A5049628915- Reproductive tract infections research
- Bacterial Infections and Vaccines
- Syphilis Diagnosis and Treatment
- Sphingolipid Metabolism and Signaling
- Lipid Membrane Structure and Behavior
- Escherichia coli research studies
- Clostridium difficile and Clostridium perfringens research
- Urinary Tract Infections Management
- Gut microbiota and health
- Immune Response and Inflammation
- Toxin Mechanisms and Immunotoxins
- Cytomegalovirus and herpesvirus research
- Surfactants and Colloidal Systems
- Urinary and Genital Oncology Studies
- Streptococcal Infections and Treatments
- Molecular Sensors and Ion Detection
- Reproductive Physiology in Livestock
- Immune cells in cancer
- Gout, Hyperuricemia, Uric Acid
- Porphyrin and Phthalocyanine Chemistry
- Viral gastroenteritis research and epidemiology
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- Reproductive Health and Contraception
- Yersinia bacterium, plague, ectoparasites research
Robert Koch Institute
2013-2024
Max Planck Institute for Infection Biology
2010
Max Planck Society
2008
Chlamydia trachomatis is an important human pathogen that replicates inside the infected host cell in a unique vacuole, inclusion. The formation of this intracellular bacterial niche essential for productive infections. Despite its importance biology, holistic view on protein composition inclusion, including membrane, currently missing. Here we describe cell-derived proteome isolated C. inclusions by quantitative proteomics. Computational analysis indicated inclusion complex trafficking...
Many bacterial pathogens translocate effector proteins into host cells to manipulate cell functions. Here, we used a protein microarray comprising virtually all human SRC homology 2 (SH2) and phosphotyrosine binding domains comprehensively quantitatively assess interactions between the early phase Chlamydia trachomatis translocated actin-recruiting phosphoprotein (Tarp), which is rapidly tyrosine phosphorylated upon entry. We discovered numerous novel SH2 phosphopeptides derived from Tarp....
Abstract Ceramide transfer protein (CERT) mediates non‐vesicular of ceramide from endoplasmic reticulum to Golgi apparatus and thus catalyzes the rate‐limiting step sphingomyelin biosynthesis. Usually, CERT ligands are evaluated in tedious binding assays or non‐homogenous using radiolabeled ceramides. Herein, a facile sensitive assay for CERT, based on Förster resonance energy (FRET), is presented. To this end, we mixed donor acceptor vesicles, each containing different fluorescent species....
ABSTRACT The egress of intracellular bacteria from host cells and cellular tissues is a critical process during the infection cycle. This essential for to spread inside can influence outcome an infection. For obligate Gram-negative zoonotic bacterium Chlamydia psittaci, little known about mechanisms resulting in bacterial infected epithelium. Here, we describe characterize -containing spheres (CCSs), novel predominant type non-lytic utilized by spp. CCSs are spherical, low-phase contrast...
Chlamydia trachomatis is an intracellular pathogen that requires different nutrients, including sphingolipids, for survival. Components the transport and biosynthesis of sphingolipids thus may have a potential as antichlamydial targets. In this study, we synthesized collection 24 ceramide derivatives. Three derivatives show pronounced activity with submicromolar IC50. The potency these compounds was one order magnitude higher than antibiotic chloramphenicol. We detailed structure-activity...
ABSTRACT Chlamydia trachomatis is a medically important human pathogen causing different diseases, including trachoma, the leading cause of preventable blindness in developing countries, and sexually transmitted infections that can lead to infertility ectopic pregnancies. There no vaccine against C. at present. Broad-spectrum antibiotics are used as standard therapy treat infection but have unwanted side effects, such inducing persistent or recurring affecting host microbiome, necessitating...
Chlamydia trachomatis translocates the effector protein Tarp (translocated actin-recruiting phosphoprotein) into host cell cytoplasm where it is quickly tyrosine phosphorylated. Abl and Src kinases have been implicated in phosphorylation; however, we observed that situation more complex. Chemical inhibition of family confirmed a role for these phosphorylation. Infection Src, Yes, Fyn (SYF)-deficient cells showed dampened, but incompletely blocked, Inhibition an SYF background still did not...
