A5089520479- Oral microbiology and periodontitis research
- Oral Health Pathology and Treatment
- Periodontal Regeneration and Treatments
- Oral and gingival health research
- Education, Psychology, and Social Research
- Salivary Gland Disorders and Functions
- Diabetes and associated disorders
- Immune Response and Inflammation
- Dental Implant Techniques and Outcomes
- Autoimmune Bullous Skin Diseases
- Endodontics and Root Canal Treatments
- Oral and Maxillofacial Pathology
- Nail Diseases and Treatments
- Dental Radiography and Imaging
- Streptococcal Infections and Treatments
- Protease and Inhibitor Mechanisms
- Medical and Biological Sciences
- Dental Trauma and Treatments
- Skin and Cellular Biology Research
- Dental Health and Care Utilization
- Pediatric health and respiratory diseases
- Asthma and respiratory diseases
- Bone and Dental Protein Studies
- Tumors and Oncological Cases
- Laser Applications in Dentistry and Medicine
Masaryk University
2011-2024
St. Anne's University Hospital Brno
2008-2024
University Hospital Brno
2009-2015
Stomatology Hospital
2003
Background: Chronic periodontitis is an inflammatory disease, which a major cause of tooth loss. The proinflammatory cytokines interleukin‐1 (IL‐1) and interleukin‐6 (IL‐6) are key regulators the host response to microbial infection modulators extracellular matrix catabolism bone resorption. purpose this study was investigate associations chronic with IL‐6 gene polymorphisms (at positions –597 [G/A], –572 [G/C], –174 [G/C]). Methods: We analyzed allele, genotype, haplotype distributions...
Recurrent aphthous stomatitis (RAS) is the most common disease of oral mucosa, and it has been recently associated with bacterial fungal dysbiosis. To study this link further, we investigated microbial shifts during RAS manifestation at an ulcer site, in its surroundings, unaffected compared healed mucosa patients healthy controls. We sampled microbes from five distinct sites cavity. The one site pronounced differences alpha beta diversity between controls was lower labial mucosa. Detailed...
Abstract Background Glutathione S-transferases (GSTs) play important roles in protecting cells against oxidative stress and toxic chemicals. This study aimed to investigate the distribution of GSTM1 , GSTT1 GSTP1 variants their periodontitis susceptibility a Caucasian population. Methods We analyzed 406 participants, including 204 healthy controls 203 patients. A multiplex polymerase chain reaction (PCR) approach was used analyze loci. were detected by PCR-haplotyping method subgroup...
Interleukin-17 contributes to the pathogenesis of type 1 diabetes mellitus (T1DM) and chronic periodontitis (CP). We analyzed IL-17A −197A/G IL-17F +7488C/T polymorphisms in T1DM CP determined their associations with IL-17 production occurrence periopathogens. Totally 154 controls, 125 T1DM, 244 patients were genotyped using 5′ nuclease<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msup><mml:mrow><mml:mi mathvariant="normal">T</mml:mi><mml:mi...
Background: Periodontitis is an inflammatory disease that leads to irreversible attachment loss, bone destruction and eventually tooth loss. Tumor necrosis factor (TNF), a pluripotent proinflammatory cytokine able induce tissue resorption, has been implicated in the pathogenesis of periodontal disease. Methods: In this study, we investigated association between chronic periodontitis two previously described bi‐allelic polymorphisms TNF locus: G A transition at position −308 5′promoter region...
Abstract Objectives: Matrix metalloproteinase‐1 (MMP‐1) is a potent enzyme degrading extracellular matrix that was implicated in the pathogenesis of chronic periodontitis. Therefore, aim our study to examine association between three promoter polymorphisms MMP‐1 gene and periodontitis susceptibility and/or severity Czech population. Materials Methods: A total 329 Caucasian subjects were enrolled this study. They 133 patients with mild severe 196 unrelated control subjects. (−1607 1G/2G,...
Background: Cytokine gene polymorphisms are known to influence the susceptibility and disease course of many chronic disorders. Recently, interleukin (IL)‐4 were associated with aggressive periodontitis. The aim this study was test differences in distribution IL‐4 alleles, genotypes, haplotypes between patients periodontitis (CP) healthy controls a Czech population. Methods: association conducted using an age‐ smoking status–matched case‐control design CP (n = 194) 158) polymerase chain...
Abstract Objectives: Transforming growth factor–β (TGF‐beta) represents a family of polypeptide factors, involved in inflammation and regulation immune responses. The purpose this study was to determine whether polymorphisms the TGF‐beta 1 gene may confer susceptibility adult periodontitis. Material methods: We studied 90 patients with periodontitis together 108 unrelated subjects. 3 located 5′region at positions −988 (C/A), −800 (G/A) −509 (C/T) 2 codons 10 (L10P) 25 (R25P) exon were...
Members of the matrix metalloproteinase (MMP) family are implicated in chronic remodeling periodontal diseases. Therefore, we performed a case-control study to investigate plausible association between susceptibility periodontitis (CP) and polymorphisms MMP-9 (gelatinase B) gene.Using polymerase chain reaction with subsequent restriction analyses, -1562C/T R+279Q variants were determined 304 subjects (169 patients CP, 76 mild moderate form 93 severe generalized 135 age- gender-matched...
