A5024230254- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Bacterial Genetics and Biotechnology
- Microbial Natural Products and Biosynthesis
- Synthesis and Biological Activity
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Viral Infectious Diseases and Gene Expression in Insects
- Biochemical and Molecular Research
- Fermentation and Sensory Analysis
- Alkaloids: synthesis and pharmacology
- Carbohydrate Chemistry and Synthesis
- Plant biochemistry and biosynthesis
- Plant Disease Resistance and Genetics
- Cancer Research and Treatments
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Microbial Community Ecology and Physiology
- CRISPR and Genetic Engineering
- Microbial bioremediation and biosurfactants
- Metabolism and Genetic Disorders
University of Stuttgart
2014-2023
Bielefeld University
2010-2014
Heinrich Heine University Düsseldorf
2008
We recently engineered Corynebacterium glutamicum for aerobic production of 2-ketoisovalerate by inactivation the pyruvate dehydrogenase complex, pyruvate:quinone oxidoreductase, transaminase B, and additional overexpression ilvBNCD genes, encoding acetohydroxyacid synthase, isomeroreductase, dihydroxyacid dehydratase. Based on this strain, we C. isobutanol from glucose under oxygen deprivation conditions l-lactate malate dehydrogenases, implementation ketoacid decarboxylase Lactococcus...
ABSTRACT Many bacteria can utilize C 4 -carboxylates as carbon and energy sources. However, Corynebacterium glutamicum ATCC 13032 is not able to use tricarboxylic acid cycle intermediates such succinate, fumarate, l -malate sole Upon prolonged incubation, spontaneous mutants which had gained the ability grow on could be isolated. DNA microarray analysis showed higher mRNA levels of cg0277, subsequently was named dccT , in than wild type, transcriptional fusion revealed that a point mutation...
Transporters of the dicarboxylate amino acid-cation symporter family often mediate uptake C(4)-dicarboxylates, such as succinate or l-malate, in bacteria. A member this family, transporter (DctA) from Corynebacterium glutamicum, was characterized to catalyze C(4)-dicarboxylates succinate, fumarate, and which inhibited by oxaloacetate, 2-oxoglutarate, glyoxylate. DctA activity not affected sodium availability but dependent on electrochemical proton potential. Efficient growth C. glutamicum...
In this study, we demonstrate that sigma(E), an alternative sigma factor of Corynebacterium glutamicum, is involved in cell surface stresses. Cells which the sigE gene was deleted evidenced increased sensitivity to magnesium deficiency, as well SDS, lysozymes, EDTA and heat. We utilized physiological analyses show downstream gene, designated cseE, encodes anti-sigma factor. The retarded growth cseE mutant cells under ordinary conditions could be recovered by additional deletion encoding...
Corynebacterium glutamicum is able to grow with lactate as sole or combined carbon and energy source. Quinone-dependent L-lactate dehydrogenase LldD known be essential for utilization of by C. glutamicum. D-lactate also serves source ATCC 13032.Here, the gene cg1027 was shown encode quinone-dependent (Dld) enzymatic analysis protein purified from recombinant E. coli. The absorption spectrum Dld indicated presence FAD bound cofactor. Inactivation dld resulted in loss ability D-lactate, which...
Abstract To build or dissect complex pathways in bacteria and mammalian cells, it is often necessary to recur at least two plasmids, for instance harboring orthogonal inducible promoters. Here we present SiMPl, a method based on rationally designed split enzymes intein-mediated protein trans -splicing, allowing the selection of cells carrying plasmids with single antibiotic. We show that, compared traditional antibiotics, SiMPl increases production antimicrobial non-ribosomal peptide...
Streptogramins are the last line of defense antimicrobials with pristinamycin as a representative substance used therapeutics against highly resistant pathogenic bacteria. However, emergence (multi)drug-resistant pathogens renders these valuable antibiotics useless; making it necessary to derivatize compounds for new compound characteristics, which is often difficult by chemical de novo synthesis due complex nature molecules. An alternative derivatization mutasynthesis. Herein, we report...
The non-proteinogenic aromatic amino acid, p-amino-L-phenylalanine (L-PAPA) is a high-value product with broad field of applications. In nature, L-PAPA occurs as an intermediate the chloramphenicol biosynthesis pathway in Streptomyces venezuelae. Here we demonstrate that model organism Escherichia coli can be transformed metabolic grafting approaches to result improved producing strain.Escherichia K-12 cells were genetically engineered for production from glycerol main carbon source. To do...
Abstract L-phenylglycine (L-Phg) is a rare non-proteinogenic amino acid, which only occurs in some natural compounds, such as the streptogramin antibiotics pristinamycin I and virginiamycin S or bicyclic peptide antibiotic dityromycin. Industrially, more interesting than L-Phg enantiomeric D-Phg it plays an important role fine chemical industry, where used precursor for production of semisynthetic β-lactam antibiotics. Based on operon from Streptomyces pristinaespiralis stereo-inverting...
Aromatic amines are an important class of chemicals which used as building blocks for the synthesis polymers and pharmaceuticals. In this study we establish a de novo pathway biosynthesis aromatic para-amino phenylethanol (PAPE) para-amino-phenylacetic acid (4-APA) in Escherichia coli. We combined synthetic para amino-L-phenylalanine with fungal Ehrlich pathway. Therefore overexpressed heterologous genes encoding 4-amino-4-deoxychorismate synthase (pabAB from Corynebacterium glutamicum),...