Chlamydiaceae are bacterial pathogens that cause diverse diseases in humans and animals. Despite their broad host tissue tropism, all Chlamydia species share an obligate intracellular cycle of development have evolved sophisticated mechanisms to interact with eukaryotic cells. Here, we analysed interactions the zoonotic pathogen psittaci a human epithelial cell line. We found C. recruits ceramide transport protein (CERT) its inclusion. Chemical inhibition CRISPR/Cas9-mediated knockout CERT...
Abstract Within the German Gonococcal Resistance Network’s (GORENET) Neisseria gonorrhoeae (NG) sample collection, azithromycin-resistant NG isolates increased from 4.3% in 2016 to 9.2% 2018. We aim understand this observed increase using whole genome sequencing of combined with epidemiological and clinical data. Reduced susceptibility azithromycin 2018 was predominately clonal (NG multiantigen sequence typing G12302) could mainly be attributed recently described mosaic-like mtr locus. Our...
Fretful novelty: We developed two novel doubly labelled fluorescent ceramide analogues that exhibit significant FRET and undergo hydrolysis by ceramidases. present a sphingolipid probe allows homogeneous ratiometric determination of enzyme activity in real-time. As service to our authors readers, this journal provides supporting information supplied the authors. Such materials are peer reviewed may be re-organized for online delivery, but not copy-edited or typeset. Technical support issues...
We monitored antimicrobial susceptibility developments of Neisseria gonorrhoeae in Germany from January 2014 to May 2021. The proportion isolates with azithromycin minimum inhibitory concentrations above the epidemiological cut-off increased substantially, 1.3% 12.2% 2020. Preliminary data 2021 showed a further rise (January May: 20.7%). Therefore, as part recommended dual therapy for non-adherent patients is challenged. Antimicrobial testing clinical practice crucial and continuous...
Background Emerging antimicrobial resistance (AMR) challenges gonorrhoea treatment and requires surveillance. Aim This observational study describes the genetic diversity of Neisseria gonorrhoeae isolates in Germany from 2014 to 2017 identifies N. multi-antigen sequence typing (NG-MAST) genogroups associated with AMR or some patient demographics. Methods 1,220 gonococcal underwent testing NG-MAST. Associations between sex/age patients were statistically assessed. Results Patients’ median age...
Egress of intracellular pathogens is highly regulated and carefully timed. For the zoonotic bacterium C. psittaci, predominant egress pathway Chlamydia-containing sphere (CCS) formation, a calcium-dependent sequential mechanism including protease activity, inclusion membrane destabilization, calcium increase, plasma blebbing. How to ensure that it takes place only after psittaci development thus far unknown. Here, we show recruits cellular ceramide transporter CERT its during development,...
<h3>Background</h3> Azithromycin resistant Neisseria gonorrhoeae (NG) isolates increased from 4.3% in 2016 to 9.2% 2018 within the German Gonococcal Resistance Network (GORENET) NG sample collection. Using whole genome sequencing (WGS) of combination with clinical and epidemiological data, we aim understand this observed increase. <h3>Methods</h3> GORENET was set up 2013 as a laboratory network monitor infections Germany by collection isolates, data. In 2018, reduced susceptibility...
Abstract Egress of intracellular bacteria from host cells and cellular tissues is a critical process during the infection cycle. This egress essential for to spread inside can influence outcome an infection. For obligate Gram-negative zoonotic bacterium Chlamydia psittaci little known about mechanisms resulting in chlamydial infected epithelium. Here, we describe characterize novel non-lytic pathway C. by formation -containing spheres (CCS). CCS are spherical, low phase contrast structures...
<h3>Background</h3> <i>Neisseria gonorrhoeae</i> (NG) infections are not reportable in Germany. The Gonococcal Resistance Network (GORENET) is a laboratory network to monitor antimicrobial resistance (AMR) Germany, linking data from sequence typing epidemiological data. We described prevalence of gonococcal types Germany and associations AMR improve future treatment prevention strategies. <h3>Methods</h3> NG isolates collected between April 2014 December 2017 were tested by E-test typed...
<h3>Background</h3> German national guidelines recommend ceftriaxone combined with azithromycin for <i>Neisseria gonorrhoeae</i> (NG) treatment since 2014. The Gonococcal-Resistance-Network (GORENET) monitors gonococcal antimicrobial resistance (AMR) in Germany. aim is to assess whether are still effective Germany and which factors affect higher minimum inhibitory concentrations (MICs). <h3>Methods</h3> GORENET laboratories sent NG isolates the conciliar laboratory centralized retesting of...