A5028557730- Advanced Proteomics Techniques and Applications
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Mass Spectrometry Techniques and Applications
- Autophagy in Disease and Therapy
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Silkworms and Sericulture Research
- Agricultural Practices and Plant Genetics
- Nuclear Physics and Applications
- Bacterial biofilms and quorum sensing
- Clostridium difficile and Clostridium perfringens research
- Genetics and Plant Breeding
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- Escherichia coli research studies
- Enzyme Structure and Function
- Protein purification and stability
- Bacterial Identification and Susceptibility Testing
- Evolution and Genetic Dynamics
- RNA modifications and cancer
- Studies on Chitinases and Chitosanases
- Genomics and Phylogenetic Studies
Navsari Agricultural University
2023
Washington University in St. Louis
2019-2022
University of Houston
2015-2021
Significance Proteins are the machinery which execute essential cellular functions. However, measuring their abundance within an organism can be difficult and resource-intensive. Cells use a variety of mechanisms to control protein synthesis from mRNA, including short open reading frames (uORFs) that lie upstream main coding sequence. Ribosomes preferentially translate uORFs instead sequence, leading reduced translation protein. In this study, we show uORF sequence variation between...
Dormancy is a protective state in which diverse bacteria, including Mycobacterium tuberculosis, Staphylococcus aureus, Treponema pallidum (syphilis), and Borrelia burgdorferi (Lyme disease), curtail metabolic activity to survive external stresses, antibiotics. Evidence suggests dormancy consists of continuum interrelated states, viable but nonculturable (VBNC) persistence states. VBNC contribute antibiotic tolerance, reemergence from latent infections, even quorum sensing biofilm formation....
We have engineered the substrate specificity of chymotrypsin to cleave after Asn by high-throughput screening large libraries created comprehensive remodeling binding pocket. The variant (chymotrypsiN, ChyB-Asn) demonstrated an altered with expanded preference for Asn-containing substrates. confirmed that protein engineering did not compromise stability enzyme biophysical characterization. Comparison wild-type ChyB and ChyB-Asn in profiling lysates HEK293 cells both qualitative quantitative...
Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometers are simple and robust used for analysis of biologically relevant molecules in diverse fields including pathogen identification, imaging spectrometry, natural products chemistry. Despite high nominal resolution accuracy, we have observed significant variability where 30–50% individual replicate measurements errors excess 5 parts-per-million, even when using 5-point internal calibration. Increasing the...
The present research was undertaken to examine the combining ability of 10 parents and 21 hybrids that resulted from crossing seven lines three testers in line × tester mating design, for yield quality governing traits tomato. study indicated no single parent finest combiner all attributes. estimated variance resulting GCA significant fruit per plant girth, suggesting additive gene action responsible these characteristics. SCA observed height, fruits plant, TSS ascorbic acid, manifesting...
Competition assays measure differences between populations of bacteria after stress adaptation, different and mutations in antibiotic resistance genes. We have developed a competition-based assay to evaluate if genes upregulated under starvation are important for bacterial survival. Stress responses critical survival non-pathogenic pathogenic alike including Mycobacterium tuberculosis, Enterococcus fecaelis, Escherichia coli Staphylococcus aureus. Unfortunately, most stress-survival proteins...
Abstract Membrane proteins represent major drug targets, and the ability to determine their functions, structures, conformational changes will significantly advance mechanistic approaches both biotechnology bioremediation, as well fight against pathogenic bacteria. A pertinent example is Mycobacterium tuberculosis (H37Rv), which contains ~4000 protein‐coding genes, with almost a thousand having been categorized ‘membrane protein’, few of (~1%) have functionally characterized structurally...
17Sep 2016 SELECTIVE SEPARATION OF IRON (III) FROM IMMOBILIZATION CATION EXCHANGER RESIN WITH TRIOCTYPHOSPHINE OXIDE SORBENT. S. H. Burungale. A.V. Mali Department of Chemistry, Yashwantrao Chavan College Science, Karad-415124, India.
Abstract The 5’ untranslated region (UTR) sequence of eukaryotic mRNAs may contain upstream open reading frames (uORFs), which can regulate translation the main frame (mORF). current model translational regulation by uORFs posits that when a ribosome scans an mRNA and encounters uORF, uORF prevent ribosomes from reaching mORF cause decreased translation. In this study, we first observed rare variants in UTR dysregulate protein abundance. Upon further investigation, found near start codon...