A5030231061- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- SARS-CoV-2 and COVID-19 Research
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- COVID-19 Clinical Research Studies
- Cancer Immunotherapy and Biomarkers
- Lipid metabolism and biosynthesis
- Diabetes and associated disorders
- Metabolomics and Mass Spectrometry Studies
- Immunotherapy and Immune Responses
- RNA regulation and disease
- Iron Metabolism and Disorders
- Cancer-related gene regulation
- Natural Antidiabetic Agents Studies
- Endoplasmic Reticulum Stress and Disease
- Pharmacology and Obesity Treatment
- vaccines and immunoinformatics approaches
- Heat shock proteins research
- Angiogenesis and VEGF in Cancer
- Molecular Biology Techniques and Applications
Magna Graecia University
2015-2024
German Cancer Research Center
2021
Heidelberg University
2021
Centro di Riferimento Oncologico della Basilicata
2018
Istituti di Ricovero e Cura a Carattere Scientifico
2018
Abstract Remarkable deregulation of microRNAs has been demonstrated in epithelial ovarian cancer (EOC). In particular, some the let-7 miRNA family members have proposed as tumor suppressors. Here, we explored functional roles let-7g EOC. The ectopic overexpression OVCAR3 and HEY-A8 EOC cells induced i) a down-regulation c-Myc cyclin-D2 thus promoting cell cycle arrest, ii) reduction Vimentin, Snail Slug counteracting progression to mesenchymal transition, iii) chemosensitization cis...
Metastases are the major cause of cancer-related morbidity and mortality. By time cancer cells detach from their primary site to eventually spread distant sites, they need acquire ability survive in non-adherent conditions proliferate within a new microenvironment spite stressing that may severely constrain metastatic process. In this study, we gained insight into molecular mechanisms allowing an anchorage-independent manner, regardless both tumor-intrinsic variables nutrient culture...
Breast cancer is the most frequent in women worldwide and late diagnosis often adversely affects prognosis of disease. Radiotherapy commonly used to treat breast cancer, reducing risk recurrence after surgery. However, eradication radioresistant cells, including stem remains main challenge radiotherapy. Recently, lipid droplets (LDs) have been proposed as functional markers also being involved increased cell tumorigenicity. LD biogenesis a multistep process requiring various enzymes,...
Reactive oxygen species (ROS) mediates cisplatin-induced cytotoxicity in tumor cells. However, when ROS do not reach cytotoxic levels, cancer cells may develop chemoresistance. This phenomenon can be attributed to the inherited high expression of antioxidant protein network. H-Ferritin is an important member system due its ability store iron a nontoxic form. Altered has been described ovarian cancers; however, functional role cisplatin-based chemoresistance this type never explored. Here, we...
Abstract Ferritin is a nanocage protein composed by the variable assembly of 24 heavy and light subunits. As major intracellular iron storage protein, ferritin has been studied for many years in context metabolism. However, recent evidences have highlighted its role, particular that subunit (FHC), pathways related to cancer development progression, such as cell proliferation, growth suppressor evasion, death inhibition, angiogenesis. At least partly, involvement these due ability FHC control...
Although much progress has been made in cancer treatment, the molecular mechanisms underlying radioresistance (RR) as well biological signatures of radioresistant cells still need to be clarified. In this regard, we discovered that breast, bladder, lung, neuroglioma, and prostate 6 Gy X-ray resistant were characterized by an increase lipid droplet (LD) number containing highest LDs showed clonogenic potential after irradiation. Moreover, observed LD content was tightly connected with iron...
Ferritin plays a central role in the intracellular iron metabolism; molecule is nanocage of 24 subunits heavy and light types. The subunit (FHC) provided ferroxidase activity thus performs key transformation non-toxic form. Recently, it has been shown that FHC also involved additional not iron-related critical pathways including, among others, p53 regulation, modulation oncomiRNAs expression chemokine signalling. Epithelial to mesenchymal transition (EMT) cellular mechanism by which cell...
In a previous study, we showed that the silencing of heavy subunit (FHC) offerritin, central iron storage molecule in cell, is accompanied by modification global gene expression. this work, explored whether different FHC amounts might modulate miRNA expression levels K562 cells and studied impact miRNAs profile modifications. To aim, performed miRNA-mRNA integrative analysis silenced for (K562shFHC) comparing it with transduced scrambled RNA (K562shRNA). Four miRNAs, namely hsa-let-7g,...
Ferritin, the major iron storage protein, performs its essential functions in cytoplasm, nucleus and mitochondria. The variable assembly of 24 subunits Heavy (H) Light (L) type composes cytoplasmic molecule. In humans, two distinct genes code these subunits, both belonging to complex multigene families. Until now, one H gene has been identified with coding sequence interrupted by three introns more than 20 intronless copies widely dispersed on different chromosomes. Two are actively...
