Abstract Background The host–microbiota interaction plays a crucial role in maintaining homeostasis and disease susceptibility, microbial tryptophan metabolites are potent modulators of host physiology. However, whether how these mediate interactions, particularly terms inter-microbial communication, remains unclear. Results Here, we have demonstrated that indole-3-lactic acid (ILA) is key molecule produced by Lactobacillus protecting against intestinal inflammation correcting dysbiosis....

Obesity, particularly increased visceral fat, positively correlates with various metabolic challenges, including atherosclerosis, but the mechanism is not fully understood. The aim of this study to determine role visceral-fat-derived exosomes (Exo) in endothelial cells and atherosclerosis. We show that obesity changes miRNA profile adipose mice. Importantly, exosomal miR-27b-3p efficiently enters into vascular activates NF-κB pathway by downregulating PPARα. Mechanistically, binds directly...

Abstract Background The immature neonatal fecal microbiota substantially impacts the development of gut health and greatly increases risk disease. Developing effective strategies to modulate has great significance. Herein, we investigated whether maternal dietary supplementation oral administration Lactobacillus reuteri could effectively promote maturation microbiome in piglets from birth weaning. Results Metagenomic analysis colostrum showed that L. influenced overall composition, decreased...

Abstract Feature matching plays a crucial role in computer vision, with applications visual localization, simultaneous localization and mapping (SLAM), image stitching, more. It establishes correspondences between sets of feature points from multiple images, enabling various tasks. Over the years, has witnessed significant development, an increasing number methods being applied. However, different exhibit degrees applicability scenarios requirements due to their rationales. To cope these...

Abstract Background The structural diversity of extracellular polymeric substances produced by microorganisms is attracting particular attention. Poly-gamma-glutamic acid (γ-PGA) a widely studied substance from Bacillus species. function γ-PGA varies with its molecular weight (Mw). Results Herein, different endogenous promoters in licheniformis were selected to regulate the expression levels pgdS , resulting formation Mw values ranging 1.61 × 10 3 2.03 4 kDa. yields and exopolysaccharides...

Free-piston engine coupled with linear generator is a new kind of thermoelectric energy converter. It has been highly concerned due to its high efficiency and low emission in recent years. In this paper, tubular moving-coil developed for the application free-piston engines. The two-dimensional finite-element model established, electromagnetic characteristics are analyzed. A power converter designed control operating state electric current generator. prototype used bore 102 mm, main...

Abstract A novel extracellular polymeric substance (EPS) with flocculating activity produced by Pseudomonas fluorescein isolated from soil was studied in this paper. Firstly, atmospheric and room temperature plasma (ARTP) applied to get a mutant of P. higher EPS production. T4-2 exhibited 106.48% increase compared the original strain. The maximum yield enhanced up 6.42 g/L, nearly 10 times than strain on 3.6-L bioreactor optimized fermentation conditions. Moreover, reached 3023.4 U/mL,...

Abstract Background As a potential source of polyunsaturated fatty acids (PUFA), Schizochytrium sp. has been widely used in industry for PUFA production. Polyketide synthase (PKS) cluster is supposed to be the primary way synthesis one three open reading frames (ORF) PKS cluster, ORFC plays an essential role acid biosynthesis. However, function domains remained unclear. Results In this study, heterologous expression and overexpression were carried out study its accumulation. Firstly, was...

Polysaccharides and poly-γ-glutamic acid (γ-PGA) are biomacromolecules that have been reported as bioflocculants, they exhibit high flocculating activity in many industrial applications. Bacillus licheniformis CGMCC 2876 can produce polysaccharide γ-PGA bioflocculants under different culture conditions. Several key genes involved the metabolic pathway of polysaccharides B. licheniformis, but impacts regulation these on production not illustrated completely. To increase bioflocculant identify...

Abstract Background 5-Aminolevulinic acid (ALA) recently received much attention due to its potential application in many fields. In this study, an ALA production strain of Escherichia coli was constructed by rational metabolic engineering and stepwise improvement based on known regulatory information CRISPR/Cas9 mediated gene knockout. Results A strategy produce directly from glucose recombinant E. via the C5 pathway applied herein. The knockouts significantly improved 662.3 1601.7 mg/L....

We propose a new photo-guided drawing tool for the easy generation of artistic strokes in style flower painting, which is most representative one Chinese paintings, through stylizing rough sketch lines over photoimage. While existing migration methods aim at processing entire image, we depict that stroke-based stylization framework better performs detailed features and catches user's intent. The steps our technique are first to correct user inaccurate mark candidates patterns on painting...

Reproducing the writing process of ancient handwritten artworks is a popular way to appreciating and learning expert skills Chinese calligraphy. This paper presents system for reappearing processes calligraphic characters in different styles. In order convey accurate brush skill inside stroke, character first decomposed into several strokes, then trajectory footprint data each stroke are calculated based on edge skeleton, which reveal relations between shape description skills, finally can...

Generative adversarial networks (GANs) have been used as a solution to handwritten Chinese character automatic generation (HCCAG) in recent years. However, most existing GAN-based methods adopt pixel-based strategy, which ignores the radical structure of characters. To achieve better HCCAG, constraint-based GAN (RC-GAN) is proposed this work. In method, gated recurrent unit (GRU)-based learning network designed calculate components among characters, and consistent loss applied train module....

This paper presents a novel approach for real-time ink wash painting style rendering on geometric models. We first create an footprint model in particle form, then extract classified strokes from ordinary 3D models by utilizing the existing geometry information, and finally combine to generate immersive painted image. By taking advantage of parallel processing GPU, consequently, with realistic features Chinese can be performed consumer-level PC real-time.

Flocculation model of<italic>B. licheniformis</italic>CGMCC 2876 deduced by XDLVO and surface thermodynamic characterization.