A5013343993- Microbial Metabolic Engineering and Bioproduction
- Gene expression and cancer classification
- Bacterial Genetics and Biotechnology
- Molecular Biology Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Catalysis and Immobilization
- Genetics, Bioinformatics, and Biomedical Research
- Biosensors and Analytical Detection
- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Machine Learning and Data Classification
- Biofuel production and bioconversion
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Structure and Function
- Protein purification and stability
- Bioinformatics and Genomic Networks
- Mitochondrial Function and Pathology
- Metabolomics and Mass Spectrometry Studies
- Fungal and yeast genetics research
University of Hagen
2021-2023
Rohm (Germany)
2019
University of Cologne
2008-2010
ABSTRACT The metabolism of monocarboxylic acids is central importance for bacteria in their natural habitat as well during biotechnological production. Although biosynthesis and degradation are understood, the transport such compounds still a matter discussion. Here we present identification characterization new system Corynebacterium glutamicum with high affinity acetate propionate lower pyruvate. Biochemical analysis this acid transporter (MctC) revealed first time quantitative...
ABSTRACT Many bacteria can utilize C 4 -carboxylates as carbon and energy sources. However, Corynebacterium glutamicum ATCC 13032 is not able to use tricarboxylic acid cycle intermediates such succinate, fumarate, l -malate sole Upon prolonged incubation, spontaneous mutants which had gained the ability grow on could be isolated. DNA microarray analysis showed higher mRNA levels of cg0277, subsequently was named dccT , in than wild type, transcriptional fusion revealed that a point mutation...
Transporters of the dicarboxylate amino acid-cation symporter family often mediate uptake C(4)-dicarboxylates, such as succinate or l-malate, in bacteria. A member this family, transporter (DctA) from Corynebacterium glutamicum, was characterized to catalyze C(4)-dicarboxylates succinate, fumarate, and which inhibited by oxaloacetate, 2-oxoglutarate, glyoxylate. DctA activity not affected sodium availability but dependent on electrochemical proton potential. Efficient growth C. glutamicum...
Biotherapeutics may contain a multitude of different post-translational modifications (PTMs) that need to be assessed and possibly monitored controlled ensure reproducible product quality. During early development biotherapeutics, unexpected PTMs might prevented by in silico identification characterization together with further molecular engineering. Mass determinations human IgG1 (mAb1) bispecific IgG-ligand fusion protein (BsAbA) demonstrated the presence unusual resulting major +80 Da,...
Genetics plays an ever-increasing role in medical diagnostics. The requirements for laboratory diagnostics are constantly changing due to new emerging diagnostic procedures, methodologies, devices, and regulatory requirements. Standard software already available laboratories often cannot keep up with the latest developments or is focused on research rather than process automation. Although utilized subject requirements, there no well-defined formal procedure development. Reference models...
Genomic applications like gene expression analysis or metagenomics are increasingly useful tools for laboratory diagnostics as they provide biomarkers physiological and pathophysiological states. Artificial intelligence can help to analyze, visualize interpret results obtained by genomic instruments. However, most software solutions the of data not suitable use face several legal technical challenges. Therefore, we propose a conceptual architecture named "GenDAI" that combines artificial...
Genomic data enable the development of new biomarkers in diagnostic laboratories. Examples include from gene expression analyses or metagenomics. Artificial intelligence can help to analyze these data. However, laboratories face various technical and regulatory challenges harness Existing software for genomic is usually designed research does not meet requirements use as a tool. To address challenges, we recently proposed conceptual architecture called “GenDAI”. An initial evaluation...
Mitochondrial DNA (mtDNA) damage is closely associated with typical diseases of aging, such as Alzheimer’s or Parkinson’s disease, and other health conditions, infertility. This manifests in reduced mitochondrial copy number deletion mutations mtDNA. Consequently, the analysis by determining parameters ratio using quantitative real-time PCR (qPCR) interest for clinical diagnostics. To bring findings from research into laboratory practice, a suitable reliable process needed, which must be...