A5039304275- Lipid metabolism and biosynthesis
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- Extracellular vesicles in disease
- Endoplasmic Reticulum Stress and Disease
- RNA Research and Splicing
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- RNA Interference and Gene Delivery
- Hemoglobinopathies and Related Disorders
- Mesenchymal stem cell research
- Metal-Catalyzed Oxygenation Mechanisms
- Nanoplatforms for cancer theranostics
- Viral Infectious Diseases and Gene Expression in Insects
- Redox biology and oxidative stress
- Iron Metabolism and Disorders
- Metal complexes synthesis and properties
- Hemoglobin structure and function
- CRISPR and Genetic Engineering
- Hepatitis C virus research
- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- RNA modifications and cancer
German Cancer Research Center
2019-2024
Heidelberg University
2019-2024
Magna Graecia University
2014-2023
Heidelberg (Poland)
2020
Breast cancer is the most frequent in women worldwide and late diagnosis often adversely affects prognosis of disease. Radiotherapy commonly used to treat breast cancer, reducing risk recurrence after surgery. However, eradication radioresistant cells, including stem remains main challenge radiotherapy. Recently, lipid droplets (LDs) have been proposed as functional markers also being involved increased cell tumorigenicity. LD biogenesis a multistep process requiring various enzymes,...
Although much progress has been made in cancer treatment, the molecular mechanisms underlying radioresistance (RR) as well biological signatures of radioresistant cells still need to be clarified. In this regard, we discovered that breast, bladder, lung, neuroglioma, and prostate 6 Gy X-ray resistant were characterized by an increase lipid droplet (LD) number containing highest LDs showed clonogenic potential after irradiation. Moreover, observed LD content was tightly connected with iron...
Lipid Droplets (LDs) are emerging as crucial players in colon cancer development and maintenance. Their expression has been associated with high tumorigenicity Cancer Stem Cells (CSCs), so that they have proposed a new functional marker Colorectal (CR-CSCs). They also indirectly involved the modulation of tumor microenvironment through production pro-inflammatory molecules. There is growing evidence possible connection between metabolic alterations malignant transformation exists, although...
Gliomas are the most common type of primary brain tumors, presenting high mortality and recurrence rates that highlight need for development more efficient therapies. In context, we investigated iron(iii) (FeL) copper(ii) (CuL) complexes containing tetradentate ligand 2-{[(3-chloro-2-hydroxy-propyl)-pyridin-2-ylmethyl-amino]-methyl}-phenol (L) as potential antimetastatic compounds in glioma cells. These were designed to act mimetics antioxidant metalloenzymes (catalases superoxide dismutase)...
Solid tumors are often riddled with hypoxic areas, which develops as a result of high proliferation. Cancer cells willingly adapt and thrive in hypoxia by activating complex changes contributes to survival enhanced resistance treatments, such photon radiation. Photon radiation primarily relies on oxygen for the production reactive species induce DNA damage. The present in-vitro study aimed at investigating biochemical responses non-small cell lung cancer (NSCLC) cells, particularly effects...
Lentiviral vectors are widely used to investigate the biological properties of regulatory proteins and/or leukaemia-associated oncogenes by stably enforcing their expression in hematopoietic stem and progenitor cells. In these studies it is critical be able monitor sort infected cells, typically via fluorescent encoded modified viral genome. The most popular strategy ensure co-expression transgene reporter gene insert between cDNAs an IRES element, thus generating bi-cistronic mRNAs whose...
Transplantation of hematopoietic stem cells (HSCs) is a well-established therapeutic approach for numerous disorders. HSCs are typically derived from bone marrow or peripheral blood after cytokine-induced mobilization. Umbilical cord (CB) represents an appealing alternative HSC source, but the small amounts individual CB units have limited its applications. The availability strategies safe ex vivo expansion CB-derived (CB-HSCs) may allow to extend use these in adult patients and avoid risk...
Despite increasing knowledge about small extracellular vesicle (sEV) composition and functions in cell-cell communication, the mechanism behind their biogenesis remains unclear. Here, we reveal for first time that sEV release into microenvironment are tightly connected with another important organelle, Lipid Droplets (LDs). The correlation was observed several human cancer cell lines as well patient-derived colorectal stem cells (CR-CSCs). Our results demonstrated external stimuli such...
Whilst the impact of hypoxia and ionizing radiations on gene expression is well-understood, interplay these two effects not. To better investigate this aspect at level human bladder, brain, lung prostate cancer cell lines were irradiated with photons (6 Gy, 6 MV LINAC) in hypoxic normoxic conditions prepared for whole genome analysis 72 h post-irradiation. The was performed obtained 20,000 genes per line using PCA hierarchical cluster algorithms to extract most dominant altered by radiation...
We propose a novel mechanism by which cancer cells can modulate the oxygen concentration within nucleus, potentially creating low nuclear conditions without need of an hypoxic micro-environment and suited for allowing to resist chemo- radio-therapy. The ability alter intra-cellular depends on amount cholesterol present cellular membranes, where high levels yield rigid membranes that slow diffusion. proposed centers competition between (1) diffusion cell across replenishes any consumed (2)...
Abstract Despite an increasing gain of knowledge regarding small extracellular vesicle (sEV) composition and functions in cell-cell communication, the mechanism behind their biogenesis remains unclear. Here, we revealed for first time that sEV release into microenvironment are tightly connected with another important organelle: Lipid Droplets (LD). We have observed this correlation using different human cancer cell lines as well patient-derived colorectal stem cells (CR-CSCs). Our results...
Abstract Although much progress has been made in cancer treatment, the molecular mechanisms underlying radioresistance (RR) as well biological characteristic of radioresistant cells still need to be clarified. In this regard, we discovered that breast, bladder, lung, neuroglioma and prostate 6 Gy X-ray resistant were characterized by an increase Lipid Droplet (LD) number containing highest LDs showed clonogenic potential after irradiation. Moreover, observed LD content was tightly connected...