesis antiviral drugs such remdesivir sofosbuvir. Given clinical potency (
Development of the white striping (WS) abnormality adversely impacts overall quality broiler breast meat. Its etiology remained unclear. This study aimed at exploring transcriptional profiles skeletal muscles exhibiting different WS severity to elucidate molecular mechanisms underlying development and progression WS. Total RNA was isolated from pectoralis major male 7-week-old Ross 308 broilers. The samples were classified as mild (n = 6), moderate 6) or severe 4), based on number thickness...
Abstract With the rapid growth in global demand, shrimp industry needs integrated approaches for sustainable production. A high-quality larva is one of crucial key requirements to maximize Survival and rates during larval development are often criteria evaluate quality, however many aspects gene regulation have not yet been identified. To further our understanding biological processes their early life, transcriptomic analysis developmental stages (nauplius, zoea, mysis, postlarva) were...