A5061258543- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Advanced Drug Delivery Systems
- Drug Transport and Resistance Mechanisms
- Ocular Surface and Contact Lens
- Drug Solubulity and Delivery Systems
- Viral gastroenteritis research and epidemiology
- Advanced biosensing and bioanalysis techniques
- Drug-Induced Hepatotoxicity and Protection
- Pancreatic and Hepatic Oncology Research
- Glaucoma and retinal disorders
- Colorectal Cancer Treatments and Studies
- Advancements in Transdermal Drug Delivery
- Nanoparticle-Based Drug Delivery
- Gastroesophageal reflux and treatments
- Clinical Nutrition and Gastroenterology
- Antibiotics Pharmacokinetics and Efficacy
- 3D Printing in Biomedical Research
- Dialysis and Renal Disease Management
- Diet and metabolism studies
- Pharmacogenetics and Drug Metabolism
- Immunotherapy and Immune Responses
- Gastrointestinal motility and disorders
- Liver physiology and pathology
- Catalytic C–H Functionalization Methods
Nagasaki University
2015-2025
University of Dental Medicine
2009
Nagasaki University Hospital
2005
Nagoya University
2000
Kyoto Pharmaceutical University
1989-1992
Kyoto University
1989-1992
Although new drugs have recently been developed within the field of ophthalmology, eye's various defense mechanisms make it difficult to achieve an effective concentration these eye. Drugs administered systemically poor access inside eye because blood-aqueous and blood-retinal barriers. And although topical instillation is very popular in topically applied are rapidly eliminated from precorneal area. In addition, cornea, considered a major pathway for ocular penetration drugs, barrier drug...
Peritonitis is a serious complication in peritoneal dialysis patients and requires antibiotic administration. Intraperitoneal vancomycin an empiric therapy for peritonitis caused by Gram-positive cocci; however, there no way to predict absorption after intraperitoneal Therefore, we aimed evaluate the changes injection into mice with chlorhexidine gluconate (CG) induced injuries. Additionally, examined correlation between equilibration test (PET) score. PET score was determined using glucose...
Cell-penetrating peptides are powerful tools in the delivery of drugs, proteins, and nucleic acids into cells; therefore, focus has recently been placed on their development. In this study, we synthesized seven types possessing three l-arginines (l-Arg) six l-leucines (l-Leu) and/or 1-aminocyclopentane-1-carboxylic (Ac5c), investigated secondary structures cell-penetrating abilities. The peptide composed an equal number l-Arg, l-Leu, Ac5c formed 310/α-helical TFE solution exhibited highest...
Evaluation methods for determining the distribution of transgene expression in body and vivo fate viral non-viral vectors are necessary successful development gene delivery systems. Here, we evaluated spatial using tissue clearing methods. After hydrodynamic injection plasmid DNA into mice, whole tissues were subjected to clearing. Tissue followed by confocal laser scanning microscopy enabled evaluation three-dimensional without preparation sections. Among tested (ClearT2, SeeDB, CUBIC),...
Purpose. In this study, we developed various ternary complexes of encapsulated polyplexes and lipoplexes using chondroitin sulfate (CS) investigated their universal usefulness for gene delivery. Methods. To prepare the cationic complexes, pDNA was mixed with some vectors such as poly-L-arginine, poly-L-lysine, N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethylammonium chloride (DOTMA)-cholesterol liposomes, DOTMA- dioleylphosphatidylethanolamine (DOPE) liposomes. CS added to constructions...
Typical examples of non-viral vectors are binary complexes plasmid DNA with cationic polymers such as polyethyleneimine (PEI). However, problems cytotoxicity and hemagglutination, owing to their positively charged surfaces, hinder in vivo use. Coating anionic polymers, γ-polyglutamic acid (γ-PGA), can prevent hemagglutination. the role interactions between these serum components gene transfer remains unclear. In this study, we analyzed contribution using PEI/plasmid γ-PGA/PEI/plasmid ternary...
The effects of ophthalmic preservatives on the drug permeability through isolated ocular membranes albino rabbits were investigated using a two-chamber glass diffusion cell. Tilisolol and fluorescein isothiocyanate (FITC)-dextrans (average molecular weights 4400 9400 Da; FD-4 FD-10, respectively) used as model penetrants beta-blockers peptide drugs. Preservatives significantly enhanced corneal penetration not only tilisolol but also FITC-dextrans. Especially, benzalkonium chloride increased...
We have recently discovered the potential for in vivo naked plasmid DNA (pDNA) transfer into gastric serosal surface cells mice. As pDNA are huge molecules, mechanism of gene without carriers and physical forces is great biological interest. The endocytic route mesothelial was not clathrin- or caveolae-mediated endocytosis, but macropinocytosis. Naked required both actin polymerization myosin-based contraction. Upstream kinases Rho family GTPases, Syk, Src PI-3K were involved transfer....
A doxorubicin (Dox) and curcumin (Cur) combination treatment regimen has been widely studied in pre-clinical research. However, the nanoparticles developed for this therapy require a consecutive drug loading process because of different water-solubility these drugs. This study provides strategy "one-step" formation encapsulating both Dox Cur. We took advantage polyacrylic acid (PAA) calcium carbonate (CaCO3) to realise high entrapment efficiency (EE) pH-sensitive release using simplified...
In the delivery of cell-impermeable molecules, cell-penetrating peptides (CPPs) have been attracting increasing attention as intracellular tools. present study, we designed four types cyclic α,α-disubstituted α-amino acids (dAAs) with basic functional groups on their five-membered rings and different chiralities at α-position introduced them into arginine (Arg)-rich peptides. The evaluation abilities indicated that these exhibited better cell permeabilities than an Arg nonapeptide....
Abstract Absorption of organic anions (phenol red, bromphenol blue and bromosulphonphthalein) has been studied after their application to rat liver surface in-vivo, employing a cylindrical glass cell (i.d. 9 mm, area 0·64 cm2). Each drug appeared gradually in the blood with peak level at about 1 h, which its concentration declined slowly. Absorbed model was efficiently excreted into bile. These observations appear indicate possibility absorption from membrane. drugs estimated be more than...