Abstract Background The host–microbiota interaction plays a crucial role in maintaining homeostasis and disease susceptibility, microbial tryptophan metabolites are potent modulators of host physiology. However, whether how these mediate interactions, particularly terms inter-microbial communication, remains unclear. Results Here, we have demonstrated that indole-3-lactic acid (ILA) is key molecule produced by Lactobacillus protecting against intestinal inflammation correcting dysbiosis....

Inflammatory bowel disease (IBD), including Crohn’s (CD) and ulcerative colitis (UC), is characterized by chronic relapsing inflammation within the gastrointestinal tract. Antibiotics have been used to treat IBD, primarily utilizing metronidazole. Although there does seem be a treatment effect, broad-spectrum antibiotics that date are crude tools many adverse effects. Available evidence suggests host microbiome implicated in pathogenesis of though key bacteria remain unknown. If bacterial...

Abstract Drug delivery with nanocarriers relies on the interaction of individual cell surface. For lipid-based NCs, this uniquely involves a process membrane fusion between lipid bilayer that makes up NC and membrane. Cubosomes have emerged as promising fusogenic however their interactions had not yet been directly observed due to difficulties in achieving adequate resolution or disentangling multiple common characterization techniques. Moreover, many studies these performed under static...

Foods or feedstuffs contaminated by enterotoxigenic Escherichia coli (ETEC) cause severe health problems in human infants and young animals. With the emergence of public concerns rapid spread drug-resistance bacteria, there is extraordinary interest antimicrobial peptides as potential candidates treating pathogen infections. Nature Microcin J25 (MccJ25), a member class lasso isolated from fecal strain E. coli, has been reported to exhibit strong activity against gram-negative bacteria....

The purpose of this study was to investigate the effects antimicrobial peptide microcin J25 (MccJ25) on growth performance, immune regulation, and intestinal microbiota in broilers. A total 3120 one-day-old male Arbor Acres (AA) broilers were randomly allocated five groups (12 replicates, 52 chickens per replicate). treatments control, challenge (0 mg/kg MccJ25), different dosages (AMP) (0.5 1mg/kg antibiotic (20 colistin sulfate). MccJ25 increased body weight gain (starter overall) that...

Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and microbiota. We examined whether L. reuteri I5007 could protect mice against colitis in ameliorating inflammation, modulating microbiota, metabolic composition. In vitro, HT-29 cells were cultured with or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), posttreatment. Pretreatment effectively relieves inflammation challenged...

Ionisable amino-lipid is a key component in lipid nanoparticles (LNPs), which plays crucial role the encapsulation of RNA molecules, allowing efficient cellular uptake and then releasing from acidic endosomes. Herein, we present direct evidence for remarkable structural transitions, with decreasing membrane curvature, including inverse micellar, to hexagonal, two distinct bicontinuous cubic, finally lamellar phase mainstream COVID-19 vaccine ionisable ALC-0315 SM-102 lipids, occurring upon...

Abstract Self‐assembled lipid nanoparticles (LNPs), serving as essential nanocarriers in recent COVID‐19 mRNA vaccines, provide a stable and versatile platform for delivering wide range of biological materials. Notably, LNPs with unique inverse mesostructures, such cubosomes hexosomes, are recognized fusogenic the drug delivery field. This study delves into physicochemical properties, including size, lyotropic liquid crystalline mesophase, apparent pK various components, consisting two...

With oleylamine as a solvent and reducing agent, Au nanoparticles (AuNPs) are grown in situ on graphene oxide (GO) sheets. Thereafter, porous-layered stack of AuNPs–GO nanosheets is formed by gas-phase chemical reduction step. After the AuNPs modified with 11-mercaptoundecanoic acid (11-MUA) to graft –COOH sensing groups amine, functionalized AuNPs–rGO porous-layer stacked loaded onto gravimetric resonant microcantilever for use mass material. The rGO sheets serve multi-layer nano-shelves...

Abstract Composite materials of porous pyrolyzed polyacrylonitrile–sulfur@graphene nanosheet (pPAN–S@GNS) are fabricated through a bottom‐up strategy. Microspherical particles formed by spray drying mixed aqueous colloid PAN nanoparticles and graphene nanosheets, followed simple heat treatment with elemental sulfur. The pPAN–S primary wrapped homogeneously loosely within three‐dimensional network nanosheets (GNS). hierarchical pPAN–S@GNS composite shows high reversible capacity 1449.3 mAh g...