The H Ferritin subunit (FTH1), as well regulating the homeostasis of intracellular iron, is involved in complex pathways that might promote or inhibit carcinogenesis. This function may be mediated by its ability to interact with different molecules. To gain insight into FTH1 interacting molecules, we analyzed interactome HEK293T cells. Fifty-one proteins have been identified, and among them, focused our attention on a member peroxiredoxin family (PRDX6), an antioxidant enzyme plays important...
The cell-microenvironment communication is essential for homing of hematopoietic stem cells in stromal niches. Recent evidences support the involvement epithelial-to-mesenchymal (EMT) process cell homeostasis as well leukemia invasiveness and migration capability. Here, we demonstrate that alteration iron consequent increase redox metabolism, mediated by stable knock down ferritin heavy chain (FtH), enhances expression CXCR4 K562 erythroleukemia cells, thus promoting CXCL12-mediated...
Despite the abundance of studies on beneficial effects a fiber rich diet as well polyphenols deriving from Citrus fruits postprandial serum glucose and insulin, clinical evidence their synergic healthy subjects have never been published. We aimed to investigate feasibility new dietary approach in controlling insulin response at breakfast time by testing brioche enriched with wheat bran bergamot fiber.We enrolled 11 volunteers cross-over study. Participants consumed classic our functional...
Nuclear Factor-κB (NF-κB) is frequently activated in tumor cells contributing to aggressive growth and resistance chemotherapy. Here we demonstrate that Ferritin Heavy Chain (FHC) protein expression inversely correlates with NF-κB activation cancer cell lines. In fact, FHC silencing K562 SKOV3 lines induced p65 nuclear accumulation, whereas overexpression correlated depletion the same FHC-silenced cells, accumulation was reverted by treatment reactive oxygen species (ROS) scavenger,...
Emerging evidence shows that oral squamous cell carcinoma (OSCC) invasiveness can be attributed to a small subpopulation of cancer stem cells (CSCs) in the bulk tumor. However, presence CSCs OSCC close resection margins is still poorly unexplored. Here, we found BMI1, CD44, SOX2, OCT4, UBE2C, CXCR4 marker genes are significantly upregulated, while IGF1-R, KLF4, ALDH1A1, CD133, FAM3C downregulated tumor core vs healthy mucosa 24 patients with OSCC. Among these, SOX2 appears also upregulated...
Abstract Background The evolving variants of SARS-CoV-2 may escape immunity from prior infections or vaccinations. It’s vital to understand how adapts these changes. Both infection and mRNA vaccination induce T cells that target the Spike protein. These can recognize multiple variants, such as Delta Omicron, even if neutralizing antibodies are weakened. However, degree recognition vary among people, affecting vaccine efficacy. Previous studies demonstrated capability T-cell receptor (TCR)...
Introduction: The PD-1/PD-L1 axis is hijacked by lung adenocarcinoma (LUAD) cells to escape immune surveillance. PD-L1 expression in LUAD affected, among others, the metabolic trafficking between tumor and microenvironment (TME). Methods: Correlation iron content within TME was established on FFPE tissue samples. effects of an rich mRNA protein levels were assessed vitro H460 A549 using qPCR, western blot flow citometry. c-Myc knockdown performed validate role this transcription factor...
BACKGROUND Despite the abundance of studies on beneficial effects a fiber rich diet as well polyphenols deriving from Citrus fruits postprandial serum glucose and insulin, clinical evidence their synergic healthy subjects have never been published. We aimed to investigate feasibility new dietary approach in controlling insulin response at breakfast time by testing brioche enriched with wheat bran bergamot fiber. METHODS enrolled 11 volunteers cross-over study. Participants consumed classic...
Abstract Background Emerging evidence points toward the idea that oral squamous cell carcinoma (OSCC) invasiveness can be attributed to existence of a small subpopulation cancer stem cells (CSCs) in bulk tumor. However, knowledge about CSCs OSCC close margins and their possible role determining phenotypes is still far from being satisfactory. Methods We analyzed expression 13 marker genes 72 primary tissue specimens (24 core tumors, 24 pathologically negative margins, healthy distant...
Measuring 1,25-dihydroxyvitamin D (1,25(OH)2D), parathyroid hormone 1–84 (PTH 1–84) and intact FGF23 (iFGF23) is crucial for diagnosing a variety of diseases affecting bone mineral homeostasis. Biological variability (BV) data are important defining analytical quality specifications (APS), the usefulness reference intervals, significance variations in serial measurements same subject. The aim this study was to pioneer provision BV estimates 1,25(OH)2D improve existing iFGF23 PTH 1–84. Serum...
Abstract Aims Individuals with type 1 diabetes (T1D) do not appear to have an elevated risk of severe Coronavirus Disease 19 (COVID‐19). Pre‐existing immune reactivity Severe Acute Respiratory Syndrome 2 (SARS‐CoV‐2) in unexposed individuals may serve as a protective factor. Hence, our study was designed evaluate the existence T cells against SARS‐CoV‐2 antigens patients T1D. Materials and methods Peripheral blood mononuclear (PBMCs) were collected from T1D healthy control subjects. specific...