A5050000313- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Tuberculosis Research and Epidemiology
- Advanced biosensing and bioanalysis techniques
- Mycobacterium research and diagnosis
- Antimicrobial Peptides and Activities
- Phytase and its Applications
- Supramolecular Self-Assembly in Materials
- Bacteriophages and microbial interactions
- Nanopore and Nanochannel Transport Studies
- Ionic liquids properties and applications
- Sphingolipid Metabolism and Signaling
- Cancer therapeutics and mechanisms
- Surfactants and Colloidal Systems
- Catalytic Alkyne Reactions
- Folate and B Vitamins Research
- Nanoparticle-Based Drug Delivery
- Biochemical and Structural Characterization
- Advanced Nanomaterials in Catalysis
- Advanced Chemical Sensor Technologies
- Peptidase Inhibition and Analysis
- HIV/AIDS drug development and treatment
- Antibiotic Resistance in Bacteria
- Sports and Physical Education Research
- Calcium signaling and nucleotide metabolism
RMIT University
2015-2024
MIT University
2015-2020
National Chemical Laboratory
2008-2015
Iowa State University
2005-2006
[reactions: see text] A wide variety of substituted naphthalenes are readily prepared regioselectively under mild reaction conditions by the 6-endo-dig electrophilic cyclization appropriate arene-containing propargylic alcohols ICl, I2, Br2, NBS, and PhSeBr. 3-Iodo-2-naphthols have also been in excellent yields analogous 1-aryl-3-alkyn-2-ones. This methodology accommodates various functional groups has successfully extended to synthesis carbazoles dibenzothiophenes.
Lipid nanoparticles of internal cubic symmetry, termed cuboplexes, are potential nonviral delivery vehicles for gene therapy due to their "topologically active" nature, which may enhance endosomal escape and improve outcomes. In this study, we have used cationic based on monoolein (MO) doped with a lipid, the encapsulation antisense green fluorescent protein (GFP)—small interfering RNA (siRNA) into Chinese Hamster Ovary (CHO)—GFP cells. Agarose gel electrophoresis has confirmed successful...
Antibiotic-resistant bacteria pose a significant threat to humanity. Gram-negative strains have demonstrated resistance last resort antibiotics, partially due their outer membrane, which hinders transport of antimicrobials into the bacterium. Nanocarrier (NC)-mediated drug delivery is one proposed strategy for combating this emerging issue. Here, uptake self-assembled lipid nanocarriers cubic symmetry (cubosomes) revealed fundamental differences in mechanism between Gram-positive and...
Mycobacterium tuberculosis (MTB) causes the infectious disease (TB), responsible for more deaths than any other single in history. Intracellular MTB are slow growing and difficult to target with traditional antitubercular drugs, leading emergence of multidrug resistance TB infection, which is a major global public health issue. Recent advances innovative lipid nanotechnologies drug delivery have demonstrated promising outcomes chronic diseases but not yet been tested as potential systems...
An improved vaccine is urgently needed to replace the now more than 100-year-old Bacillus Calmette-Guérin (BCG) against tuberculosis (TB) disease, which represents a significant burden on global public health. Mycolic acid, or cord factor trehalose 6,6' dimycolate (TDM), lipid component abundant in cell wall of pathogen Mycobacterium (MTB), has been shown have strong immunostimulatory activity but remains underexplored due its high toxicity and poor solubility. Herein, we employed novel...
We report here the lyotropic liquid crystalline phase behavior of two lipid nanoparticulate systems containing mixtures monoolein, capric acid, and saturated diacyl phosphatidylcholines dispersed by Pluronic F127 block copolymer. Synchrotron small-angle X-ray scattering (SAXS) was used to screen a library nanoparticles in high-throughput manner. It found that adding acid had opposing effects on spontaneous membrane curvature monoolein layer hence internal mesophase final nanoparticles. By...
Low discovery rates for new antibiotics, commercial disincentives to invest, and inappropriate use of existing drugs have created a perfect storm antimicrobial resistance (AMR). This "silent pandemic" AMR looms as an immense, global threat human health. In tandem, many potential novel drug candidates are not progressed due elevated hydrophobicity, which may result in poor intracellular internalization undesirable serum protein binding. With reducing arsenal effective enabling technology...
