A5038221067- Lymphoma Diagnosis and Treatment
- CAR-T cell therapy research
- Extracellular vesicles in disease
- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Invertebrate Immune Response Mechanisms
- RNA Research and Splicing
- Protease and Inhibitor Mechanisms
- Ubiquitin and proteasome pathways
- Hematopoietic Stem Cell Transplantation
- Complement system in diseases
- Mesenchymal stem cell research
- HER2/EGFR in Cancer Research
- Cell death mechanisms and regulation
- Lipid metabolism and biosynthesis
- Glycosylation and Glycoproteins Research
- Sphingolipid Metabolism and Signaling
- Rheumatoid Arthritis Research and Therapies
- Platelet Disorders and Treatments
- Circular RNAs in diseases
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
German Cancer Research Center
2019-2024
Heidelberg University
2019-2024
La Ligue Contre le Cancer
2018
St. Jude Children's Research Hospital
2017-2018
Inserm
2018
Université Paris-Saclay
2018
Université Paris Cité
2016
Abstract Genome-wide association studies identified a single-nucleotide polymorphism (SNP) affecting the transcription factor Eomesodermin (EOMES) associated with significantly increased risk to develop chronic lymphocytic leukemia (CLL). Epigenetic analyses, RNA sequencing, and flow cytometry revealed that EOMES is not expressed in CLL cells, but CD8 + T cells for which known master regulator. We thus hypothesized SNP might be explained by its negative impact on T-cell-mediated immune...
The transcription factor eomesodermin (EOMES) promotes interleukin (IL)-10 expression in CD4+ T cells, which has been linked to immunosuppressive and cytotoxic activities. We detected cytotoxic, programmed cell death protein-1 (PD-1) EOMES co-expressing cells lymph nodes (LNs) of patients with chronic lymphocytic leukemia (CLL) or diffuse large B-cell lymphoma. Transcriptome flow cytometry analyses revealed that does not only drive IL-10 expression, but rather controls a unique...
Small extracellular vesicles (sEVs) are nanoparticles responsible for cell-to-cell communication released by healthy and cancer cells. Different roles have been described sEVs in physiological pathological contexts, including acceleration of tissue regeneration, modulation tumor microenvironment, or premetastatic niche formation, they discussed as promising biomarkers diagnosis prognosis body fluids. Although efforts made to standardize techniques isolation characterization sEVs, current...
Germline RUNX1 mutations lead to thrombocytopenia and platelet dysfunction in familial disorder with predisposition acute myelogenous leukemia (AML). Multiple aspects of function are impaired these patients, associated altered expression genes regulated by RUNX1. We aimed identify RUNX1-targets involved combining transcriptome analysis patient shRUNX1-transduced megakaryocytes (MK). Down-regulated included TREM-like transcript (TLT)-1 (TREML1) the integrin subunit alpha (α)-2 (ITGA2)...
Abstract Bispecific antibodies (BsAbs) can induce long-term responses in patients with refractory and relapsed B-cell lymphoma. Nevertheless, response rates across are heterogeneous, the factors determining quality duration of poorly understood. To identify key determinants to BsAbs, we established a primary, autologous culture model allowing us mimic treatment CD3xCD19 CD3xCD20 BsAbs within lymph node microenvironment ex vivo. T cell–mediated killing lymphoma cells proliferation varied...
Abstract Hematopoietic stem and progenitor cells (HSPCs) are necessary for life-long blood production replenishment of the hematopoietic system during stress. We recently reported that nuclear factor I/X (Nfix) promotes HSPC survival post-transplant. Here, we report ectopic expression Nfix in primary mouse HSPCs extends their ex vivo culture from about 20 to 40 days. overexpressing display hypersensitivity supportive cytokines reduced apoptosis when subjected cytokine deprivation relative...
Despite increasing knowledge about small extracellular vesicle (sEV) composition and functions in cell-cell communication, the mechanism behind their biogenesis remains unclear. Here, we reveal for first time that sEV release into microenvironment are tightly connected with another important organelle, Lipid Droplets (LDs). The correlation was observed several human cancer cell lines as well patient-derived colorectal stem cells (CR-CSCs). Our results demonstrated external stimuli such...
Abstract The transcription factor Eomesodermin (EOMES) promotes IL-10 production of CD4 + T-cells, which has been linked to immunosuppressive and cytotoxic activities. We detected EOMES-expressing T-cells in lymph node samples patients with chronic lymphocytic leukemia (CLL) or diffuse large B-cell lymphoma. This was line an observed expansion EOMES-positive leukemic Eµ-TCL1 mice, a well-established model CLL, upon adoptive transfer TCL1 mice. Transcriptome flow cytometry analyses revealed...
Introduction: Little is known about intra-tumor heterogeneity of lymphoma and their complex disease-specific lymph node (LN) microenvironment. Methods: To address this gap knowledge we characterized follicular lymphoma, diffuse large B cell (DLBCL) reactive LN biopsies by comprehensive immunophenotyping (29 markers, n=40) single RNA sequencing (scRNA-Seq, n=7). We used state-of-the-art clustering algorithms to identify malignant subclones separated them fluorescence-activated sorting (FACS)....
Abstract Despite an increasing gain of knowledge regarding small extracellular vesicle (sEV) composition and functions in cell-cell communication, the mechanism behind their biogenesis remains unclear. Here, we revealed for first time that sEV release into microenvironment are tightly connected with another important organelle: Lipid Droplets (LD). We have observed this correlation using different human cancer cell lines as well patient-derived colorectal stem cells (CR-CSCs). Our results...
Abstract Tumor heterogeneity encompasses both the malignant cells and their microenvironment. While between individual patients is well-known to affect efficacy of anti-cancer drugs, most personalized treatment approaches do not account for intratumor heterogeneity. We addressed this issue by studying lymph node-derived B cell non-Hodgkin lymphoma (B-NHL) single RNA-sequencing (scRNA-seq) transcriptome-informed flow cytometry. identified transcriptionally distinct subclones compared drug...