A5000504868- Tuberculosis Research and Epidemiology
- Immune responses and vaccinations
- Mycobacterium research and diagnosis
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Autophagy in Disease and Therapy
- HIV Research and Treatment
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Bacterial Genetics and Biotechnology
- vaccines and immunoinformatics approaches
- Phagocytosis and Immune Regulation
- Biochemical and Molecular Research
- RNA modifications and cancer
- Preterm Birth and Chorioamnionitis
- RNA Interference and Gene Delivery
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Diagnosis and treatment of tuberculosis
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Mesenchymal stem cell research
- Clostridium difficile and Clostridium perfringens research
- Calcium signaling and nucleotide metabolism
Cornell University
2017-2024
Houston Methodist
2017-2024
Lady Reading Hospital
2024
National JALMA Institute for Leprosy & Other Mycobacterial Diseases
2023
National Chemical Laboratory
2006-2021
Weill Cornell Medicine
2020
Methodist Hospital
2020
The University of Texas Health Science Center at Houston
2011-2019
University of Science and Technology Bannu
2018
Khoula Hospital
2017
Abstract Mycobacterium tuberculosis (Mtb) is responsible for approximately 1.5 million deaths each year. Though 10% of patients develop (TB) after infection, 90% these infections are latent. Further, mice nearly uniformly susceptible to Mtb but their M1-polarized macrophages (M1-MΦs) can inhibit in vitro, suggesting that M1-MΦs may be able regulate anti-TB immunity. We sought determine whether human MΦ heterogeneity contributes TB Here we show IFN-γ-programmed degrade through increased...
Human mesenchymal stem cells (MSCs) express scavenger receptors that internalize lipids, including oxidized low-density lipoprotein (oxLDL). We report MSCs phagocytose Mycobacterium tuberculosis (Mtb) through two types of (SRs; MARCO and SR-B1), as blockade the with antibodies or siRNA knockdown decreased uptake Mtb. also expressed mannose receptor (MR) was found to endocytose rhodamine-labeled mannosylated BSA (rMBSA), though not involved in Dil-oxLDL rMBSA taken up into MSC endosomes...
ABSTRACT Mycobacterium tuberculosis is one of the strongest reducers nitrate among all mycobacteria. Reduction to nitrite, mediated by reductase (NarGHJI) M. , induced during dormant stage, and enzyme has a respiratory function in absence oxygen. Nitrite (NirBD) also functional aerobic growth when nitrite sole nitrogen source. However, role NirBD-mediated reduction dormancy not yet characterized. Here, we analyzed as well hypoxic model vitro . When was used source medium, organism grew...
Abstract Mycobacterium bovis BCG is widely used as a vaccine against tuberculosis due to M. (Mtb), which kills millions of people each year. variably protects children, but not adults tuberculosis. evades phagosome maturation, autophagy, and reduces MHC-II expression antigen-presenting cells (APCs) affecting T-cell activation. To bypass these defects, an autophagy-inducing, TLR-2 activating C5 peptide from Mtb-derived CFP-10 protein was overexpressed in combination with Ag85B. Recombinant...
An improved vaccine is urgently needed to replace the now more than 100-year-old Bacillus Calmette-Guérin (BCG) against tuberculosis (TB) disease, which represents a significant burden on global public health. Mycolic acid, or cord factor trehalose 6,6' dimycolate (TDM), lipid component abundant in cell wall of pathogen Mycobacterium (MTB), has been shown have strong immunostimulatory activity but remains underexplored due its high toxicity and poor solubility. Herein, we employed novel...
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), is the leading cause of death due to bacterial infections in mankind, and BCG, an attenuated strain bovis, approved vaccine. BCG sequesters immature phagosomes antigen presenting cells (APCs), which do not fuse with lysosomes, decreased processing reduced Th1 responses. However, Mtb derived ΔfbpA mutant underwent limited phagosome maturation, enhanced immunogenicity was as effective protecting mice against TB. To facilitate...
Although the BCG vaccine offers partial protection, tuberculosis remains a leading cause of infectious disease death, killing ∼1.5 million people annually. We developed mucosal vaccines expressing autophagy-inducing peptide C5 and mycobacterial Ag85B-p25 epitope using replication-defective human adenovirus (HAdv85C5) bovine (BAdv85C5) vectors. BAdv85C5-infected dendritic cells (DCs) expressed robust transcriptome genes regulating antigen processing compared to HAdv85C5-infected DCs. DCs...
Macrophages (MФ) are an essential immune cell for defense and repair that travel to different tissues adapt based on local stimuli. A critical factor may govern their polarization is the crosstalk between metabolism epigenetics. However, simultaneous measurements of metabolites, epigenetics, proteins (phenotype) have been a major technical challenge. To address this, we developed novel triomics approach using mass spectrometry comprehensively analyze proteins, histone modifications in single...
Interferon (IFN) signaling contributes to stemness, cell proliferation, death, and cytokine in cancer immune cells; however, the role of IFN glioblastoma (GBM) GBM stem-like cells (GSCs) is unclear. Here, we investigated cancer-cell-intrinsic tumorigenesis GBM. We report here that GSCs tumors exhibited differential cell-intrinsic type I II signaling, high IFN/STAT1 was associated with mesenchymal phenotype poor survival outcomes. In addition, chronic inhibition decreased proliferation...
Although classically associated with myelopoiesis, granulocyte-macrophage colony-stimulating factor (GM-CSF) is being increasingly recognized for its potential role in innate resistance against tuberculosis (TB). While the GM-CSF produced by a variety of host cells, including conventional and non-conventional T macrophages, alveolar epithelial cell population that promotes mediated protection Mycobacterium infection remains unclear. This because studies related to so far have been carried...
The dormant phenotype of Mycobacterium tuberculosis that develops during infection poses a major challenge in disease treatment, since these bacilli show tolerance to front-line drugs. An vitro hypoxia dormancy model was established, which produced phenotypically smegmatis after prolonged incubation under conditions low oxygen, pH, and nutrient limitation. Bacilli this displayed the classical characters, including loss acid fastness, altered morphology, and, most importantly, form M. treated...