Toll-like receptors (TLRs) belong to the pattern recognition family of signal molecules that recognize conserved microbial structures. The aim this study was analyse polymorphisms in TLR genes and their association with chronic periodontitis (CP).Two (2408G/A, i.e. Arg753Gln -16934A/T) TLR-2 three variants (-1486C/T, -1237C/T and+2848A/G) TLR-9 were studied 222 patients CP 259 unrelated controls. All detected using polymerase chain reaction-restriction fragment length polymorphism methods....
Objectives . Periodontitis is an inflammatory disease characterized by connective tissue loss and alveolar bone destruction. Interleukin-8 ( IL8 ) important in the regulation of immune response. The aim this study was to analyze four polymorphisms gene relation chronic (CP) aggressive (AgP) periodontitis. Methods A total 492 unrelated subjects were included case-control association study. Genomic DNA 278 patients with CP, 58 AgP, 156 controls genotyped, using 5′ nuclease TaqMan assay, for...
Host genetic predispositions to dysregulated immune response can influence the development of aggressive form periodontitis (AgP) through susceptibility oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed perform a multilocus analysis functional variants in selected interleukin (IL) genes patients with generalized AgP homogenous population. Twelve polymorphisms IL-1 gene cluster, IL-6 its receptor, IL-10, IL-17A, IL-18 were determined 91 210 controls....
Background: Periodontal diseases are viewed today as multifactorial problems initiated and sustained by bacteria but significantly modified the body's response to bacterial plaque. A recent study suggested that receptor for advanced glycation end products (RAGE) could be involved in pathophysiology of periodontitis. The aim this was investigate a possible association 3 common polymorphisms RAGE gene with chronic Methods: We studied 101 Caucasian patients periodontitis together 162 orally...
Background: Gelatinase A (matrix metalloproteinase‐2 [MMP‐2]) has been shown to play an important role in the pathogenesis of several disorders, including periodontal diseases. In this study, we test hypothesis that variations gene influence development and severity chronic periodontitis. Methods: Four promoter polymorphisms (‐1575G/A, ‐1306C/T, ‐790T/G, ‐735C/T) were analyzed by polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) methods 149 patients with mild...
Chronic periodontitis (CP) is an inflammatory disease of the teeth-supporting tissues in which genetic predisposition, dental plaque bacteria, and immune mechanisms all play important roles. The aim this study was to evaluate occurrence IL-4 gene polymorphisms chronic investigate association between cytokines production after bacterial stimulation. Sixty-two subjects (47 CP patients 15 healthy controls) with detected two (-590C/T intron 3 VNTR) were examined. Production (IL-1 α , IL-1 β...
Background: Adult periodontitis is a complex multifactorial disease whose etiology not well defined. To investigate whether the genes encoded within HLA class III region may confer susceptibility to periodontitis, polymorphisms in ET‐1 and TNF‐β were analyzed together with I/D polymorphism of ACE gene. Methods: We determined allele genotype frequencies NcoI bi‐allelic gene, (insertion/deletion) TaqI gene 63 Caucasian patients adult 95 orally healthy controls. Results: found significant...
Background and Objective: Periodontitis is a bacterially induced chronic inflammatory disease major cause of tooth loss among adults. Toll‐like receptors are signal molecules essential for the cellular response to bacterial cell wall components. The aim this study was investigate relationships between periodontitis variations in TLR4 gene. Material Methods: A total 171 patients with 218 unrelated controls were genotyped Asp299Gly (896A>G) Thr399Ile (1196C>T) polymorphisms Results: Both...
Recent studies have suggested a bidirectional relationship between chronic periodontitis (CP) and diabetes mellitus (DM). Immunoregulatory factors such as cytokines play an important role in etiopathogenesis of both diseases. The aim this study was to analyze variability interleukin-1 ( IL-1 ) gene cluster β plasma levels patients with CP, DM, combination A total 1016 individuals participating case-control study—225 healthy controls, 264 132 type 1 (T1DM), 395 2 (T2DM)—were genotyped using...
Recurrent aphthous stomatitis (RAS) is a multifactorial disease with unclear etiopathogenesis in which disturbance of immunological processes may be involved. The aim our study was to investigate three single nucleotide polymorphisms (SNPs) rs3806265, rs4612666, rs10754558 NOD-like receptor 3 (NLRP3), the gene encoding component inflammasome, patients RAS and healthy controls Czech population.A total 207 subjects were included this case-control study. Sixty-four 143 genotyped by method based...
Specific variants in genes that encode adipokines and their mRNA protein expression were previously studied type 2 diabetes mellitus (T2DM) obesity, similar studies have been performed for chronic periodontitis (CP). The aim of this case-control study was to investigate the possible impacts adiponectin (ADIPOQ), leptin (LEP) its receptor (LEPR), resistin (RETN) on etiopathogenesis CP. Examinations 118 non-periodontitis healthy subjects (healthy controls, HC), 205 individuals with CP (H + CP)...
Genetic factors, especially those related to immune system functioning, have been intensively studied determine their role in the development of recurrent aphthous stomatitis (RAS). The aim present study was analyze gene variability interleukin ( IL ) 2 , IL4 (and its receptor α, IL4Rα IL10 and IL13 which were selected based on literature review and/or functional relevance, Czech patients with RAS healthy controls. In total, 252 subjects (178 controls 74 RAS) enrolled this case–control...