The development of a macrophage-based, antitubercular high-throughput screening system could expedite discovery programs for identifying novel inhibitors. In this study, the kinetics nitrate reduction (NR) by Mycobacterium tuberculosis during growth in Thp1 macrophages was found to be almost parallel viable bacilli count. NR culture medium containing 50 mM optimum on fifth day after infection with M. tuberculosis. signal-to-noise (S/N) ratio and Z-factor obtained from macrophage-based assay...
The bicontinuous lipidic cubic phase (LCP), which is based on the fundamental structure of lipid bilayer, increasingly used in a range applications including drug delivery, meso crystallization membrane proteins, biosensors, and biofuel cells. majority LCPs investigated to date have been formulated from single or combination two lipids water. Such systems lack tunability, with only narrow lattice parameters adopted. In addition, bilayer these materials lacks complexity natural cell...
The potential of gene therapy has not yet been realized, largely due to difficulties in the targeted delivery DNA tissues and cells. Lipid-based nanovectors are use their ability enhance fusion with cellular membranes transport large polyanionic molecules into cytoplasm. While research date mainly focused on liposome-based vectors, recently, nonlamellar phases more complex internal architectures based hexagonal or cubic symmetry have received increasing attention fusogenic properties, which...
The electrostatic interaction between the negatively charged phosphate groups of plasmid DNA and cationic part hydrophobic ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([Bmim][PF6]), initiates spontaneous self-assembly to form functional nanostructures made up (IL). These were demonstrated as promising synthetic nonviral vectors for efficient bacterial pGFP gene transformation in cells. In particular, that 1 μL IL ([Bmim][PF6]) μg can increase efficiency by 300–400% microbial...
Swelling the water channels of lipid-based inverse bicontinuous cubic phases.
Lipid nanoparticles containing a cationic lipid are increasingly used in drug and gene delivery as they can display improved cellular uptake, enhanced loading for anionic cargo such siRNA mRNA or exhibit additional functionality cytotoxicity against cancer cells. This research study tests the hypothesis that molecular structure of influences nanoparticle, resultant cytotoxicity.
Intrinsic therapeutic and biocatalytic roles of ionic liquid mediated self-assembled phytase, platinum–phytase platinum–phytase–curcumin nanospheres.
The success of the lipidic cubic phase for crystallization, particularly integral membrane proteins, is increasing. In past two years, more than 25% protein structures have been solved within biomimetic environment phase. However, relationship between lipid matrix and crystal growth still remains a mystery. Herein we show that bilayer structure crucial to retention functionality dopamine D2 long receptor. Destruction architecture at higher concentrations associated with significant drop in...
Progesterone (PR) is an endogenous steroid hormone that activates the progesterone receptor (PgR) and known to play a critical role in cancer progression. Herein, we report development of cationic lipid-conjugated PR derivatives by covalently conjugating with lipids varying hydrocarbon chain lengths (n = 6-18) through succinate linker. Cytotoxicity studies performed on eight different cell lines reveal PR10, one lead derivatives, exerts notable toxicity (IC50 4-12 μM) cells irrespective...
Antimicrobial resistance (AMR) poses a global health crisis demanding innovative solutions. Traditional antibiotics, though pivotal over the past century in combating bacterial infections, face diminished efficacy against evolving defense mechanisms, especially Gram‐negative strains. This study explores self‐assembled ionizable lipid nanoparticles (LNPs) with incorporation of two components (one cationic, one anionic) nanocarriers for advanced antimicrobial drug delivery broad‐spectrum...
Our inability to completely control TB has been due in part the presence of dormant mycobacteria. This also renders drug regimens ineffective and is prime cause appearance drug-resistant strains. In continuation our efforts develop novel antitubercular agents that especially target mycobacteria, a set 55 new compounds belonging pyrimidone class were designed on basis CoMFA CoMSIA studies, these synthesized subsequently tested against both virulent BCG strain M. tuberculosis. Some have...
We herein report 13 protic ionic liquids (PILs) as tunable solvation media to regulate the internal lyotropic liquid crystalline mesophase of monoolein-based nanoparticles. A range nanostructures, including inverse bicontinuous cubic, hexagonal, and sponge/lamellar mesophases, were produced verified by synchrotron small-angle X-ray scattering. Notably, manipulating cation/anion structures PILs can alter monoolein packing behavior cause a sequential phase transition (hexagonal → cubic...