A5077774919- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Esophageal Cancer Research and Treatment
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Forensic Entomology and Diptera Studies
- Integrated Circuits and Semiconductor Failure Analysis
- Non-Destructive Testing Techniques
- Insect and Pesticide Research
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Fuel Cells and Related Materials
- Ultrasonics and Acoustic Wave Propagation
- Gastric Cancer Management and Outcomes
- Neuroblastoma Research and Treatments
- Insect behavior and control techniques
- Nanopore and Nanochannel Transport Studies
Cancer Research Institute Ghent
2020-2024
Ghent University
2019-2024
VIB-UGent Center for Medical Biotechnology
2023-2024
Ghent University Hospital
2021-2024
Vlaams Instituut voor Biotechnologie
2023
University of Central Lancashire
2018
Extracellular RNA (cell-free RNA; exRNA) from blood-derived liquid biopsies is an appealing, minimally invasive source of disease biomarkers. As pre-analytical variables strongly influence exRNA measurements, their reporting essential for meaningful interpretation and replication results. The aim this review was to chart what extent are documented, pinpoint shortcomings improve future reporting. In total, 200 blood plasma studies published in 2018 or 2023 were reviewed annotation 22...
Abstract Circulating nucleic acids in blood plasma form an attractive resource to study human health and disease. Here, we applied mRNA capture sequencing of cell-free RNA from 266 cancer patients cancer-free controls (discovery n = 208, 25 types; replication 58, 3 types). We observed type-specific as well pan-cancer alterations transcriptomes compared controls. Differentially abundant RNAs were heterogenous among cohorts, hampering identification robust biomarkers. Therefore, developed a...
<title>Abstract</title> Using blood-based extracellular RNA (circulating cell-free RNA; exRNA) as a clinical biomarker requires validated procedures for sample collection and processing quantification. No study to date has systematically tested pre-analytical variables affecting transcriptome-wide exRNA analysis. We evaluated compared ten blood tubes, three time intervals between draw processing, eight purification methods using the supplier-specified minimal maximal biofluid input volumes....
Blowfly species of the family Calliphoridae can be used in forensic investigations to estimate minimum post-mortem interval (PMImin). Lucilia caesar and illustris (Diptera: Calliphoridae) are closely related phenotypically similar, making reliable identification difficult. To identify potential genetic markers distinguish these species, five complete mitochondrial genomes were sequenced: three for L. (KM657111–KM657113) two (KM657109, KM657110). The ND6 gene contained most species-specific...
In the past decades, incidence of esophageal adenocarcinoma has increased dramatically in Western populations. Better understanding disease etiology along with identification novel prognostic and predictive biomarkers are urgently needed to improve dismal survival probabilities. Here, we performed comprehensive RNA (coding non-coding) profiling various samples from 17 patients diagnosed adenocarcinoma, high-grade dysplastic or non-dysplastic Barrett's esophagus. Per patient, a blood plasma...
Abstract The use of blood-based extracellular RNA (cell-free RNA; exRNA) as clinical biomarker requires the implementation a validated procedure for sample collection, processing, and profiling. So far, no study has systematically addressed pre-analytical variables affecting transcriptome analysis exRNAs. In exRNAQC study, we evaluated ten blood collection tubes, three time intervals between draw downstream eight purification methods using supplier-specified minimum maximum biofluid input...
<title>Abstract</title> The use of blood-based extracellular RNA (cell-free RNA; exRNA) as clinical biomarker requires the implementation a validated procedure for sample collection, processing, and profiling. So far, no study has systematically addressed pre-analytical variables affecting transcriptome analysis exRNAs. In exRNAQC study, we evaluated ten blood collection tubes, three time intervals between draw downstream eight purification methods using supplier-specified minimum maximum...
Abstract Background Blood plasma, one of the most studied liquid biopsies, contains various molecules that have biomarker potential for cancer detection, including cell-free DNA (cfDNA) and RNA (cfRNA). As vast majority nucleic acids in circulation are non-cancerous, a laboratory workflow with high detection sensitivity tumor-derived is prerequisite precision oncology. One way to meet this requirement by combined analysis cfDNA cfRNA from same biopsy sample. So far, no study has...
Abstract Cancer biomarker studies require procedures that provide accurate and precise test results with high analytical sensitivity. Consequently, the growing use of extracellular RNA from human biofluids as clinically relevant requires implementation benchmarked methods for sample collection, processing, profiling. While several small-scale have pointed at impact individual preanalytical factors, no comprehensive study has addressed many variables affecting downstream sequencing...