Modulation of the synthesis endogenous host defense peptides (HDPs) by probiotics represents a novel antimicrobial approach for disease control and prevention, particularly against antibiotic-resistant infections in human animals. However, extent HDP modulation is species dependent strain specific. In present study, The porcine small intestinal epithelial cell line (IPEC-J2) cells neonatal piglets were used as in-vitro in-vivo models to test whether Lactobacillus reuteri I5007 could modulate...

Microcin J25 (MccJ25) is an antimicrobial peptide produced by a fecal strain of Escherichia coli containing 21 AA. This study was performed primarily to evaluate the effects MccJ25 as potential substitute for antibiotics (AB) on growth performance, nutrient digestibility, microbiota, and intestinal barrier function in weaned pigs. In present study, 180 pigs (7.98 ± 0.29 kg initial BW) were randomly assigned 1 5 treatments, including basal diet (CON) CON supplemented with AB (20 mg/kg...

Abstract Background The early-life microbiota exerts a profound and lifelong impact on host health. Longitudinal studies in humans have been informative but are mostly based the analysis of fecal samples cannot shed direct light early development mucosa-associated intestinal its GI function. Using piglets as model for human infants, we assess here succession across tract first 35 days after birth. Results Although sharing similar composition predicted functional profile at birth, microbiome...

Abstract Recombinant antimicrobial peptide microcin J25 (MccJ25) causes potent activity against enterotoxigenic Escherichia coli (ETEC) in vitro; however, independently of this activity, its role suppressing intestinal inflammation and epithelial barrier injury vivo remains unclear. We investigated the therapeutic effects MccJ25 on dysfunction underlying mechanism, using gentamicin for comparison. In a mouse model inflammation, administration either or after ETEC K88 infection attenuated...

Abstract Supramolecular complexation is a powerful strategy for engineering materials in bulk and at interfaces. Metal–phenolic networks (MPNs), which are assembled through supramolecular complexes, have emerged as suitable candidates surface particle owing to their diverse properties. Herein, we examine the dynamics of MPNs during thermal transformation processes. Changes local network including enlarged pores, ordered aromatic packing, metal relocation arise from treatment air or an inert...

Multidrug resistant (MDR) bacterial infection has emerged, raising concerns about untreatable infections, and posing the highest health risks. Antimicrobial peptides (AMPs) are thought to be best remedy for this problem. Here, we showed biosynthetic microcin J25 (MccJ25) exhibited excellent bactericidal activity against standard clinically relevant veterinary MDR strains with high stability, no cytotoxicity, increase in drug resistance. Analysis of antimicrobial mechanism possessed by...

Microcin C7 is an antimicrobial peptide produced by Escherichia coli, composed of a heptapeptide with modified adenosine monophosphate. This study was performed to evaluate the effects as potential substrate traditional antibiotics on growth performance, immune functions, intestinal barrier, and cecal microbiota broilers. In current study, 300 healthy Arbor Acres broiler chicks were randomly assigned one five treatments including corn-soybean basal diet supplemented antibiotic or 2, 4, 6...

Glioblastoma multiforme (GBM) is an aggressive brain cancer with high malignancy and resistance to conventional treatments, resulting in a bleak prognosis. Nanoparticles offer way cross the blood-brain barrier (BBB) deliver precise therapies tumor sites reduced side effects. In this study, we developed angiopep-2 (Ang2)-functionalized lipid cubosomes loaded cisplatin (CDDP) temozolomide (TMZ) for crossing BBB providing targeted glioblastoma therapy. Developed showed particle size of around...

Cancer remains a highly lethal disease due to its elusive early detection, rapid spread, and significant side effects. Nanomedicine has emerged as promising platform for drug delivery, diagnosis, treatment monitoring. In particular, carbon dots (CDs), type of fluorescent nanomaterial, offer excellent fluorescence properties the ability carry multiple drugs simultaneously through covalent bonding. this work, CDs with carbonyl groups on surface were prepared by aldol condensation reacted amine...

Methicillin-resistant Staphylococcus aureus (MRSA) is the major pathogen causing serious hospital infections worldwide. With emergence and rapid spread of drug-resistant bacteria, there extraordinary interest in antimicrobial peptides (AMPs) as promising candidates for treatment antibiotic-resistant bacterial infections. Sublancin, a glycosylated AMP produced by Bacillus subtilis 168, has been reported to possess protective activity against infection. This study was performed evaluate...

A universal femtoliter surface droplet-based platform for direct quantification of trace hydrophobic compounds in aqueous solutions is presented. Formation and functionalization droplets, concentrating the analyte solution, are integrated into a simple fluidic chamber, taking advantage long-term stability, large surface-to-volume ratio, tunable chemical composition these droplets. In situ extracted analytes achieved by surface-enhanced Raman scattering (SERS) spectroscopy nanoparticles on...